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Rat IgG2a Isotype Control (54447) [Unconjugated]

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B16-F1 mouse melanoma cell line was stained with RatAnti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405,filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB006,open histogram), ...read more
CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Ctrl
Clone
54447
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Rat IgG2a Isotype Control (54447) [Unconjugated] Summary

Immunogen
KLH
Specificity
Selected for use as rat IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha , rrCINC-2 beta , rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhEotaxin-3, rmEotaxin 2, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin -1, rhLymphytactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha , rmMIP-1 alpha , rmMIP-1 beta , rmMIP-1 gamma , rhMIP-1δ, rmMIP-2, rhMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , rmMIP-3 alpha , rmMIP-3 beta , rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha , rmSDF-1 alpha , rhSDF-1 beta , rhTarc, rmTARC, rmTCA-3, rhTeck, or rmTeck.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Dilutions
  • Control
Reviewed Applications
Read 1 Review rated 5
using
MAB006 in the following application:

Publications
Read Publications using
MAB006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2a Isotype Control (54447) [Unconjugated]

  • IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (MAB006)(210)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 16 applications: Bioassay, Bioassay Control, Control, ELISA Development, Flow Cytometry, Flow Cytometry Control, Functional Assay, IHC, IHC Control, IHC-Fr, IP, In Vivo, In Vivo Control, Isotype Control, Neutralization, Neutralization Control.


Filter By Application
Bioassay
(1)
Bioassay Control
(1)
Control
(47)
ELISA Development
(1)
Flow Cytometry
(10)
Flow Cytometry Control
(2)
Functional Assay
(1)
IHC
(4)
IHC Control
(1)
IHC-Fr
(2)
IP
(1)
In Vivo
(12)
In Vivo Control
(4)
Isotype Control
(12)
Neutralization
(28)
Neutralization Control
(13)
All Applications
Filter By Species
Human
(11)
Mouse
(126)
Rat
(4)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 210. Show All 210 Publications.
Publications using MAB006 Applications Species
Rodríguez-Míguez, Y;Lozano-Ordaz, V;Ortiz-Cabrera, AE;Barrios-Payan, J;Mata-Espinosa, D;Huerta-Yepez, S;Baay-Guzman, G;Hernández-Pando, R; Effect of IL-17A on the immune response to pulmonary tuberculosis induced by high- and low-virulence strains of Mycobacterium bovis PloS one 2024-07-18 [PMID: 39024223] (Neutralization, Mouse) Neutralization Mouse
Díez-Sánchez, A;Lindholm, HT;Vornewald, PM;Ostrop, J;Yao, R;Single, AB;Marstad, A;Parmar, N;Shaw, TN;Martín-Alonso, M;Oudhoff, MJ; LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model Nature communications 2024-04-22 [PMID: 38649356] (Neutralization Control, Transgenic Mouse) Neutralization Control Transgenic Mouse
Uddin, MJ;Thompson, B;Leslie, JL;Fishman, C;Sol-Church, K;Kumar, P;Petri, WA; Investigating the impact of antibiotic-induced dysbiosis on protection from Clostridium difficile colitis by mouse colonic innate lymphoid cells mBio 2024-02-20 [PMID: 38376154] (In Vivo, Mouse) In Vivo Mouse
O'Keefe, RN;Carli, ALE;Baloyan, D;Chisanga, D;Shi, W;Afshar-Sterle, S;Eissmann, MF;Poh, AR;Pal, B;Seillet, C;Locksley, RM;Ernst, M;Buchert, M; A tuft cell - ILC2 signaling circuit provides therapeutic targets to inhibit gastric metaplasia and tumor development Nature communications 2023-10-28 [PMID: 37898600] (Neutralization Control, In Vivo Control, Mouse) Neutralization Control, In Vivo Control Mouse
Cheng, M;Zheng, Y;Fan, Y;Yan, P;Zhao, W; The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies International immunopharmacology 2023-09-16 [PMID: 37722259] (Neutralization Control, Mouse) Neutralization Control Mouse
Wei, C;Datta, PK;Siegerist, F;Li, J;Yashwanth, S;Koh, KH;Kriho, NW;Ismail, A;Luo, S;Fischer, T;Amber, KT;Cimbaluk, D;Landay, A;Endlich, N;Rappaport, J;Michigan Medicine COVID−19 Investigators, ;Hayek, SS;Reiser, J; SuPAR mediates viral response proteinuria by rapidly changing podocyte function Nature communications 7/21/2023 [PMID: 37479685]
Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF; IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils Cell reports 2023-06-08 [PMID: 37300834] (In Vivo, Mouse) In Vivo Mouse
Ayyaswamy, S;Shi, H;Zhang, B;Bryan, RM;Durgan, DJ; Obstructive Sleep Apnea-Induced Hypertension Is Associated With Increased Gut and Neuroinflammation Journal of the American Heart Association 2023-06-06 [PMID: 37260032] (In Vivo, Rat) In Vivo Rat
Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH; Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation Mucosal immunology 2023-05-10 [PMID: 37172907] (In Vivo Control, Mouse) In Vivo Control Mouse
BJ Choi, MH Park, KH Park, WH Han, HJ Yoon, HY Jung, JY Hong, MR Chowdhury, KY Kim, J Lee, IS Song, M Pang, MK Choi, E Gulbins, M Reichel, J Kornhuber, CW Hong, C Kim, SH Kim, EH Schuchman, HK Jin, JS Bae Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer&#039;s disease Nature Communications, 2023-03-24;14(1):1631. 2023-03-24 [PMID: 36959217] (Isotype Control, Mouse) Isotype Control Mouse
Show All 210 Publications.

Review for IgG2a Isotype Control (MAB006) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using MAB006:
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 IgG2a MAB006
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Verified Customer
06/13/2017
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Summary

LotCA02315081

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