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Src [p Tyr419] Antibody [Unconjugated]

 (10 Publications)
 (1 Review)
 
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Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 10 minutes. PVDF membrane was ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver array using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at ...read more
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Yap heterogeneity is unrelated to markers examinedAll panels show examples of confocal stains of lung slices from KP mice, 14 weeks after infection. (A, B) Adjacent sections stained for E-cad, ZO-1, and either Yap (A), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF2685-SP
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Src [p Tyr419] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Src Y419 site
Modification
p Tyr419
Specificity
Detects human Src family members when phosphorylated at sites corresponding to Y419 of human Src in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRC
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF2685 in the following applications:
Publications
Read Publications using
AF2685 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Src [p Tyr419] Antibody [Unconjugated]

  • ASV
  • c-Src
  • EC 2.7.10
  • EC 2.7.10.2
  • pp60c-src
  • Rous sarcoma
  • RSVgp4
  • Src
  • tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src and other Src family protein tyrosine kinases are proto-oncogenes that play key roles in regulating cell growth and differentiation. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation at Y419 of human Src up-regulates kinase activity, while phosphorylation at Y530 inactivates Src.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Src [p Tyr419]
Antibody [Unconjugate
AF2685
Src [p Tyr419] Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC

Publications for Src Antibody (AF2685)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-Fr, IHC-P, Western Blot.


Filter By Application
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(1)
IHC-P
(1)
Western Blot
(4)
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(5)
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(2)
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Showing Publications 1 - 10 of 10.
Publications using AF2685 Applications Species
Ana Ruiz-Saenz, Chloe E. Atreya, Changjun Wang, Bo Pan, Courtney A. Dreyer, Diede Brunen et al. A reversible SRC-relayed COX2 inflammatory program drives resistance to BRAF and EGFR inhibition in BRAFV600E colorectal tumors Nature Cancer 2/9/2023 [PMID: 36759733]
Lisa Rösch, Sonja Herter, Sara Najafi, Johannes Ridinger, Heike Peterziel, Jindrich Cinatl et al. ERBB and P‐glycoprotein inhibitors break resistance in relapsed neuroblastoma models through P‐glycoprotein Molecular Oncology 1/1/2023 [PMID: 36181342]
Yen-Lin Huang, Ching-Yeu Liang, Danilo Ritz, Ricardo Coelho, Dedy Septiadi, Manuela Estermann et al. Collagen-rich omentum is a premetastatic niche for integrin alpha 2-mediated peritoneal metastasis eLife 10/7/2020 [PMID: 33026975]
J Ramírez-Ri, E Leal-Orta, E Martínez-B, C Ortiz-Mend, F Breton-Mor, A Herrera-To, I Elizalde-A, P Cortes-Rey, R Thompson-B, E Perez Sala Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src?dependent pathway in MDA?MB?231 breast cancer cells Mol Med Rep, 2020-06-18;0(0):. 2020-06-18 [PMID: 32582965] (Western Blot, Human) Western Blot Human
Y Mao, S Sun, KD Irvine Role and regulation of Yap in KrasG12D-induced lung cancer Oncotarget, 2017-12-02;8(67):110877-110889. 2017-12-02 [PMID: 29340023] (IHC-Fr, Mouse) IHC-Fr Mouse
Eri Takiguchi, Masato Nishimura, Ayuka Mineda, Takako Kawakita, Akiko Abe, Minoru Irahara Growth inhibitory effect of the Src inhibitor dasatinib in combination with anticancer agents on uterine cervical adenocarcinoma cells Experimental and Therapeutic Medicine 8/28/2017 [PMID: 29067110]
JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-03-08;0(0):. 2017-03-08 [PMID: 28286026] (Western Blot, Human) Western Blot Human
Cahoon J, Rai R, Carroll L, Uehara H, Zhang X, O'Neil C, Medina R, Das S, Muddana S, Olson P, Nielson S, Walker K, Flood M, Messenger W, Archer B, Barabas P, Krizaj D, Gibson C, Li D, Koh G, Gao G, Stitt A, Ambati B Intravitreal AAV2.COMP-Ang1 Prevents Neurovascular Degeneration in a Murine Model of Diabetic Retinopathy. Diabetes, 2015-09-04;64(12):4247-59. 2015-09-04 [PMID: 26340930] (Western Blot, Human) Western Blot Human
Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. J Virol, 2013-03-27;87(11):6326-35. 2013-03-27 [PMID: 23536671] (Western Blot, Human) Western Blot Human
Matsuo K, Nishimura M, Bottsford-Miller JN, Huang J, Komurov K, Armaiz-Pena GN, Shahzad MM, Stone RL, Roh JW, Sanguino AM, Lu C, Im DD, Rosenshien NB, Sakakibara A, Nagano T, Yamasaki M, Enomoto T, Kimura T, Ram PT, Schmeler KM, Gallick GE, Wong KK, Frumovitz M, Sood AK Targeting Src in Mucinous Ovarian Carcinoma. Clin. Cancer Res., 2011-07-07;17(16):5367-5378. 2011-07-07 [PMID: 21737505] (IHC-P, Human, Mouse) IHC-P Human, Mouse

Review for Src Antibody (AF2685) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2685:
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Images Ratings Applications Species Date Details
Western Blot Src AF2685
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 10/11/2018
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Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotVEF0615091

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Western Blot Src AF2685
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Application: WB
Species: Human
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Bioinformatics

Gene Symbol SRC