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PIK3CA Antibody (4F3) - BSA Free

Name: PIK3CA Antibody (4F3) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37511
Price: 169 USD

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Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody (4F3) [NBP2-37511] - Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: PIK3CA Antibody (4F3) [NBP2-37511] - Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).

Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

ELISA: PIK3CA Antibody (4F3) [NBP2-37511] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, CyTOF-ready
Clone	4F3
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

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PIK3CA Antibody (4F3) - BSA Free Summary

Immunogen	Purified recombinant fragment of human PIK3CA expressed in E. Coli.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	PIK3CA
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	CyTOF-ready ELISA 1:10000 Flow Cytometry 1:200 - 1:400 Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000 Western Blot 1:500 - 1:2000
Application Notes	This antibody is Cytof ready.
Theoretical MW	124 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Alternate Names for PIK3CA Antibody (4F3) - BSA Free

CLAPO
CLOVE
CWS5
EC 2.7.1
EC 2.7.1.153
MCMTC
MGC142161
MGC142163
P110alpha
p110-alpha
Phosphatidylinositol 3-Kinase catalytic subunit alpha
phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
phosphoinositide-3-kinase, catalytic, alpha polypeptide
PI3K
PI3Kalpha
PI3K-alpha
PI3-kinase p110 subunit alpha
PI3-kinase subunit alpha
PIK3CA
PtdIns-3-kinase p110
PtdIns-3-kinase subunit alpha
PtdIns-3-kinase subunit p110-alpha

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PIK3CA Antibody
(4F3) - BSA Free

NBP2-37511

Species: Hu

Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready

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Product General Protocols

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Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for PIK3CA Antibody (NBP2-37511) (0)

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Secondary Antibodies

Isotype Controls

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Bioinformatics

Gene Symbol

PIK3CA

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

PIK3CA Antibody (4F3) - BSA Free

Species Glossary

Images

Product Details

Order Details

PIK3CA Antibody (4F3) - BSA Free Summary

Applications/Dilutions

Packaging, Storage & Formulations

Alternate Names for PIK3CA Antibody (4F3) - BSA Free

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for PIK3CA Antibody (NBP2-37511) (0)

Reviews for PIK3CA Antibody (NBP2-37511) (0)

Product General Protocols

Video Protocols

FAQs for PIK3CA Antibody (NBP2-37511) (0)

Secondary Antibodies

Isotype Controls

Additional PIK3CA Products

Research Areas for PIK3CA Antibody (NBP2-37511)

Blogs on PIK3CA

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics