AKT Antibodies
We offer AKT Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot. Each AKT Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AKT Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the AKT Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AKT polyclonal antibodies and browse our AKT monoclonal antibody catalog.
Alternate Names for AKT Antibodies
anti-AKT antibody, anti-AKT1 antibody
12 Results for "akt" in Primary Antibodies
![Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-57202315545125.jpg "Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.")
![Western Blot: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720239533735.jpg)
![Immunocytochemistry/Immunofluorescence: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720231051266.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0004.jpg "Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Untreated (-) and treated (+) MCF-7 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Thr450) antibody (NBP3-13168) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0001.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0002.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0003.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0001.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0002.jpg)
![Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32747_rabbit-akt-p-ser473-mab-psh04-44-278202415242614.jpg "Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/AKT-p-Thr308-Antibody-2O2W6-Western-Blot-NBP3-16956-img0001.jpg "Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).](http://images.novusbio.com/fullsize/AKT-[p-Thr342]-Antibody-Western-Blot-NBP2-60772-img0001.jpg "Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).")