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STAT2 [p Tyr689] Antibody (1021D) [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit ...read more
Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1, filled histogram) for 20 minutes was stained with Rabbit Anti-Human Phospho-STAT2 (Y690) ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was ...read more
Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow
Clone
1021D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2890U
Alexa Fluor 350
IC2890U-100UG
Alexa Fluor 405
FAB2890V
Alexa Fluor 405
IC2890V-100UG
Alexa Fluor 488
IC8627G-025
Alexa Fluor 488
FAB2890G
Alexa Fluor 532
FAB2890X
Alexa Fluor 594
FAB2890T
Alexa Fluor 594
IC2890T-100UG
Alexa Fluor 647
IC2890R-100UG
Alexa Fluor 647
FAB2890R
Alexa Fluor 700
IC2890N-100UG
Alexa Fluor 700
FAB2890N
Alexa Fluor 750
IC2890S-100UG
Alexa Fluor 750
FAB2890S
Allophycocyanin
FAB2890A
Allophycocyanin/Cy7
FAB2890APCCY7
Biotin
FAB2890B
DyLight 350
FAB2890D
DyLight 405
FAB2890E
DyLight 488
FAB2890K
DyLight 550
FAB2890L
DyLight 594
FAB2890M
DyLight 650
FAB2890W
DyLight 680
FAB2890Y
DyLight 755
FAB2890Z
FITC
FAB2890F
HRP
FAB2890H
Janelia Fluor 549
FAB2890I
Janelia Fluor 646
FAB2890J
PE
FAB2890P
PE/Atto594
FAB2890PEATT594
PE/Cy5.5
FAB2890PECY55
PE/Cy7
FAB2890PECY7
PerCP
FAB2890C
mFluor Violet 450 SE
FAB2890MFV450
mFluor Violet 500 SE
FAB2890MFV500
mFluor Violet 610 SE
FAB2890MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2890-SP
Carrier Free
IC8627G-025
Carrier Free
IC2890V-100UG
Carrier Free
IC2890U-100UG
Carrier Free
IC2890T-100UG
Carrier Free
IC2890S-100UG
Carrier Free
IC2890R-100UG
Carrier Free
IC2890N-100UG
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STAT2 [p Tyr689] Antibody (1021D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing the human STAT2 Y690 site
Modification
p Tyr689
Specificity
Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
STAT2
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 0.05 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using MAB2890.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 [p Tyr689] Antibody (1021D) [Unconjugated]

  • interferon alpha induced transcriptional activator
  • ISGF-3
  • MGC59816
  • P113
  • signal transducer and activator of transcription 2
  • signal transducer and activator of transcription 2, 113kD
  • signal transducer and activator of transcription 2, 113kDa
  • STAT113
  • STAT2

Background

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT2 Antibody (MAB2890)(3)


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAT2 Antibody (MAB2890). (Showing 1 - 1 of 1 FAQs).

  1. Does your phospho-STAT2 antibody (clone number 1021D) cross-react with mouse protein?
    • I have done a blast search between human and mouse STAT2, and it shows that although the tyrosine (Y) residues are conserved in the both species, the adjacent a.a. are different.  Therefore the peptide that was used to raise this antibody should contain the diverse a.a residues. Hence, I anticipate that this Ab will not cross react with mouse sample. 

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STAT2