![Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Colon-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59205-img0001.jpg "Western Blot: Human Colon Whole Tissue Lysate (Adult Normal) [NB820-59205] - analysis of Beta Actin in human colon tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light chain HRP conjugated secondary antibody (Cat. #NB7549). Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Concentration | 5.0 mg/ml |
| Description | We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity | Total Protein - Human Adult Normal Tissue: Colon |
| Dilutions |
|
|
| Application Notes | Western Blot Electrophoresis |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration | 5.0 mg/ml |
| Type | Tissue |
| Life Stage | Adult |
| Tissue | Digestive/Waste |
| Protein State | Native |
| Tissue Condition | Normal |
| Subcellular Fraction | Whole |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
-(1-mg)_NB820-59205_11426.jpg)
Enlarge |
reviewed by:
Ming Lu |
WB | Human | 10/23/2014 |
Summary
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]](http://images.novusbio.com/fullsize/Human-Colon-Whole-Tissue-Lysate-Whole-Normal-SDS-Page-NB820-59205-img0002.jpg "SDS-Page: Human Colon Whole Tissue Lysate (Whole Normal) [NB820-59205]")
-(1-mg)_NB820-59205_11426.jpg)