I have a question regarding your product no. NB820-59177. I would like to use this lysate in an ELISPOT assay and I am not sure if this is possible. My particular question is if this product is sterile, i.e. can it be used for cell culture?

Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

May we ask how much we shall load by using NB820-59177 as positive control in WB?

We recommend loading 10-20ug of lysate per well for Western blot.

Is there a recommended expiration date for the Human Brain Whole Tissue Lysate, cat:NB820-59177, Lot: B703069?

You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

May we ask if the Human Brain Whole Tissue Lysate (cat. no. NB820-59177) is suitable for RNA extraction?

Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.

Please see this link to all of our normal human brain tissue lysates.

Human Brain Whole Tissue Lysate (Adult Whole Normal) (NB820-59177): Novus Biologicals

Name: Human Brain Whole Tissue Lysate (Adult Whole Normal)
Brand: Novus Biologicals
SKU: NB820-59177
Rating: 5 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ELISA
Concentration	5.0 mg/ml

Description	We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity	Total Protein - Human Adult Normal Tissue: Brain

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration	5.0 mg/ml

Type	Tissue
Life Stage	Adult
Tissue	Brain
Protein State	Native
Tissue Condition	Normal
Subcellular Fraction	Whole

Human Brain Whole Tissue Lysate (Adult Whole Normal)

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Human Brain Whole Tissue Lysate (Adult Whole Normal) Summary

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Publications for Brain Lysate (NB820-59177)(29)

Review for Brain Lysate (NB820-59177) (1) 51

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FAQs for Brain Lysate (NB820-59177). (Showing 1 - 5 of 5 FAQs).

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Recent Reviews

Publications using NB820-59177	Applications	Species
David A. Lawrence, Aishwarya Jadhav, Tapan K. Mondal, Kyle Carson, William T. Lee, Alexander H. Hogan, Katherine W. Herbst, Ian C. Michelow, Michael Brimacombe, Juan C. Salazar, Eleanor N. Fish Inflammatory and Autoimmune Aspects of Multisystem Inflammatory Syndrome in Children (MIS-C): A Prospective Cohort Study Viruses 2024-06-12 [PMID: 38932242]
Claudio Peter D’Incal, Elisa Cappuyns, Kaoutar Choukri, Kevin De Man, Kristy Szrama, Anthony Konings, Lina Bastini, Kim Van Meel, Amber Buys, Michele Gabriele, Ludovico Rizzuti, Alessandro Vitriolo, Giuseppe Testa, Fabio Mohn, Marc Bühler, Nathalie Van der Aa, Anke Van Dijck, R. Frank Kooy, Wim Vanden Berghe Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients Scientific Reports 2024-06-26 [PMID: 38926592]
Han MH, Baek JM, Min KW et al. DKK3 expression is associated with immunosuppression and poor prognosis in glioblastoma, in contrast to lower-grade gliomas BMC neurology 2023-05-06 [PMID: 37149563] (Western Blot)	Western Blot

Waters CS, Angenent SB, Altschuler SJ, Wu LF A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit Cell reports 2023-10-31 [PMID: 37851575] (WB)	WB
Chunder R, Heider T, Kuerten S The prevalence of IgG antibodies against milk and milk antigens in patients with multiple sclerosis Frontiers in immunology 2023-07-10 [PMID: 37492579]
Rassek K, I?ykowska K, ?urawek M et al. TMEM244 Is a Long Non-Coding RNA Necessary for CTCL Cell Growth International journal of molecular sciences 2023-02-09 [PMID: 36834942] (Western Blot)	Western Blot
Capano L Dissecting Tau Isoform Control and Modeling Tauopathies in Directly-Reprogrammed Neurons Thesis 2023-01-01
Capano L, Sato C, Ficulle E et al. Recapitulation of endogenous 4R tau expression and formation of insoluble tau in directly reprogrammed human neurons Cell Stem Cell 2022-06-01 [PMID: 35659876] (NAE, Human)	NAE	Human
Tapanes SA, Arizanovska D, Diaz MM et al. Inhibition of glial D-serine release rescues synaptic damage after brain injury Glia 2022-02-23 [PMID: 35195906]
Tang CT, Li YF, Chou CH et al. GRPEL2 Knockdown Exerts Redox Regulation in Glioblastoma International Journal of Molecular Sciences 2021-11-24 [PMID: 34884508] (Western Blot)	Western Blot
Puvvula PK, Yu Y, Sullivan KR et al. Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation Cell reports 2021-06-01 [PMID: 34077726]
Tacke S, Braune S, Rovituso DM et al. B-Cell Activity Predicts Response to Glatiramer Acetate and Interferon in Relapsing-Remitting Multiple Sclerosis Neurology(R) neuroimmunology & neuroinflammation 2021-05-01 [PMID: 33707177] (ESPOT)	ESPOT
Guihurt Santiago J, Burgos-Tirado N, Lafontaine DD, et al. Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion BMC cancer 2021-01-11 [PMID: 33430814] (WB)	WB
Sahai A, Bhandari R, Koupenova M et al. SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients ResearchSquare 2023-08-22 [PMID: 33398263] (Western Blot, SDS-Page)	Western Blot, SDS-Page
Chen HY, Lin CH, Teng SC Stress-induced p53 drives BAG5 cochaperone expression to control alpha-synuclein aggregation in Parkinson's disease Aging (Albany NY) 2020-10-21 [PMID: 33085644] (IP)	IP
Kuerten S, Lanz TV, Lingampalli N et al. Autoantibodies against central nervous system antigens in a subset of B cell-dominant multiple sclerosis patients Proc. Natl. Acad. Sci. U.S.A. 2020-09-01 [PMID: 32817492]
Parkhouse RME, Carpio A, Cortez MM et al. Anti-brain protein autoantibodies are detectable in extraparenchymal but not parenchymal neurocysticercosis J. Neuroimmunol. 2020-04-06 [PMID: 32305783] (MS, Human)	MS	Human
Liao WC, Liao CK, Tseng TJ et al. Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability Oncogenesis 2020-02-04 [PMID: 32019907] (WB, Human)	WB	Human
Hwang M, Han MH, Park HH et al. LGR5 and Downstream Intracellular Signaling Proteins Play Critical Roles in the Cell Proliferation of Neuroblastoma, Meningioma and Pituitary Adenoma Exp Neurobiol 2019-10-31 [PMID: 31698554]
Ventero MP, Fuentes-Baile M, Quereda C et al. Radiotherapy resistance acquisition in Glioblastoma. Role of SOCS1 and SOCS3 PLoS ONE 2019-02-27 [PMID: 30811476] (WB, Human)	WB	Human
Majumder P, Roy K, Singh BK et al. Cellular levels of growth factor receptor bound protein 2 (Grb2) and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech. 2017-03-30 [PMID: 28360125] (WB)	WB
Saito K, Ohta S, Kawakami Y et al. Functional analysis of KIF20A, a potential immunotherapeutic target for glioma. J Neurooncol 2017-03-01 [PMID: 28070829]
Wunsch M, Hohmann C, Milles B et al. The Correlation between the Virus- and Brain Antigen-Specific B Cell Response in the Blood of Patients with Multiple Sclerosis. Viruses. 2016-04-28 [PMID: 27120609] (ELISA)	ELISA
Rasmussen RD, Gajjar MK, Jensen KE et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol. 2016-01-08 [PMID: 26794465] (WB)	WB
Choi I, Kim B, Byun JW et al. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase. Nat Commun 2015-09-14 [PMID: 26365310] (IP)	IP
Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood. Hohmann C, Milles B, Schinke M Acta Neuropathol Commun [PMID: 25597707] (ELISA, Human)	ELISA	Human
Molina J, Rodriguez-Diaz R, Fachado A et al. Control of Insulin Secretion by Cholinergic Signaling in the Human Pancreatic Islet. Diabetes 2014-08-01 [PMID: 24658304] (WB, Human)	WB	Human
Kuerten S, Pommerschein G, Barth SK et al. Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood. Clin. Immunol. 2014-03-05 [PMID: 24607792] (ELISPOT, Human)	ELISPOT	Human
Show All 29 Publications. Collapse Publications.

	Ming Lu 10/03/2014
Application:	WB
Species:	Human