![Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-Western-Blot-NBP1-42569-img0004.jpg "Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP, PAGE |
| Concentration | 2.5 mg/ml |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted. BME has not been added. |
| Concentration | 2.5 mg/ml |
| Publication using NBP1-42569 | Applications | Species |
|---|---|---|
| Agostini-Dreyer A, Jetzt AE, Stires H, Cohick WS. Endogenous IGFBP-3 mediates intrinsic apoptosis through modulation of Nur77 phosphorylation and nuclear export. Endocrinology 2015-09-04 [PMID: 26340041] (WB) | WB |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 03/13/2014 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-SDS-Page-NBP1-42569-img0001.jpg "SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.")