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SHIP Antibody (257812) [Unconjugated]

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Western blot shows lysates of TF-1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for SHIP was detected at approximately 148 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of TF-1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP ...read more
SHIP was detected in immersion fixed mouse splenocytes using Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
Genetic Strategies: U-937 WT and SHIP KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KO
Clone
257812
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2317-SP
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SHIP Antibody (257812) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse SHIP
Glu874-Thr941
Accession # Q9ES52
Specificity
Detects human, mouse, and rat SHIP in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
INPP5D
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHIP Antibody (257812) [Unconjugated]

  • 145kD
  • EC 3.1.3
  • hp51CNMGC142142
  • Inositol polyphosphate-5-phosphatase of 145 kDa
  • inositol polyphosphate-5-phosphatase, 145kDa
  • INPP5D
  • MGC104855
  • SHIP
  • SHIPMGC142140

Background

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol INPP5D
Uniprot