STAT3 Antibodies
We offer STAT3 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each STAT3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our STAT3 Antibodies can be used in a variety of model species: Canine, Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the STAT3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our STAT3 polyclonal antibodies and browse our STAT3 monoclonal antibody catalog.
Alternate Names for STAT3 Antibodies
anti-STAT3 antibody, anti-Acute-phase response factor antibody, anti-APRFMGC16063 antibody, anti-DNA-binding protein APRF antibody, anti-FLJ20882 antibody, anti-HIES antibody, anti-signal transducer and activator of transcription 3 (acute-phase responsefactor) antibody, anti-signal transducer and activator of transcription 3 antibody
22 Results for "stat3" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: STAT3 Antibody [NB100-799] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and weak nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/STAT3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-799-img0005.jpg "Immunocytochemistry/Immunofluorescence: STAT3 Antibody [NB100-799] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and weak nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/STAT3-Antibody-Immunohistochemistry-Paraffin-NB100-799-img0006.jpg)
![Flow Cytometry: STAT3 Antibody [NB100-799] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-Flow-Cytometry-NB100-799-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Staining of Mouse Thymus. Antibody at 4 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/STAT3-Antibody-Immunohistochemistry-Paraffin-NB100-799-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Negative Control showing staining of Mouse Thymus, with no primary antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-Immunohistochemistry-Paraffin-NB100-799-img0004.jpg)
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From verified customer review.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Western-Blot-NBP2-22471-img0023.jpg "Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From verified customer review.")
![Immunocytochemistry/Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Immunocytochemistry-Immunofluorescence-NBP2-22471-img0016.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (9D8) [NBP2-22471] - Biopsies of normal and cancer tissues.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Immunohistochemistry-Paraffin-NBP2-22471-img0017.jpg)
![Chromatin Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay with STAT3 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of LAMC1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown; boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Chromatin-Immunoprecipitation-ChIP-NBP2-22471-img0020.jpg)
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug HepG2 total lysate.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Western-Blot-NBP2-22471-img0010.jpg)
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of various whole cell lysates.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Western-Blot-NBP2-22471-img0019.jpg)
![Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of U2-OS lysate from STAT3 SMART pool siRNA transfected or non-targeting control transfected U2-OS cells onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Western-Blot-NBP2-22471-img0022.jpg)
![Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 was performed on HepG2 cells. The antigen: antibody complex was formed by incubating 750ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing STAT3 overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing STAT3 at a dilution of 1:5000 overnight rotating at 4C. Membranes were washed in TBST and probed with Clean-blot IP detection reagent at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.](http://images.novusbio.com/fullsize/STAT3-Antibody-9D8-Immunoprecipitation-NBP2-22471-img0021.jpg)
![Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of phospho-Stat3 was detected. Image from verified customer review.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NBP2-24463-img0003.jpg "Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of phospho-Stat3 was detected. Image from verified customer review.")
![Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Analysis of STAT3 (Tyr705) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 705 and 2) an unphosphorylated tyrosine at position 705, using STAT3 phospho antibody at 0.1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NBP2-24463-img0001.jpg)
![Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer cells MDA-MB-231 were treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected. Image from verified customer review.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NBP2-24463-img0002.jpg)
![Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213] - Analysis of extracts from Hela cells untreated or treated with IFN a using STAT3](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Western-Blot-NB100-82213-img0001.jpg "Western Blot: STAT3 [p Tyr705] Antibody [NB100-82213] - Analysis of extracts from Hela cells untreated or treated with IFN a using STAT3")
![Immunocytochemistry/Immunofluorescence: STAT3 [p Tyr705] Antibody [NB100-82213] - Sstaining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82213-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of STAT3 (Tyr705) in paraffin-embedded human breast carcinoma tissue using NB100-82213.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Immunohistochemistry-Paraffin-NB100-82213-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Immunohistochemistry-Paraffin-NB100-82213-img0006.jpg)
![Simple Western: STAT3 [p Tyr705] Antibody [NB100-82213] - STAT3-P assay on mouse megakaryocyte lysates. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/STAT3-[p-Tyr705]-Antibody-Simple-Western-NB100-82213-img0008.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.](http://images.novusbio.com/fullsize/nb100-82213_rabbit-polyclonal-stat3-p-tyr705-antibody-23420241446223.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.](http://images.novusbio.com/fullsize/nb100-82213_rabbit-polyclonal-stat3-p-tyr705-antibody-23420241446229.jpg)
![Immunocytochemistry/ Immunofluorescence: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunofluorescence analysis of mouse-spleen tissue.](http://images.novusbio.com/fullsize/nb100-82213_rabbit-polyclonal-stat3-p-tyr705-antibody-23420241446222.png)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0010.jpg "Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of STAT3 [p Ser727] on NIH-3T3 cell lysates. Lane 1: NIH-3T3 cells, whole cell lysates, 10 ug/lane. Lane 2: NIH-3T3 cells were starved 6h, whole cell lysates, 10 ug/lane. Lane 3/4: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, whole cell lysates, 10 ug/lane. Lane 5: NIH-3T3 cells were starved 6h, then treated with 200 ng/ml EGF for 10 min, and then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10 ug/lane. The primary antibody Anti-STAT3 [p Ser727] (1/500) , Anti-STAT3 antibody ( 1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 88 kDa Observed band size: 88 kDa")
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0014.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0008.jpg)
![Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Human U251 glioma cell line, whole cell lysates.Cells were treated with SP600125, DB07268, JNK-IN-8 and SB203580. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Western-Blot-NBP2-67433-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunocytochemistry-Immunofluorescence-NBP2-67433-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0012.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-p-Ser727-Antibody-SY24-09-Immunohistochemistry-Paraffin-NBP2-67433-img0013.jpg)
![Western Blot: STAT3 Antibody (SY24-08) [NBP2-67432] - Western blot analysis of STAT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Western-Blot-NBP2-67432-img0009.jpg "Western Blot: STAT3 Antibody (SY24-08) [NBP2-67432] - Western blot analysis of STAT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunocytochemistry/Immunofluorescence: STAT3 Antibody (SY24-08) [NBP2-67432] - Staining Stat3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunocytochemistry-Immunofluorescence-NBP2-67432-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-STAT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-38, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0008.jpg)
![Western Blot: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of Stat3 on different lysates using anti-Stat3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: SHG-44 Lane 3: SW480](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Western-Blot-NBP2-67432-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded human colon cancer tissue using anti-Stat3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0001.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded human pancreas tissue using anti-Stat3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded mouse brain tissue using anti-Stat3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Stat3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-STAT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/STAT3-Antibody-SY24-08-Immunohistochemistry-Paraffin-NBP2-67432-img0007.jpg)
![Western Blot: STAT3 Antibody (CL0492) [NBP2-52936] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Western-Blot-NBP2-52936-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: STAT3 Antibody (CL0492) [NBP2-52936] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of A549 cells using the Anti-STAT3 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunocytochemistry-Immunofluorescence-NBP2-52936-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining in human endometrium and pancreas tissues. Corresponding STAT3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunohistochemistry-Paraffin-NBP2-52936-img0014.jpg)
![Western Blot: STAT3 Antibody (CL0492) [NBP2-52936] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Western-Blot-NBP2-52936-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human endometrium shows strong nuclear and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunohistochemistry-Paraffin-NBP2-52936-img0008.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunohistochemistry-Paraffin-NBP2-52936-img0009.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human rectum shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunohistochemistry-Paraffin-NBP2-52936-img0010.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/STAT3-Antibody-CL0492-Immunohistochemistry-Paraffin-NBP2-52936-img0011.jpg)
![Western Blot: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using NBP2-61588, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.](http://images.novusbio.com/fullsize/STAT3-p-Tyr705-Antibody-RM261-Western-Blot-NBP2-61588-img0006.jpg "Western Blot: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using NBP2-61588, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.")
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61588.](http://images.novusbio.com/fullsize/STAT3-p-Tyr705-Antibody-RM261-Immunohistochemistry-Paraffin-NBP2-61588-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody (RM261) [NBP2-61588] - Human intestine. Image from verified customer review.](http://images.novusbio.com/fullsize/STAT3-p-Tyr705-Antibody-RM261-Immunohistochemistry-Paraffin-NBP2-61588-img0005.jpg)
![Knockout Validated: STAT3 Antibody (OTI21E7) [NBP1-48014] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and STAT3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.(1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: STAT3 Antibody (OTI21E7) [NBP1-48014] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and STAT3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.(1:500)")
![<b>Genetic Strategies Validation. </b>Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] Staining of paraffin-embedded thyroid using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0009.jpg)
![Western Blot: STAT3 Antibody (21E7) [NBP1-48014] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Western-Blot-NBP1-48014-img0001.jpg)
![Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0010.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded breast using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of lung using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded colon using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded kidney using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded lung using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0008.jpg)