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![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).")
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg)
![Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl Hydroxylase 3.](http://images.novusbio.com/fullsize/PHD3-HIF-Prolyl-Hydroxylase-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10156-img0003.jpg "Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl Hydroxylase 3.")
![Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.](http://images.novusbio.com/fullsize/HIF-2-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10286-img0002.jpg "Western Blot: HIF-2 alpha Overexpression Lysate (Adult Normal) [NBL1-10286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIF-2 alpha.")
![Western Blot: eNOS Overexpression Lysate (Adult Normal) [NBP2-10685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eNOS.](http://images.novusbio.com/fullsize/eNOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10685-img0002.jpg "Western Blot: eNOS Overexpression Lysate (Adult Normal) [NBP2-10685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eNOS.")
![Western Blot: iNOS Overexpression Lysate (Adult Normal) [NBL1-13721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for iNOS.](http://images.novusbio.com/fullsize/iNOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13721-img0002.jpg "Western Blot: iNOS Overexpression Lysate (Adult Normal) [NBL1-13721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for iNOS.")
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOX4.](http://images.novusbio.com/fullsize/NOX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04362-img0002.jpg "Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOX4.")
![Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] - Left - Empty vector transfection control lysate (HEKP293 cell lysate); Right - NOX4 Over-expression Lysate,](http://images.novusbio.com/fullsize/NOX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04362-img0001.jpg)
![Western Blot: TIMP4 Overexpression Lysate (Adult Normal) [NBL1-16927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIMP4.](http://images.novusbio.com/fullsize/TIMP4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16927-img0002.jpg "Western Blot: TIMP4 Overexpression Lysate (Adult Normal) [NBL1-16927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIMP4.")
![Western Blot: TIMP4 Overexpression Lysate (Adult Normal) [NBL1-16927] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TIMP4](http://images.novusbio.com/fullsize/TIMP4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16927-img0001.jpg)
![Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DEC1.](http://images.novusbio.com/fullsize/DEC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07974-img0002.jpg "Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DEC1.")
![Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BHLHE40](http://images.novusbio.com/fullsize/DEC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07974-img0001.jpg)