Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 7 applications: B/N, ELISA, Func, IF/IHC, IHC-P, In Vivo, WB.

Showing Publications 1 - 10 of 62. Show All 62 Publications. Collapse Publications.

Publications using NB7160	Applications	Species
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Ji Y, Liang Y, Chu PH Et al. The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice Biomed J 2022-10-16 [PMID: 36244649] (WB) Details: Citation using the HRP version of this antibody.	WB
Swamy G, Salo P, Duncan N Et al. IL-1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice JOR Spine 2022-07-05 [PMID: 35783913] (IHC-P) Details: Citation using the HRP version of this antibody.	IHC-P
Hendawy AS, El-Lakkany NM, Mantawy EM Et al. Vildagliptin alleviates liver fibrosis in NASH diabetic rats via modulation of insulin resistance, oxidative stress, and inflammatory cascades Life Sci 2022-06-07 [PMID: 35671811] (WB) Details: Citation using the HRP version of this antibody.	WB
Salem MA, Manaa EG, Osama N Et al. Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFb/SMAD pathway Sci Rep 2022-04-22 [PMID: 35449437] (WB) Details: Citation using the HRP version of this antibody.	WB
Kortam MA, Alawady AS, Hamid Sadik NA Et al. Fenofibrate mitigates testosterone induced benign prostatic hyperplasia via regulation of Akt/FOXO3a pathway and modulation of apoptosis and proliferation in rats Arch Biochem Biophys 2022-04-17 [PMID: 35430215] (WB) Details: Citation using the HRP version of this antibody.	WB
Wu X, Pang Q, Zhi F Et al. Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells Tissue Cell 2022-03-24 [PMID: 35325674] (WB) Details: Citation using the HRP version of this antibody.	WB
Karim S, Burzangi AS, Ahmad A Et al. PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer Int J Mol Sci 2022-02-26 [PMID: 35216429] Details: Citation using the HRP version of this antibody.
Abdel-Latif RT, Wadie W, Abdel-Mottaleb Y Et al. Reposition of the anti-inflammatory drug diacerein in an in-vivo colorectal cancer model Saudi Pharm J 2022-02-11 [PMID: 35145347] (WB) Details: Citation using the HRP version of this antibody.	WB
Marchini M, Ashkin MR, Bellini M Et al. A Na(+)/K(+) ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice Front Cell Dev Biol 2021-12-31 [PMID: 34970538] (IF/IHC) Details: Citation using the HRP version of this antibody.	IF/IHC
Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mech Ageing Dev 2021-10-26 [PMID: 34699858] (WB) Details: Citation using the HRP version of this antibody.	WB
Chen F, Liu J, Song X Et al. EZH2 inhibition confers PIK3CA-driven lung tumors enhanced sensitivity to PI3K inhibition Cancer Lett 2021-10-16 [PMID: 34655667] (WB) Details: Citation using the HRP version of this antibody.	WB
Markus F, Kannengieber A, NAder P Et al. A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects J Hum Genet 2021-07-02 [PMID: 34211111] Details: Citation using the HRP version of this antibody.
Abdel-Fattah MM, Elgendy ANAM, Mohamed WR. Xanthenone, ACE2 activator, counteracted gentamicin-induced nephrotoxicity in rats: Impact on oxidative stress and ACE2/Ang-(1-7) signaling Life Sci 2021-03-28 [PMID: 33774027] (WB) Details: Citation using the HRP format of this antibody.	WB
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (IHC-P) Details: Citation using the Alexa Fluor 488 version of this antibody.	IHC-P
Iguchi N, Carrasco A, Xie AX, et al Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways Sci Rep 2021-01-14 [PMID: 33441874] (WB) Details: Citation using the HRP version of this antibody.	WB
Ogura Y, Kakehashi C, Yoshihara T et al Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats PLoS One 2020-10-30 [PMID: 33125406] (WB) Details: Citation using the HRP version of this antibody.	WB
Harb I, Ashour H, Sabry D et al. Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1alpha/VEGF /eNOS signaling J. Pharm. Pharmacol. 2020-08-21 [PMID: 32822562] Details: Citation using the HRP format of this antibody.
Kaji S, Maki T, Ueda J et al. BCAS1-positive immature oligodendrocytes are affected by the alpha-synuclein-induced pathology of multiple system atrophy Acta Neuropathol Commun 2020-07-31 [PMID: 32727582] (WB) Details: This citation used the HRP format of this antibody.	WB
La Fuente JM, Sevilleja-Ortiz A, Garcia-Rojo E et al. Erectile dysfunction is associated with defective L-cysteine/hydrogen sulfide pathway in human corpus cavernosum and penile arteries Eur. J. Pharmacol. 2020-07-23 [PMID: 32712093] (WB) Details: Citation using the HRP format of this antibody.	WB
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun 2020-05-29 [PMID: 32472050] (WB) Details: Citation using the HRP format of this antibody.	WB
AbuBakr N, Haggag T, Sabry D et al. Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGF beta Smad3 signaling pathway Heliyon 2020-05-09 [PMID: 32382678] (WB)	WB
Gunasekar P, Satish M, Dabestani P et al. Modulation of Cathepsin L Expression in the Coronary Arteries of Atherosclerotic Swine J Surg Res 2019-08-05 [PMID: 31377485] (WB)	WB
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal 2019-07-06 [PMID: 31273575] (WB)	WB
Almalki SG, Llamas Valle Y, Agrawal DK. MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells Stem Cells Transl Med 2017-02-19 [PMID: 28213979] (WB)	WB
Hur J, Kim CS, Eo SK et al. Salmonella ghosts expressing enterotoxigenic Escherichia coli k88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model. Vet Q. [PMID: 25853619] (ELISA) Details: Citation using the HRP form of this antibody.	ELISA
Mehta NK Investigation of VISTA as a Cancer Immune Checkpoint Via Structural Analysis and Therapeutic Antibody Development Thesis 2020-01-01
Ganburged G, Suda N, Saito M et al. Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice Cell Tissue Res 2010-08-18 [PMID: 20714769] (IHC-P)	IHC-P
Chen F Epigenetic States Regulate Tumor Aggressiveness and Response to Targeted Therapies in Lung Adenocarcinoma Thesis 2021-01-01
Dickinson GH, Vega IE, Wahl KJ et al. Barnacle cement: a polymerization model based on evolutionary concepts. J Exp Biol. [PMID: 19837892] Details: Citation using the HRP form of this antibody.
Elmahmoudy B, Abd El Fattah M, Elyamany M et al. Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats Asian Pac J Trop Biomed 2019-10-01 (WB)	WB
W-M Fan T, Islam JMM, Higashi RM et al. Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions The Journal of biological chemistry 2023-11-20 [PMID: 37992808]
Chauhan NR, Kundu S, Bal R et al. Transgenic mouse models support a protective role of type I IFN response in SARS-CoV-2 infection-related lung immunopathology and neuroinvasion Cell reports 2023-10-23 [PMID: 37874678] (WB)	WB
Raafat RS, Habib MZ, AbdElfattah AA et al. Amisulpride attenuates 5-fluorouracil-induced cognitive deficits via modulating hippocampal Wnt/GSK-3?/?-catenin signaling in Wistar rats International immunopharmacology 2023-09-14 [PMID: 37716161] (WB)	WB
Silver B, Gerrish K, Tokar E. Cell-free DNA as a potential biomarker of differentiation and toxicity in cardiac organoids eLife 2023-06-01 [PMID: 37261896] (B/N)	B/N
Herrera-López EE, Guerrero-Escalera D, Aguirre-Maldonado I et al. Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis Scientific reports 2023-04-28 [PMID: 37117324] (WB)	WB
Ueda J, Uemura N, Ishimoto T et al. Ca2+ -Calmodulin-Calcineurin Signaling Modulates ?-Synuclein Transmission Movement disorders : official journal of the Movement Disorder Society 2023-04-17 [PMID: 37066491]
Elgamal M, Khodeer D, Abdel-Wahab B et al. Canagliflozin alleviates valproic acid-induced autism in rat pups: Role of PTEN/PDK/PPAR-? signaling pathways Frontiers in Pharmacology 2023-02-22 [PMID: 36909191] (WB)	WB
Khaled S, Abdelaziz RR, Suddek GM, Elshaer SL Candesartan protects against unilateral peripheral limb ischemia in type-2 diabetic rats: Possible contribution of PI3K-Akt-eNOS-VEGF angiogenic signaling pathway International immunopharmacology 2023-02-09 [PMID: 36773570] (WB)	WB
elkiki s, Galal s, gazzar E, Mansour H Ivermectin modulates lung toxicity induced by ?-radiation viaTLR4/ NF-?? /MAPK pathways Research Square 2023-05-22 (WB)	WB
Demers ND, Riccio V, Jo DS et al. PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS Autophagy 2023-06-01 [PMID: 36541703]
Sobhy N, Seif A, Abou-Bakr D et al. Potential Effect of Nigella sativa on Osteoporosis in Ovariectomized Rats: Possible Role of Hypoxia Inducible Factor 1-? Journal of Evolutionary Biochemistry and Physiology 2023-03-01
Angulo J, Fern�ndez A, Sevilleja-Ortiz A et al. Upregulation of Orai Channels Contributes to Aging-Related Vascular Alterations in Rat Coronary Arteries International Journal of Molecular Sciences 2023-08-29 [PMID: 37686206] (B/N)	B/N
Wright, KM;DiNapoli, SR;Miller, MS;Aitana Azurmendi, P;Zhao, X;Yu, Z;Chakrabarti, M;Shi, W;Douglass, J;Hwang, MS;Hsiue, EH;Mog, BJ;Pearlman, AH;Paul, S;Konig, MF;Pardoll, DM;Bettegowda, C;Papadopoulos, N;Kinzler, KW;Vogelstein, B;Zhou, S;Gabelli, SB; Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen Nature communications 2023-08-21 [PMID: 37604828]
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (WB)	WB
Zohny SM, Habib MZ, Mohamad MI et al. Memantine/Aripiprazole Combination Alleviates Cognitive Dysfunction in Valproic Acid Rat Model of Autism: Hippocampal CREB/BDNF Signaling and Glutamate Homeostasis Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2023-03-14 [PMID: 36918475] (WB)	WB
Chen F, Byrd AL, Liu J et al. Polycomb deficiency drives a FOXP2-high aggressive state targetable by epigenetic inhibitors Nature Communications 2023-01-20 [PMID: 36670102] (WB)	WB
El Assar M, GarcIa-Rojo E, Sevilleja-Ortiz A Et al. Functional Role of STIM-1 and Orai1 in Human Microvascular Aging Cells 2022-11-26 [PMID: 36429103] (WB) Details: Citation using the HRP version of this antibody.	WB
Acharya S, Anand S, Khan MA et al. Biphasic transcriptional and posttranscriptional regulation of MYB by androgen signaling mediates its growth control in prostate cancer Journal of Biological Chemistry 2023-01-01 [PMID: 36410437]
Tiwari R, Bommi PV, Gao P et al. Chemical inhibition of oxygen-sensing prolyl hydroxylases impairs angiogenic competence of human vascular endothelium through metabolic reprogramming iScience 2022-10-21 [PMID: 36157579] (Func)	Func
Colli LM, Jessop L, Myers TA Et al. Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus American journal of human genetics 2021-08-09 [PMID: 34390653] (B/N)	B/N
Padder RA, Bhat ZI, Ahmad Z et al. DRP1 Promotes BRAF(V600E)-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer Frontiers in Oncology 2021-03-02 [PMID: 33738242] (WB)	WB
D�az-Mart�n RD, Valencia-Hern�ndez JD, Betancourt-Lozano M, Y��ez-Rivera B. Changes in microtubule stability in zebrafish (Danio rerio) embryos after glyphosate exposure Heliyon 2021-01-21 [PMID: 33532646] (In Vivo)	In Vivo
Park SH, Lee DH, Choi HI et al. Synergistic lipid-lowering effects of Zingiber mioga and Hippophae rhamnoides extracts Experimental and Therapeutic Medicine 2020-06-19 [PMID: 32765704]
Mehta N, Maddineni S, Mathews II et al. Structure and Functional Binding Epitope of V-domain Ig Suppressor of T Cell Activation Cell Rep. 2019-09-03 [PMID: 31484064] (ELISA) Details: Citation used the HRP format of this antibody.	ELISA
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12 1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun. [PMID: 27384883] (WB) Details: Citation using the HRP form of this antibody.	WB
DuCote T Targeting EZH2 to Improve Outcomes of Lung Squamous Cell Carcinoma uknowledge.uky.edu 2023-01-01 (WB)	WB
Fujii S, Hara H, Araya J et al. Insufficient autophagy promotes bronchial epithelial cell senescence in chronic obstructive pulmonary disease. Oncoimmunology. [PMID: 22934255] Details: Citation using the HRP form of this antibody.
Lu Y, Kim C, Collins JF et al. Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes. J Trace Elem Med Biol. [PMID: 22579041] Details: Citation using the HRP form of this antibody.
Wang H, Kubica N, Ellisen LW et al. Dexamethasone represses signaling through the mammalian target of rapamycin in muscle cells by enhancing expression of REDD1. J Biol Chem. [PMID: 17074751] Details: Citation using the HRP form of this antibody.
Kimball SR, Siegfried BA, Jefferson LS et al. Glucagon represses signaling through the mammalian target of rapamycin in rat liver by activating AMP-activated protein kinase. J Biol Chem. [PMID: 15494402] Details: Citation using the HRP form of this antibody.
Roesler U, Scholz H, Hensel A et al. Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Int J Syst Evol Microbiol. [PMID: 12892149] Details: Citation using the HRP form of this antibody.
Show All 62 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	ELISA	Duck	02/14/2018	View Summary Application ELISA Sample Tested EDTA Plasma Species Duck Lot P40
Enlarge	5 reviewed by: Revathi Shanmugasundaram	ELISA	Turkey	02/06/2018	View Summary Application ELISA Sample Tested EDTA Plasma Species Turkey Lot P40
Enlarge	4 reviewed by: Verified Customer	WB	Yeast	12/05/2017	View Summary Application Western Blot Sample Tested p-38 Species Yeast Lot P40
Enlarge	5 reviewed by: Verified Customer	WB	Human	09/25/2014	View Summary Application Western Blot Sample Tested THP1 cells Species Human

Array NB7160

• IgG (H+L) Antibodies