![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Concentration | 2.5 mg/ml |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. Denaturing agents have not been added. |
| Preservative | No Preservative |
| Concentration | 2.5 mg/ml |
Research Areas for HeLa Lysate (NB800-PC1)Find related products by research area.
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