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STAT1 [p Tyr701] Antibody [Unconjugated]

 (11 Publications)
 (1 Review)
 
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse ...read more
Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF2894-SP
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STAT1 [p Tyr701] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human STAT1 Y701 site
Modification
p Tyr701
Specificity
Detects human STAT1 when phosphorylated at Y701 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
AF2894 in the following applications:
Publications
Read Publications using
AF2894 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT1 [p Tyr701] Antibody [Unconjugated]

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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STAT1 [p Tyr701]
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AF2894
STAT1 [p Tyr701] Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, Flow

Publications for STAT1 Antibody (AF2894)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 4 applications: Capillary Immunoblot, ICC, IHC, Western Blot.


Filter By Application
Capillary Immunoblot
(1)
ICC
(1)
IHC
(2)
Western Blot
(5)
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Human
(3)
Mouse
(5)
Hamster
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2894 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Weilun Pang, Fenghua Hu C9ORF72 suppresses JAK-STAT mediated inflammation iScience 5/19/2023 [PMID: 37250330]
G Coquant, D Aguanno, L Brot, C Belloir, J Delugeard, N Roger, HP Pham, L Briand, M Moreau, L de Sordi, V Carrière, JP Grill, S Thenet, P Seksik 3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors Oncogene, 2022-06-08;12(1):9440. 2022-06-08 [PMID: 35676403] (Capillary Immunoblot, Mouse) Capillary Immunoblot Mouse
SGPJ Korsten, L Peracic, LMB van Groeni, MAP Diks, H Vromans, J Garssen, LEM Willemsen Butyrate Prevents Induction of CXCL10 and Non-Canonical IRF9 Expression by Activated Human Intestinal Epithelial Cells via HDAC Inhibition International Journal of Molecular Sciences, 2022-04-02;23(7):. 2022-04-02 [PMID: 35409339] (Western Blot, Human) Western Blot Human
KT Tang, CC Lin, SC Lin, JH Wang, SW Tsai Kurarinone Attenuates Collagen-Induced Arthritis in Mice by Inhibiting Th1/Th17 Cell Responses and Oxidative Stress International Journal of Molecular Sciences, 2021-04-13;22(8):. 2021-04-13 [PMID: 33924467] (Western Blot, Mouse) Western Blot Mouse
PD-L1 blockade enhances response of pancreatic ductal adenocarcinoma to radiotherapy EMBO Mol Med, 2017-02-01;0(0):. 2017-02-01 [PMID: 27932443] (Western Blot, Mouse) Western Blot Mouse
Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes Sci Rep, 2016-07-29;6(0):30448. 2016-07-29 [PMID: 27469302] (Western Blot, Mouse) Western Blot Mouse
Molecular determinants of plaque size as an indicator of dengue virus attenuation Sci Rep, 2016-05-17;6(0):26100. 2016-05-17 [PMID: 27185466] (IHC, Hamster) IHC Hamster
Berri F, Haffar G, Le V, Sadewasser A, Paki K, Lina B, Wolff T, Riteau B Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication. J Virol, 2014-07-16;88(19):11215-28. 2014-07-16 [PMID: 25031344] (Western Blot, Human) Western Blot Human
Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al. IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism Angiogenesis 12/1/2012 [PMID: 22797886]
Show All 11 Publications.

Review for STAT1 Antibody (AF2894) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2894:
Filter by Applications
WB
(1)
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Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot STAT1 AF2894
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 04/04/2020
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Summary

ApplicationWestern Blot
Sample TestedHuman lung macrophages,Jurkat
SpeciesHuman
LotVXC0418041

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Recent Reviews

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Western Blot STAT1 AF2894
Enlarge
 Verified Customer
04/04/2020
Application: WB
Species: Human
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Bioinformatics

Gene Symbol STAT1