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![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19804-img0009.jpg "Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NBP2-19804-img0004.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0006.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-19804-img0007.jpg)
![Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PIK3CA antibody (NBP2-19804) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19804_rabbit-polyclonal-pik3ca-antibody-910202420134031.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0011.jpg "Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0001.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0002.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0003.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0004.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0005.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0006.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0007.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0008.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0009.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-6D9-Immunohistochemistry-NBP2-46403-img0010.jpg)
![Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Western-Blot-NBP2-37511-img0003.jpg "Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (4F3) [NBP2-37511] - Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Immunocytochemistry-Immunofluorescence-NBP2-37511-img0004.jpg)
![Flow Cytometry: PIK3CA Antibody (4F3) [NBP2-37511] - Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Flow-Cytometry-NBP2-37511-img0005.jpg)
![Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-Western-Blot-NBP2-37511-img0002.jpg)
![ELISA: PIK3CA Antibody (4F3) [NBP2-37511] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PIK3CA-Antibody-4F3-ELISA-NBP2-37511-img0001.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0005.jpg "Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0001.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0002.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0003.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PIK3CA Antibody (CL8347) [NBP2-88915] - Analysis in human placenta and skeletal muscle tissues using NBP2-88915 antibody. Corresponding PIK3CA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8347-Immunohistochemistry-Paraffin-NBP2-88915-img0006.jpg)
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NB100-55408-img0002.jpg "Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human brain (cerebellar cortex) with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunohistochemistry-Paraffin-NB100-55408-img0001.jpg)
![Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate. All lanes: PIK3CA Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 125 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202411442616.jpg "Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate. All lanes: PIK3CA Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 125 kDa Observed band size: 110 kDa")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] - Staining of Hela Cells with PIK3CA Antibody (10E5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26384_rabbit-pik3ca-mab-10e5-282202412493610.jpg)
![Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-98814-img0002.jpg "Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-98814] - Immunofluorescence staining of PIK3CA in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PIK3CA polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98814-img0001.jpg)
![Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PIK3CA-Antibody-3G3-Proximity-Ligation-Assay-H00005290-M01-img0002.jpg "Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: PIK3CA Antibody (3G3) [H00005290-M01] - Detection limit for recombinant GST tagged PIK3CA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-3G3-Sandwich-ELISA-H00005290-M01-img0003.jpg)
![Western Blot: PIK3CA Antibody (CL8345) [NBP3-05502] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/PIK3CA-Antibody-CL8345-Western-Blot-NBP3-05502-img0001.jpg "Western Blot: PIK3CA Antibody (CL8345) [NBP3-05502] - Analysis in human cell line HDLM-2.")