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p62/SQSTM1 Antibody - BSA Free

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Biological Strategies: Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - HeLa cells were treated overnight with 50uM CQ, then fixed for 10 ...read more
Simple Western: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Lane view shows a specific band for p62/SQSTM1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more
Biological Strategies: Western Blot: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated with or without 50 uM chloriquine for 24 hours as indicated. ...read more
Biological Strategies: Western Blot: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Immunoblotting analysis of LC3 and p62/SQSTM1 expression in thioglycollate-elicited peritoneal ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Untreated HeLa cells were fixed for 10 minutes using 10% formalin ...read more
Biological Strategies: Western Blot: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Vacuolin-1 analogues identified via virtual screening inhibited autophagic flux in HeLa cells. ...read more
Western Blot: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - p62/SQSTM1 Antibody [NBP1-48320] - Vacuolin-1 analogues identified via virtual screening induced the accumulation of both LC3B-II and SQSTM1 in HeLa cells in a ...read more
Simple Western: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - Drp1+/- mice is protective against autophagy impairment induced by Mn. (A) Representative images of the coronal mouse midbrain section (20um) ...read more
Simple Western: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - Drp1+/- mice is protective against autophagy impairment induced by Mn. (A) Representative images of the coronal mouse midbrain section (20um) ...read more
Autophagy associated protein immunoreactivity in HIV-infected brain tissue. (A) Representative images from five randomly selected fields of cells each examined in duplicate frontal lobe white matter sections for the ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody - BSA Free [NBP1-48320] - Effects on autophagic activity & dendritic length of neurons exposed to supernatant from HIV-1-infected microglia in combination ...read more
Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal p62/SQSTM1 Antibody [NBP1-48320] - Mice hepatocytes stained with p62/SQSTM1 Antibody. Image from a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Po, Bv, Ca, ChHa, In, ShSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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p62/SQSTM1 Antibody - BSA Free Summary

Immunogen
Partial recombinant protein made to the C terminal of the human p62/SQSTM1 protein (within residues 300-440). [Swiss-Prot Q13501]
Localization
Cytoplasm. Late endosome. Nucleus.
Predicted Species
Porcine (96%), Bovine (97%), Canine (91%), Invertebrate (97%), Sheep (98%), Chinese Hamster (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SQSTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:25-1:200
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 35389045)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Simple Western 1:25
  • Western Blot 1:4000
Control
Biliverdin Reductase A/BLVRA Antibody (4G4-2B6)
Reviewed Applications
Read 8 Reviews rated 4.8
using
NBP1-48320 in the following applications:

Publications
Read Publications using
NBP1-48320 in the following applications:

Reactivity Notes

Use in Chicken reported in scientific literature (PMID:33360697) Immunogen sequence is 100% identical to several non-human primates/monkey species. Immunogen displays the following percentage of sequence identity for non-tested species: Chinese hamster (98%), Sheep (98%), Bovine (97%), Porcine (96%), Canine (91%), Daphnia magna, a cladoceran/crustaceans invertebrate (97%) and Duck and several other Birds (84%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p62/SQSTM1 Antibody - BSA Free

  • A170
  • Autophagy Receptor P62
  • DMRV
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • FTDALS3
  • NADGP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PKC iota/lambda through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p62/SQSTM1 Antibody (NBP1-48320)(127)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Porcine.

We have publications tested in 12 applications: B/N, Block/Neutralize, FLOW, ICC, ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, Immunocytochemistry/ Immunofluorescence, WB, Western Blot.


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B/N
(1)
Block/Neutralize
(1)
FLOW
(1)
ICC
(1)
ICC/IF
(12)
IF/IHC
(2)
IHC-Fr
(1)
IHC-FrFl
(1)
IHC-P
(3)
Immunocytochemistry/ Immunofluorescence
(1)
WB
(73)
Western Blot
(3)
All Applications
Filter By Species
Human
(46)
Mouse
(38)
Rat
(1)
Chicken
(3)
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(1)
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Showing Publications 1 - 10 of 127. Show All 127 Publications.
Publications using NBP1-48320 Applications Species
Tedeschi V, Nele V, Valsecchi V et al. Nanoparticles Encapsulating Phosphatidylinositol Derivatives Promote Neuroprotection and Functional Improvement via a long-lasting activation of TRPML1 lysosomal channel in Preclinical Models of ALS. Pharmacological research 2024-11-02 [PMID: 39491634]
Xianzun Tao, Jiaqi Liu, Zoraida Diaz-Perez, Jackson R. Foley, Ashley Nwafor, Tracy Murray Stewart, Robert A. Casero, R. Grace Zhai Reduction of spermine synthase enhances autophagy to suppress Tau accumulation Cell Death & Disease 2024-05-13 [PMID: 38740758]
Wenting You, Kèvin Knoops, Tos T. J. M. Berendschot, Birke J. Benedikter, Carroll A. B. Webers, Chris P. M. Reutelingsperger, Theo G. M. F. Gorgels PGC-1a mediated mitochondrial biogenesis promotes recovery and survival of neuronal cells from cellular degeneration Cell Death Discovery 2024-04-17 [PMID: 38632223]
Amanda JM, Taylor ED, Artem NP et al. Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D Autophagy. 2024-04-09 [PMID: 38591519] (Western Blot, Mouse) Western Blot Mouse
Kyeong Eun Yang, Soo-Bin Nam, Ga-Eun Lee, Gabsik Yang, Mee-Hyun Lee, Geul Bang, Jung Hoon Choi, Yong-Yeon Cho, Cheol-Jung Lee, Jung-Rae Rho Induction of Autophagy by Extract from Corydalis heterocarpa for Skin Anti-Aging Marine Drugs 2024-03-08 [PMID: 38535468]
Rebecca Z. Fan, Carolina Sportelli, Yanhao Lai, Said S. Salehe, Jennifer R. Pinnell, Harry J. Brown, Jason R. Richardson, Shouqing Luo, Kim Tieu A partial Drp1 knockout improves autophagy flux independent of mitochondrial function Molecular Neurodegeneration 2024-03-19 [PMID: 38504290]
Clara Bayona, LĂ­a Alza, Teodora RanÄ‘elović, Marta C. SallĂ¡n, Anna Visa, Carles CantĂ­, Ignacio Ochoa, Sara OlivĂ¡n, Judit Herreros Tetralol derivative NNC-55-0396 targets hypoxic cells in the glioblastoma microenvironment: an organ-on-chip approach Cell Death & Disease 2024-02-10 [PMID: 38341408]
Fulong Yu, Liang Li, Yimei Gu, Song Wang, Lianbang Zhou, Xiaohu Cheng, Heng Jiang, Yang Huang, Yingfeng Zhang, Wenbao Qian, Xianghua Li, Zhining Liu Lysine demethylase 5C inhibits transcription of prefoldin subunit 5 to activate c-Myc signal transduction and colorectal cancer progression Molecular Medicine 2024-01-12 [PMID: 38216914]
Abdalla Elbialy, Mai Kitauchi, Dai Yamanouchi, Anne Vejux Antioxidants and azd0156 Rescue Inflammatory Response in Autophagy-Impaired Macrophages International Journal of Molecular Sciences 2023-12-21 [PMID: 38203340]
Yuan J, Zhao F, Liu Y et al. Effects of Lactiplantibacillus plantarum on oxidative stress, mitophagy, and NLRP3 inflammasome activation in broiler breast meat Poultry Science 2023-09-20 [PMID: 37832190] (WB, Chicken) WB Chicken
Show All 127 Publications.

Reviews for p62/SQSTM1 Antibody (NBP1-48320) (8) 4.88

Average Rating: 4.8
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-48320:
Filter by Applications
IF
(1)
WB
(5)
ICC
(1)
IHC-P
(1)
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Filter by Species
Human
(6)
Mouse
(2)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Armen Petrosyan
IF Mouse 11/19/2024
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotB-2
Western Blot p62/SQSTM1 NBP1-48320
Enlarge
5
reviewed by:
Verified Customer
WB Human 12/11/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain tissue
SpeciesHuman
LotB
Western Blot p62/SQSTM1 NBP1-48320
Enlarge
5
reviewed by:
Verified Customer
WB Human 10/30/2017
View

Summary

ApplicationWestern Blot
Sample Testedhead and neck squamous cell carcinoma,Human HNSCC cell lines CAL27
SpeciesHuman
LotNBP1-48320
Immunocytochemistry p62/SQSTM1 NBP1-48320
Enlarge
5
reviewed by:
Judit Herreros
ICC Human 09/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedU87MG cells treated with chloroquine
SpeciesHuman
LotNBP1-48320SS
Immunohistochemistry-Paraffin p62/SQSTM1 NBP1-48320
Enlarge
5
reviewed by:
Judit Herreros
IHC-P Human 09/01/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman U87MG glioblastoma cells xenograft following cloroquine treatment
SpeciesHuman
LotNBP1-48320SS
  4
reviewed by:
Verified Customer
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23931340
SpeciesHuman
Western Blot p62/SQSTM1 NBP1-48320
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 11/15/2013
View

Summary

ApplicationWestern Blot
SpeciesMouse
  5
reviewed by:
Verified Customer
WB Human 12/23/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (NBP1-48320). (Showing 1 - 1 of 1 FAQs).

  1. This technical query is related to Cat. No.: NBP1-48320. Technical Concern: As per the technical data sheet, p62/ SQSTM1 band is seen at ~47 kDa. We expect the band to be seen at 62 KDa for the domain p62/ SQSTM1, however only a ~47kDa band is visible in our blot, is there any explanation for this observation?
    • Regarding your concern with the performance of our p62 antibody (NBP1-48320), it is expected to see multiple bands depending on what form of p62 is present in the testing sample. According to uniprot, p62 has two different isoforms at 47kDa and 38kDa, however due to the interaction of p62 with other proteins and some possible modifications, a 62kDa band is usually observed on the blot. There is an abundance of supporting literature suggesting this antibody is performing well. p62 is considered to be the marker for Autophagy and the protein undergoes massive post translational modifications which can explain size increase. There are 13 phosphorylation sites on this protein. Here is the uniprot link for p62 (Q13501 reference 30); (PubMed 15592455).

Control Lysate(s)

Secondary Antibodies

 

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By Christina Towers, PhD. Parkinson's disease is a debilitating neurodegenerative condition defined by the accumulation of alpha-synuclein-containing (alpha-SYN) intra-cytoplasmic inclusions, called Lewy bodies. The ...  Read full blog post.

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Recent Reviews

4.8
5
6
4
2
3
0
2
0
1
0

Armen Petrosyan
11/19/2024
Application: IF
Species: Mouse

Verified Customer
12/11/2017
Application: WB
Species: Human

Verified Customer
10/30/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot