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AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

 (49 Publications)
 
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Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with ...read more
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody ...read more
Serum-starved MCF-7 human breast cancer cells were unstimulated (blue open histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained ...read more
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (291-G1) for 15 minutes, loaded ...read more
Phospho-proteome profiling results of LX-2 cells co-cultured with C3A–CYP2E1 cells with or without DDC treatment and detection of the effect of DDC on intracellular kinases in LX-2 cells of co-cultures200 μg of ...read more
Phospho-proteome profiling results of LX-2 cells co-cultured with C3A–CYP2E1 cells with or without DDC treatment and detection of the effect of DDC on intracellular kinases in LX-2 cells of co-cultures200 μg of ...read more
DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The ...read more
Enhancement of autophagy by SLAMF5 is not mediated by inhibition of the mTORC1 complex. (A) The autophagy-inducer rapamycin was applied to control or SLAMF5-silenced cells for 4 h. Autophagy was monitored by western ...read more
p38 inhibitor SB203580 improves the up-regulation of MMP-1 by DDC through stimulating ERK1/2Co-cultures were treated with or without 100 μM DDC for 1 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF887-SP
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AKT [p Ser473] Antibody [Unconjugated] - Pan Specific Summary

Immunogen
Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Modification
p Ser473
Specificity
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKT1
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF887 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

  • RAC-ALPHA
  • AKT serine/threonine kinase 1
  • Akt
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC

Background

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKT Antibody (AF887)(49)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate,  Human.

We have publications tested in 6 applications: Bioassay, ELISA Development, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(27)
All Applications
Filter By Species
Human
(18)
Mouse
(7)
Rat
(4)
N/A
(1)
Primate
(1)
 Human
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using AF887 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Zhang, FW;Xie, XW;Chen, MH;Tong, J;Chen, QQ;Feng, J;Chen, FT;Liu, WQ; Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells World journal of gastroenterology 2023-08-21 [PMID: 37664151] (Western Blot, Human) Western Blot  Human
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 2023-07-01 [PMID: 37457144]
Noor Tarawneh, Lama Hamadneh, Bashaer Abu-Irmaileh, Ziad Shraideh, Yasser Bustanji, Shtaywy Abdalla Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 Expressions Molecules 2023-04-29 [PMID: 37175233]
Sharanya Nag, Sayanur Rahaman, Abhishek Guha, Partho Sarothi Ray An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock iScience 2023-04-21 [PMID: 36968077]
OE Mohamed, DM Abdallah, AM Fayez, RA Mohamed, HS El-Abhar Morin post-treatment surpassed calpeptin in ameliorating 3-NP-induced cortical neurotoxicity via modulation of glutamate/calpain axis, Kidins220, and BDNF/TrkB/AKT/CREB trajectory International immunopharmacology, 2023-02-01;116(0):109771. 2023-02-01 [PMID: 36736222] (Western Blot, Rat) Western Blot Rat
OY Teran, MR Zanotelli, MJ Lin, RA Cerione, KF Wilson Dock7 regulates AKT and mTOR/S6K activity required for the transformed phenotypes and survival of cancer cells bioRxiv : the preprint server for biology, 2023-01-03;0(0):. 2023-01-03 [PMID: 36711811] (Bioassay, N/A) Bioassay N/A
Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 2022-09-08 [PMID: 36145344]
Zhou M, Zhou S, Han K et al. Th1 immune maturation effects of Nocardia rubra cell-wall skeleton via PI3K/Akt/PAX8 regulatory axis Science progress 4/27/2022 [PMID: 35473474]
JH Hwang, SY Kang, HW Jung Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway Molecular Medicine Reports, 2022-04-08;25(6):. 2022-04-08 [PMID: 35419614] (Western Blot, Mouse) Western Blot Mouse
Show All 49 Publications.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKT1