Home » STAT2 » STAT2 Antibodies » STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]

 (3 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit ...read more
Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1, filled histogram) for 20 minutes was stained with Rabbit Anti-Human Phospho-STAT2 (Y690) ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was ...read more
Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow
Clone
1021D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2890U-100UG
Alexa Fluor 405
IC2890V-100UG
Alexa Fluor 488
IC8627G-025
Alexa Fluor 532
FAB2890X
Alexa Fluor 594
IC2890T-100UG
Alexa Fluor 647
IC2890R-100UG
Alexa Fluor 700
IC2890N-100UG
Alexa Fluor 750
IC2890S-100UG
Allophycocyanin
FAB2890A
Allophycocyanin/Cy7
FAB2890APCCY7
Biotin
FAB2890B
CoraFluor 1
FAB2890CL1
DyLight 350
FAB2890D
DyLight 405
FAB2890E
DyLight 488
FAB2890K
DyLight 550
FAB2890L
DyLight 594
FAB2890M
DyLight 650
FAB2890W
DyLight 680
FAB2890Y
DyLight 755
FAB2890Z
FITC
FAB2890F
HRP
FAB2890H
Janelia Fluor 525
FAB2890JF525
Janelia Fluor 549
FAB2890I
Janelia Fluor 585
FAB2890JF585
Janelia Fluor 635
FAB2890JF635
Janelia Fluor 646
FAB2890J
Janelia Fluor 669
FAB2890JF669
PE
FAB2890P
PE/Atto594
FAB2890PEATT594
PE/Cy5.5
FAB2890PECY55
PE/Cy7
FAB2890PECY7
PerCP
FAB2890C
mFluor Violet 450 SE
FAB2890MFV450
mFluor Violet 500 SE
FAB2890MFV500
mFluor Violet 610 SE
FAB2890MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2890-SP
Carrier Free
IC8627G-025
Carrier Free
IC2890V-100UG
Carrier Free
IC2890U-100UG
Carrier Free
IC2890T-100UG
Carrier Free
IC2890S-100UG
Carrier Free
IC2890R-100UG
Carrier Free
IC2890N-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

STAT2 [p Tyr690] Antibody (1021D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing the human STAT2 Y690 site
Modification
p Tyr690
Specificity
Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
STAT2
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 0.05 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using MAB2890.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]

  • interferon alpha induced transcriptional activator
  • ISGF-3
  • MGC59816
  • P113
  • signal transducer and activator of transcription 2
  • signal transducer and activator of transcription 2, 113kD
  • signal transducer and activator of transcription 2, 113kDa
  • STAT113
  • STAT2

Background

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

STAT1 [p Tyr701]
Antibody [Unconjugate ...
AF2894
Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]
Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
IFN-alpha 1
Antibody
NBP2-75930
Western Blot IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IRF9 Antibody
NBP2-16991
Western Blot IRF9 Antibody
Immunohistochemistry-Paraffin IRF9 Antibody
Western Blot IRF9 Antibody
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Jak1 Antibody
(413104) [Unconjugate
MAB4260
Western Blot Jak1 Antibody (413104) [Unconjugated]
Immunohistochemistry Jak1 Antibody (413104) [Unconjugated]
Intracellular Staining by Flow Cytometry Jak1 Antibody (413104) [Unconjugated]
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
SOAT1 Antibody
NB400-141
SOAT1 Antibody
(7 Publications)
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(22 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(450 Publications)
Species: Hu
Applications: BA
Jak2 Antibody
(53B7) - BSA Free
NBP2-59451
Flow Cytometry Jak2 Antibody (53B7) - BSA Free
Immunocytochemistry/ Immunofluorescence Jak2 Antibody (53B7) - BSA Free
Western Blot Jak2 Antibody (53B7) - BSA Free
(3 Reviews)
(3 Publications)
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
Tyk2 Antibody
(307214) [Unconjugate
MAB3427
Western Blot Tyk2 Antibody (307214) [Unconjugated]
(1 Review)
Species: Hu
Applications: WB
IRF1 Antibody
AF4715
Western Blot IRF1 Antibody
(1 Review)
(2 Publications)
Species: Mu
Applications: WB
STAT5a/b Antibody
[Unconjugated] - Pan ...
AF2168
Western Blot STAT5a/b Antibody [Unconjugated] - Pan Specific
Simple Western STAT5a/b Antibody [Unconjugated] - Pan Specific
Chromatin Immunoprecipitation (ChIP) STAT5a/b Antibody [Unconjugated] - Pan Specific
(28 Publications)
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
STAT5b Antibody
[Unconjugated]
AF1584
Simple Western STAT5b Antibody [Unconjugated]
Knockout Validated STAT5b Antibody [Unconjugated]
Immunocytochemistry STAT5b Antibody [Unconjugated]
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
IFN-beta
[Unconjugated]
8499-IF
Binding Activity IFN-beta [Unconjugated]
(52 Publications)
Species: Hu
Applications: BA
STAT4 [p Tyr693]
Antibody [Unconjugate ...
AF4319
Western Blot STAT4 [p Tyr693] Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(650 Publications)
Species: Mu
Applications: ELISA
CRLF3 Antibody
NBP1-84382
Immunocytochemistry/ Immunofluorescence CRLF3 Antibody
Immunohistochemistry-Paraffin CRLF3 Antibody
Immunohistochemistry-Paraffin CRLF3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Hsp70 interacting
protein HIP Antibody
NBP2-47427
Immunocytochemistry/ Immunofluorescence Hsp70 interacting protein HIP Antibody
Immunohistochemistry-Paraffin Hsp70 interacting protein HIP Antibody
Western Blot Hsp70 interacting protein HIP Antibody
(1 Publication)
Species: Ca, Hu
Applications: ICC/IF, IHC, IHC-P, WB
IFN-alpha/beta R1
Antibody [Unconjugate ...
AF245
Western Blot IFN-alpha/beta R1 Antibody [Unconjugated]
(9 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, WB
STAT6 Antibody
(177C322.1) - BSA Fre
NBP2-25241
Immunocytochemistry/ Immunofluorescence STAT6 Antibody (177C322.1) - BSA Free
Western Blot STAT6 Antibody (177C322.1) - BSA Free
Immunocytochemistry/ Immunofluorescence STAT6 Antibody (177C322.1) - BSA Free
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IP, WB
STAT2 [p Tyr690]
Antibody (1021D) [Unc ...
MAB2890
STAT2 [p Tyr690] Antibody (1021D) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, Flow

Publications for STAT2 Antibody (MAB2890)(3)


Reviews for STAT2 Antibody (MAB2890) (0)

There are no reviews for STAT2 Antibody (MAB2890). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAT2 Antibody (MAB2890). (Showing 1 - 1 of 1 FAQs).

  1. Does your phospho-STAT2 antibody (clone number 1021D) cross-react with mouse protein?
    • I have done a blast search between human and mouse STAT2, and it shows that although the tyrosine (Y) residues are conserved in the both species, the adjacent a.a. are different.  Therefore the peptide that was used to raise this antibody should contain the diverse a.a residues. Hence, I anticipate that this Ab will not cross react with mouse sample. 

Secondary Antibodies

 

Isotype Controls

Additional STAT2 Products

Blogs on STAT2.

Check out the latest blog posts on STAT2.
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STAT2 [p Tyr690] Antibody (1021D) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol STAT2