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PIK3CA Antibody

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Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody ...read more
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.
Western Blot: PIK3CA Antibody [NBP2-19804] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PIK3CA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p110 alpha. The exact sequence is proprietary.
Predicted Species
Rat (99%), Chicken (95%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3CA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-19804 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for PIK3CA Antibody

  • CLAPO
  • CLOVE
  • CWS5
  • EC 2.7.1
  • EC 2.7.1.153
  • MCMTC
  • MGC142161
  • MGC142163
  • P110alpha
  • p110-alpha
  • Phosphatidylinositol 3-Kinase catalytic subunit alpha
  • phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • PI3K
  • PI3Kalpha
  • PI3K-alpha
  • PI3-kinase p110 subunit alpha
  • PI3-kinase subunit alpha
  • PIK3CA
  • PtdIns-3-kinase p110
  • PtdIns-3-kinase subunit alpha
  • PtdIns-3-kinase subunit p110-alpha

Background

PIK3CA Antibody is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PIK3CA Antibody
NBP2-19804
PIK3CA Antibody
Species: Hu, Mu, Rt, Bv, Ch, RM
Applications: WB, ICC/IF, IHC

Publications for PIK3CA Antibody (NBP2-19804)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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(2)
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Product General Protocols

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Bioinformatics

Gene Symbol PIK3CA
Uniprot