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STAT3 Antibody (232209) [Unconjugated]

 (23 Publications)
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Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NCI‑H1703 human non-small cell lung carcinoma cell line, C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT3 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NCI‑H1703 human non-small cell lung carcinoma cell line, C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell ...read more
STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells ...read more
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more
HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG ...read more
Genetic Strategies: STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1, but is not detected in STAT3 knockout (KO) HeLa cell line using Mouse ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa ...read more
Genetic Strategies: STAT3 knockout HeLa human cervical epithelial cell line was stained with Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP, Flow, ICC/IF, KO
Clone
232209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1799U-100UG
Alexa Fluor 405
IC1799V-100UG
Alexa Fluor 488
IC1799G-100UG
Alexa Fluor 532
FAB1799X
Alexa Fluor 594
IC1799T-100UG
Alexa Fluor 647
IC1799R-100UG
Alexa Fluor 700
IC1799N-100UG
Alexa Fluor 750
IC1799S-100UG
Allophycocyanin
FAB1799A
Biotin
FAB1799B
CoraFluor 1
FAB1799CL1
DyLight 350
FAB1799D
DyLight 405
FAB1799E
DyLight 488
FAB1799K
DyLight 550
FAB1799L
DyLight 594
FAB1799M
DyLight 650
FAB1799W
DyLight 680
FAB1799Y
DyLight 755
FAB1799Z
Fluorescein
IC1799F
HRP
FAB1799H
Janelia Fluor 525
FAB1799JF525
Janelia Fluor 549
FAB1799I
Janelia Fluor 646
FAB1799J
Janelia Fluor 669
FAB1799JF669
PerCP
FAB1799C
Phycoerythrin
IC1799P
mFluor Violet 450 SE
FAB1799MFV450
mFluor Violet 500 SE
FAB1799MFV500
mFluor Violet 610 SE
FAB1799MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC1799P
Carrier Free
IC1799F
Carrier Free
MAB1799-SP
Carrier Free
IC1799V-100UG
Carrier Free
IC1799U-100UG
Carrier Free
IC1799T-100UG
Carrier Free
IC1799S-100UG
Carrier Free
IC1799R-100UG
Carrier Free
IC1799N-100UG
Carrier Free
IC1799G-100UG
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STAT3 Antibody (232209) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human STAT3
Met1-Asn175
Accession # P40763
Specificity
Detects human, mouse, and rat STAT3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STAT3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunoprecipitation 1-3 ug/500 ug cell lysate
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1799 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT3 Antibody (232209) [Unconjugated]

  • Acute-phase response factor
  • APRFMGC16063
  • DNA-binding protein APRF
  • FLJ20882
  • HIES
  • signal transducer and activator of transcription 3 (acute-phase responsefactor)
  • signal transducer and activator of transcription 3
  • STAT3

Background

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT3 Antibody (MAB1799)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Co-Immunoprecipitation, Functional Assay, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
Functional Assay
(1)
Western Blot
(12)
All Applications
Filter By Species
Human
(11)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using MAB1799 Applications Species
Chakraborty, R;Khodlan, P;Tay, A;Liu, F; STAT3 interactome predicts presence of proteins that regulates immune system in oral squamous cell carcinoma Journal of oral biosciences 2024-09-03 [PMID: 39237001] (Co-Immunoprecipitation, Human) Co-Immunoprecipitation Human
Kim, J;Ham, J;Kang, HR;Bae, YS;Kim, T;Kim, HY; JAK3 inhibitor suppresses multipotent ILC2s and attenuates steroid-resistant asthma Science advances 2023-12-22 [PMID: 38117887] (Functional Assay, Mouse) Functional Assay Mouse
S Li, DM Pritchard, LG Yu Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death & Disease, 2023-04-13;14(4):268. 2023-04-13 [PMID: 37055381] (Western Blot, Human) Western Blot Human
Kensuke Iwasa, Anzu Yamagishi, Shinji Yamamoto, Chikara Haruta, Kei Maruyama, Keisuke Yoshikawa GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells Neurochemical Research 3/1/2023 [PMID: 36436172]
F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 2022-10-01;21(12):103207. 2022-10-01 [PMID: 36191778] (Western Blot, Human) Western Blot Human
Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 9/8/2022 [PMID: 36145344]
JC Kershaw, BD Elzey, XX Guo, KH Kim Piceatannol, a Dietary Polyphenol, Alleviates Adipose Tissue Loss in Pre-Clinical Model of Cancer-Associated Cachexia via Lipolysis Inhibition Nutrients, 2022-05-31;14(11):. 2022-05-31 [PMID: 35684106] (Western Blot, Mouse) Western Blot Mouse
Justin Harper, Susan P. Ribeiro, Chi Ngai Chan, Malika Aid, Claire Deleage, Luca Micci et al. Interleukin-10 contributes to reservoir establishment and persistence in SIV-infected macaques treated with antiretroviral therapy Journal of Clinical Investigation 4/15/2022 [PMID: 35230978]
R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-11-22;22(22):. 2021-11-22 [PMID: 34830441] (Western Blot, Human) Western Blot Human
R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-08-12;22(16):. 2021-08-12 [PMID: 34445392] (Western Blot, Human) Western Blot Human
Show All 23 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

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FAQs for STAT3 Antibody (MAB1799) (0)

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Secondary Antibodies

 

Isotype Controls

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Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.
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Bioinformatics

Gene Symbol STAT3
Uniprot