Home » STAT3 » STAT3 Antibodies » STAT3 Antibody (232209) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

STAT3 Antibody (232209) [Unconjugated]

 (22 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell ...read more
STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells ...read more
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more
HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG ...read more
Genetic Strategies: STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1, but is not detected in STAT3 knockout (KO) HeLa cell line using Mouse ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa ...read more
Genetic Strategies: STAT3 knockout HeLa human cervical epithelial cell line was stained with Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP, Flow, ICC/IF, KO
Clone
232209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1799U
Alexa Fluor 350
IC1799U-100UG
Alexa Fluor 405
FAB1799V
Alexa Fluor 405
IC1799V-100UG
Alexa Fluor 488
IC1799G-100UG
Alexa Fluor 488
FAB1799G
Alexa Fluor 532
FAB1799X
Alexa Fluor 594
FAB1799T
Alexa Fluor 594
IC1799T-100UG
Alexa Fluor 647
IC1799R-100UG
Alexa Fluor 647
FAB1799R
Alexa Fluor 700
IC1799N-100UG
Alexa Fluor 700
FAB1799N
Alexa Fluor 750
FAB1799S
Alexa Fluor 750
IC1799S-100UG
Allophycocyanin
FAB1799A
Biotin
FAB1799B
DyLight 350
FAB1799D
DyLight 405
FAB1799E
DyLight 488
FAB1799K
DyLight 550
FAB1799L
DyLight 594
FAB1799M
DyLight 650
FAB1799W
DyLight 680
FAB1799Y
DyLight 755
FAB1799Z
Fluorescein
IC1799F
HRP
FAB1799H
Janelia Fluor 549
FAB1799I
Janelia Fluor 646
FAB1799J
PerCP
FAB1799C
Phycoerythrin
IC1799P
mFluor Violet 450 SE
FAB1799MFV450
mFluor Violet 500 SE
FAB1799MFV500
mFluor Violet 610 SE
FAB1799MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC1799P
Carrier Free
IC1799F
Carrier Free
MAB1799-SP
Carrier Free
IC1799V-100UG
Carrier Free
IC1799U-100UG
Carrier Free
IC1799T-100UG
Carrier Free
IC1799S-100UG
Carrier Free
IC1799R-100UG
Carrier Free
IC1799N-100UG
Carrier Free
IC1799G-100UG
100% Guarantee on every product

STAT3 Antibody (232209) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human STAT3
Met1-Asn175
Accession # P40763
Specificity
Detects human, mouse, and rat STAT3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STAT3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunoprecipitation 1-3 ug/500 ug cell lysate
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB1799 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT3 Antibody (232209) [Unconjugated]

  • Acute-phase response factor
  • APRFMGC16063
  • DNA-binding protein APRF
  • FLJ20882
  • HIES
  • signal transducer and activator of transcription 3 (acute-phase responsefactor)
  • signal transducer and activator of transcription 3
  • STAT3

Background

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

STAT1 [p Tyr701]
Antibody
AF2894
Western Blot STAT1 [p Tyr701] Antibody
Simple Western STAT1 [p Tyr701] Antibody
Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
Jak2 Antibody
(53B7) - BSA Free
NBP2-59451
Flow Cytometry Jak2 Antibody (53B7) - BSA Free
Immunocytochemistry/ Immunofluorescence Jak2 Antibody (53B7) - BSA Free
Western Blot Jak2 Antibody (53B7) - BSA Free
(3 Reviews)
(2 Publications)
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
SOAT1 Antibody
NB400-141
SOAT1 Antibody
(7 Publications)
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
STAT5a/b Antibody
[Unconjugated] - Pan ...
AF2168
Western Blot STAT5a/b Antibody [Unconjugated] - Pan Specific
Simple Western STAT5a/b Antibody [Unconjugated] - Pan Specific
Chromatin Immunoprecipitation (ChIP) STAT5a/b Antibody [Unconjugated] - Pan Specific
(28 Publications)
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
STAT5b Antibody
[Unconjugated]
AF1584
Western Blot STAT5b Antibody [Unconjugated]
Simple Western STAT5b Antibody [Unconjugated]
Knockout Validated STAT5b Antibody [Unconjugated]
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(287 Publications)
Species: Mu
Applications: ELISA
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
LIF [Unconjugated]
7734-LF
LIF [Unconjugated]
(29 Publications)
Species: Hu
Applications: BA
gp130/CD130 [HRP]
DGP00
N/A gp130/CD130 [HRP]
N/A gp130/CD130 [HRP]
N/A gp130/CD130 [HRP]
(14 Publications)
Species: Hu
Applications: ELISA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(672 Publications)
Species: Hu
Applications: ELISA
Jak1 Antibody
(413104) [Unconjugate
MAB4260
Western Blot Jak1 Antibody (413104) [Unconjugated]
Immunohistochemistry Jak1 Antibody (413104) [Unconjugated]
Intracellular Staining by Flow Cytometry Jak1 Antibody (413104) [Unconjugated]
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
SOCS-3 Antibody
(516919) [Unconjugate
MAB5696
Western Blot SOCS-3 Antibody (516919) [Unconjugated]
(6 Publications)
Species: Hu, Mu
Applications: WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(62 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Leptin/OB [HRP]
DLP00
N/A Leptin/OB [HRP]
N/A Leptin/OB [HRP]
(150 Publications)
Species: Hu
Applications: ELISA
Bcl-xL Antibody
NB100-56104
Western Blot Bcl-xL Antibody
Immunohistochemistry-Paraffin Bcl-xL Antibody
Western Blot Bcl-xL Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
STAT3 Antibody
(232209) [Unconjugate
MAB1799
STAT3 Antibody (232209) [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, IP, Flow, ICC/IF, KO

Publications for STAT3 Antibody (MAB1799)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Functional Assay, Western Blot.


Filter By Application
Functional Assay
(1)
Western Blot
(12)
All Applications
Filter By Species
Human
(10)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB1799 Applications Species
Kim, J;Ham, J;Kang, HR;Bae, YS;Kim, T;Kim, HY; JAK3 inhibitor suppresses multipotent ILC2s and attenuates steroid-resistant asthma Science advances 2023-12-22 [PMID: 38117887] (Functional Assay, Mouse) Functional Assay Mouse
S Li, DM Pritchard, LG Yu Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death & Disease, 2023-04-13;14(4):268. 2023-04-13 [PMID: 37055381] (Western Blot, Human) Western Blot Human
Kensuke Iwasa, Anzu Yamagishi, Shinji Yamamoto, Chikara Haruta, Kei Maruyama, Keisuke Yoshikawa GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells Neurochemical Research 3/1/2023 [PMID: 36436172]
F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 2022-10-01;21(12):103207. 2022-10-01 [PMID: 36191778] (Western Blot, Human) Western Blot Human
Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 9/8/2022 [PMID: 36145344]
JC Kershaw, BD Elzey, XX Guo, KH Kim Piceatannol, a Dietary Polyphenol, Alleviates Adipose Tissue Loss in Pre-Clinical Model of Cancer-Associated Cachexia via Lipolysis Inhibition Nutrients, 2022-05-31;14(11):. 2022-05-31 [PMID: 35684106] (Western Blot, Mouse) Western Blot Mouse
Justin Harper, Susan P. Ribeiro, Chi Ngai Chan, Malika Aid, Claire Deleage, Luca Micci et al. Interleukin-10 contributes to reservoir establishment and persistence in SIV-infected macaques treated with antiretroviral therapy Journal of Clinical Investigation 4/15/2022 [PMID: 35230978]
R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-11-22;22(22):. 2021-11-22 [PMID: 34830441] (Western Blot, Human) Western Blot Human
R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-08-12;22(16):. 2021-08-12 [PMID: 34445392] (Western Blot, Human) Western Blot Human
Huairong Zhang, Andrea Liliam Gomez Corredor, Julia Messina-Pacheco, Qing Li, George Zogopoulos, Nancy Kaddour et al. REG3A/REG3B promotes acinar to ductal metaplasia through binding to EXTL3 and activating the RAS-RAF-MEK-ERK signaling pathway Communications Biology 6/7/2021 [PMID: 34099862]
Show All 22 Publications.

Reviews for STAT3 Antibody (MAB1799) (0)

There are no reviews for STAT3 Antibody (MAB1799). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAT3 Antibody (MAB1799) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional STAT3 Products

Blogs on STAT3.

Check out the latest blog posts on STAT3.
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.
KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.
HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.
HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.
TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.
Thymoquinone: A Natural Product with Diverse Therapeutic Potential
Thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone, or TQ) is derived from the seeds of the black cumin plant Nigella sativa. It has been reported to have a number of beneficial properties including anti-oxidative, anti-inflammatory and anti-tumorige...  Read full blog post.
Survivin Acetylation: Affecting Apoptosis and Cancer
Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STAT3 Antibody (232209) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol STAT3
Uniprot