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![Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59177-img0001.jpg "Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.")
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.](http://images.novusbio.com/fullsize/HeLa-Hypoxic---Normoxic-Tissue-Lysate-Western-Blot-NBP2-36452-img0002.jpg "Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.")
![Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-Western-Blot-NBP1-42569-img0004.jpg "Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].")
![SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-SDS-Page-NBP1-42569-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).")
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Western Blot: Human Testis Whole Tissue Lysate (Adult Normal) [NB820-59266] - Detection of NUT and GAPDH in Human Testis Whole Tissue Lysate (cat# NB820-59266) and rat testis lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Human-Testis-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59266-img0001.jpg "Western Blot: Human Testis Whole Tissue Lysate (Adult Normal) [NB820-59266] - Detection of NUT and GAPDH in Human Testis Whole Tissue Lysate (cat# NB820-59266) and rat testis lysate. Image provided via product review by verified customer.")
![Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Colon-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59205-img0001.jpg "Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.")
![SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]](http://images.novusbio.com/fullsize/Human-Colon-Whole-Tissue-Lysate-Whole-Normal-SDS-Page-NB820-59205-img0002.jpg)
![Western Blot: Human Brain Cerebral Meninges Whole Tissue Lysate (Adult Whole Normal) [NB820-59183] - Western blots of GAPDH using NB820-59183 lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Brain-Cerebral-Meninges-Whole-Tissue-Lysate-Adult-Whole-Normal-Western-Blot-NB820-59183-img0001.jpg "Western Blot: Human Brain Cerebral Meninges Whole Tissue Lysate (Adult Whole Normal) [NB820-59183] - Western blots of GAPDH using NB820-59183 lysates. Image from verified customer review.")
![Western Blot: Human Lung Whole Tissue Lysate (Adult Normal) [NB820-59239] - analysis of Beta Actin in human lung tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Lung-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59239-img0001.jpg "Western Blot: Human Lung Whole Tissue Lysate (Adult Normal) [NB820-59239] - analysis of Beta Actin in human lung tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.")
![Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Native-Western-Blot-NBL1-12174-img0003.jpg "Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.")
![Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12174-img0002.jpg)