![Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Western-Blot-NBP2-67321-img0010.jpg "Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SR41-02 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human SHP-1 aa 537-595 / 595. (SwissProt: P29350 Human; SwissProt: P29351 Mouse; SwissProt: P81718 Rat) |
| Localization | Cytoplasm, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PTPN6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP assay-dependent |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot SHIP Antibody (257812) [Unconjugated]](https://images.novusbio.com/images2/SHIP_MAB2317_Western_Blot_6027.jpg)
![Immunocytochemistry SHIP Antibody (257812) [Unconjugated]](https://images.novusbio.com/images2/SHIP_MAB2317_Immunocytochemistry_9852.jpg)
![Knockout Validated SHIP Antibody (257812) [Unconjugated]](https://images.novusbio.com/images/mab2317_human-mouse-rat-ship-mab-clone-257812-20820241518568.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for SHP-1 Antibody (NBP2-67321)Find related products by research area.
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| Gene Symbol | PTPN6 |
![Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunocytochemistry-Immunofluorescence-NBP2-67321-img0003.jpg "Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0011.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m")
![Flow Cytometry: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Flow-Cytometry-NBP2-67321-img0001.jpg "Flow Cytometry: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunocytochemistry-Immunofluorescence-NBP2-67321-img0002.jpg "Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded human tonsil tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0004.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded human tonsil tissue using anti-SHP1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0005.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse liver tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0006.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse liver tissue using anti-SHP1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse spleen tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0007.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse spleen tissue using anti-SHP1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded rat brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0008.jpg "Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded rat brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.")
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![Simple Western SHP-2/PTPN11 [p Tyr542] Antibody [Unconjugated]](https://images.novusbio.com/images2/16330.jpg)
![Immunocytochemistry SHP-2/PTPN11 [p Tyr542] Antibody [Unconjugated]](https://images.novusbio.com/images2/af3790_human-mouse-phospho-shp-2-y542-affinity-purified-pab-immunocytochemistry-243202212955.jpg)
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Intracellular Staining by Flow Cytometry Reg1A Antibody (431202) [Unconjugated]](https://images.novusbio.com/images2/Reg1A_MAB4937_Flow_Cytometry_8512.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Simple_Western_21000.jpg)
![Western Blot STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Western_Blot_5993.jpg)
![Knockout Validated STAT3 Antibody (232209) [Unconjugated]](https://images.novusbio.com/images2/STAT3_MAB1799_Knockout_Validated_21531.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot GRB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GRB2_AF3846_Western_Blot_5355.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")