Is there a recommended antigen retrieval method for this product for use in IHC-P?

Name: p19ARF/CDKN2A Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB200-106
Rating: 3 (1 reviews)

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Accepted Answer

For NB200-106 I would recommend our standard HIER Citrate Buffer pH 6.0.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Mu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB200-106AF350

Alexa Fluor 405 NB200-106AF405

Alexa Fluor 488 NB200-106AF488

Alexa Fluor 532 NB200-106AF532

Alexa Fluor 594 NB200-106AF594

Alexa Fluor 647 NB200-106AF647

Alexa Fluor 700 NB200-106AF700

Alexa Fluor 750 NB200-106AF750

Allophycocyanin NB200-106APC

Biotin NB200-106B

CoraFluor 1 NB200-106CL1

DyLight 350 NB200-106UV

DyLight 405 NB200-106V

DyLight 488 NB200-106G

DyLight 550 NB200-106R

DyLight 594 NB200-106DL594

DyLight 650 NB200-106C

DyLight 680 NB200-106FR

DyLight 755 NB200-106IR

FITC NB200-106F

HRP NB200-106H

Janelia Fluor 525 NB200-106JF525

Janelia Fluor 549 NB200-106JF549

Janelia Fluor 646 NB200-106JF646

Janelia Fluor 669 NB200-106JF669

PE NB200-106PE

PerCP NB200-106PCP

mFluor Violet 450 SE NB200-106MFV450

mFluor Violet 500 SE NB200-106MFV500

mFluor Violet 610 SE NB200-106MFV610

Catalog# & Formulation	Size	Price
BSA Free NB200-106SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-106AF350
Alexa Fluor 405	NB200-106AF405
Alexa Fluor 488	NB200-106AF488
Alexa Fluor 532	NB200-106AF532
Alexa Fluor 594	NB200-106AF594
Alexa Fluor 647	NB200-106AF647
Alexa Fluor 700	NB200-106AF700
Alexa Fluor 750	NB200-106AF750
Allophycocyanin	NB200-106APC
Biotin	NB200-106B
CoraFluor 1	NB200-106CL1
DyLight 350	NB200-106UV
DyLight 405	NB200-106V
DyLight 488	NB200-106G
DyLight 550	NB200-106R
DyLight 594	NB200-106DL594
DyLight 650	NB200-106C
DyLight 680	NB200-106FR
DyLight 755	NB200-106IR
FITC	NB200-106F
HRP	NB200-106H
Janelia Fluor 525	NB200-106JF525
Janelia Fluor 549	NB200-106JF549
Janelia Fluor 646	NB200-106JF646
Janelia Fluor 669	NB200-106JF669
PE	NB200-106PE
PerCP	NB200-106PCP
mFluor Violet 450 SE	NB200-106MFV450
mFluor Violet 500 SE	NB200-106MFV500
mFluor Violet 610 SE	NB200-106MFV610

p19ARF/CDKN2A Antibody - BSA Free

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Publications for p19ARF/CDKN2A Antibody (NB200-106)(48)

Review for p19ARF/CDKN2A Antibody (NB200-106) (1) 31

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Immunogen	Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF/CDKN2A. [Swiss-Prot #Q64364]
Localization	Nuclear
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	Cdkn2a
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Publications using NB200-106	Applications	Species
Zhang Y, Pignolo RJ, Bram RJ. Accelerated Aging in Cyclophilin B-Deficient Mice Downstream of p21-Cip1/Waf1 JBMR Plus 2022-10-01 [PMID: 36248275] (Western Blot)	Western Blot
Ottone OK, Kim CJ, Collins JA, Risbud MV. The cGAS-STING Pathway Affects Vertebral Bone but Does Not Promote Intervertebral Disc Cell Senescence or Degeneration Frontiers in Immunology 2022-06-13 [PMID: 35769461]
Chen H, Zhou J, Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4 Clinical and translational medicine 2022-04-01 [PMID: 35390228] (WB, Mouse)	WB	Mouse
Brelstaff JH, Mason M, Katsinelos T Et al. Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates Science advances 2021-10-22 [PMID: 34669475] (ICC/IF, Mouse)	ICC/IF	Mouse
Risbud M, Novais E, Tran V, et al. Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice Nat Commun 2021-09-04 [PMID: 34480023]
Makino SI, Shirata N, Oliva Trejo JA, et al. Impairment of Proteasome Function in Podocytes Leads to CKD Journal of the American Society of Nephrology : JASN 2021-01-28 [PMID: 33510039]
Rui Y, Chen M, Li Y et al. CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging Stem Cells International 2019-11-11 [PMID: 31827530] (WB, Rat)	WB	Rat
Novais EM, Diekman BO, Shapiro IM, Risbud MV. p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence Matrix Biol. 2019-02-24 [PMID: 30811968] (IHC-P, Mouse)	IHC-P	Mouse
Xie X, Tsai Sy, Tsai Mj. COUP-TFII regulates satellite cell function and muscular dystrophy. J. Clin. Invest. 2016-09-12 [PMID: 27617862]
Chen D, Chen Y, Forrest D, Bremner R. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3 Cell Death Differ 2013-04-05 [PMID: 23558950] (WB, Mouse)	WB	Mouse
Wilson BG, Wang X, Shen X et al. Epigenetic antagonism between polycomb and SWI/SNF complexes during oncogenic transformation. Cancer Cell. 2010-10-19 [PMID: 20951942] (WB, Mouse)	WB	Mouse
Mills JR, Hippo Y, Robert F et al. mTORC1 promotes survival through translational control of Mcl-1. Proc Natl Acad Sci U S A 2008-08-01 [PMID: 18664580]
Wang W-H, Hullinger RL, risani OM. Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283(37):25455-67. 2008-09-12 [PMID: 18606816]
Timmers, C et al. E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop. Mol Cell Biol;27(1):65-78. 2007-01-01 [PMID: 17167174]
Iaquinta PJ, Aslanian A, Lees JA. Regulation of the Arf/p53 tumor surveillance network by E2F. Cold Spring Harb Symp Quant Biol 2005-01-01 [PMID: 16869767] (Mouse)		Mouse
Klochendler-Yeivin, A, Picarsky, E, Yaniv, M. Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex. Mol Cell Biol 26(7):2661-74. 2006-04-01 [PMID: 16537910]
Baker, DJ et al. Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice. J Cell Biol 172(4):529-40. 2006-02-13 [PMID: 16476774]
Castillo JP, Frame FM, Rogoff HA et al. Human cytomegalovirus IE1-72 activates ataxia telangiectasia mutated kinase and a p53/p21-mediated growth arrest response. J Virol 2005-09-01 [PMID: 16103197]
Gapuzan ME, Schmah O, Pollock AD et al. Immortalized fibroblasts from NF-kappaB RelA knockout mice show phenotypic heterogeneity and maintain increased sensitivity to tumor necrosis factor alpha after transformation by v-Ras. Oncogene 2005-09-29 [PMID: 16027734] (Mouse)		Mouse
Samuelson AV, Narita M, Chan HM et al. p400 is required for E1A to promote apoptosis. J Biol Chem 2005-06-10 [PMID: 15741165]
Korgaonkar C, Hagen J, Tompkins V et al. Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function. Mol Cell Biol 2005-02-01 [PMID: 15684379]
Matsumura, Y et al. Resistance of CD1d-/- mice to ultraviolet-induced skin cancer is associated with increased apoptosis. Am J Pathol 165(3):879-87. 2004-09-01 [PMID: 15331412] (IF/IHC, Mouse)	IF/IHC	Mouse
Fehrenbacher N, Gyrd-Hansen M, Poulsen B et al. Sensitization to the lysosomal cell death pathway upon immortalization and transformation. Cancer Res 64(15):5301-10. 2004-08-01 [PMID: 15289336] (WB, Mouse)	WB	Mouse
Baker DJ, Jeganathan KB, Cameron JD et al. BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat Genet 36(7):744-9. 2004-07-01 [PMID: 15208629] (WB, Mouse)	WB	Mouse
Aslanian, A et al. Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics. Genes Dev 18(12):1413-22. 2004-06-15 [PMID: 15175242]
Yi Y, Shepard A, Kittrell F et al. p19ARF determines the balance between normal cell proliferation rate and apoptosis during mammary gland development. Mol Biol Cell15(5):2302-11. 2004-05-01 [PMID: 15105443] (WB, Mouse)	WB	Mouse
Sun, LQ et al. Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG. Genes Dev18(9):1035-46. 2004-05-01 [PMID: 15105378]
Sachs, Z et al. p16(Ink4a) interferes with Abelson virus transformation by enhancing apoptosis. J Virol 78(7):3304-11. 2004-04-01 [PMID: 15016851]
Gong, L et al. Active Akt and functional p53 modulate apoptosis in Abelson virus-transformed pre-B cells. J Virol 78(4):1636-44. 2004-02-01 [PMID: 14747529] (WB, Mouse)	WB	Mouse
Sharpless NE, Ramsey MR, Balasubramanian P et al. The differential impact of p16(INK4a) or p19(ARF) deficiency on cell growth and tumorigenesis. Oncogene 23(2):379-85. 2004-01-15 [PMID: 14724566] (WB, Mouse)	WB	Mouse
Unnikrishnan I, Rosenberg N. Absence of p53 complements defects in Abelson murine leukemia virus signaling. J Virol 77(11):6208-15. 2003-06-01 [PMID: 12743277] (WB, Mouse)	WB	Mouse
Petrenko O, Fingerle-Rowson G, Peng T et al. Macrophage migration inhibitory factor deficiency is associated with altered cell growth and reduced susceptibility to Ras-mediated transformation. J Biol Chem 278(13):11078-85. 2003-03-28 [PMID: 12538581] (WB, Mouse)	WB	Mouse
Nahle Z, Polakoff J, Davuluri RV et al. Direct coupling of the cell cycle and cell death machinery by E2F. Nat Cell Biol 2002-11-01 [PMID: 12389032]
Nambiar PR, Giardina C, Guda K et al. Role of the alternating reading frame (P19)-p53 pathway in an in vivo murine colon tumor model. Cancer Res 62(13):3667-74. 2002-07-01 [PMID: 12097273] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Schmitt CA, Fridman JS, Yang M et al. A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy. Cell. 2002-05-03 [PMID: 12015983] (WB, Mouse)	WB	Mouse
Heron-Milhavet L, LeRoith D. Insulin-like growth factor I induces MDM2-dependent degradation of p53 via the p38 MAPK pathway in response to DNA damage. J Biol Chem 277(18):15600-6. 2002-05-03 [PMID: 11877395] (WB, Mouse)	WB	Mouse
Wood WM, Sarapura VD, Dowding JM et al. Early gene expression changes preceding thyroid hormone-induced involution of a thyrotrope tumor. Endocrinology 2002-02-01 [PMID: 11796486] (Mouse)		Mouse
Rane SG, Cosenza SC, Mettus RV, Reddy EP. Germ line transmission of the Cdk4(R24C) mutation facilitates tumorigenesis and escape from cellular senescence. Mol Cell Biol 2002-01-01 [PMID: 11756559]
Bardeesy N, Morgan J, Sinha M et al. Obligate roles for p16(Ink4a) and p19(Arf)-p53 in the suppression of murine pancreatic neoplasia. Mol Cell Biol. 2002-01-01 [PMID: 11756558] (WB, Mouse)	WB	Mouse
Lee S, Tarn C, Wang WH et al. Hepatitis B virus X protein differentially regulates cell cycle progression in X-transforming versus nontransforming hepatocyte (AML12) cell lines. J Biol Chem 277(10):8730-40. 2002-03-08 [PMID: 11756437] (WB, Mouse)	WB	Mouse
Sharpless NE, Bardeesy N, Lee KH et al. Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis. Nature 413(6851):86-91. 2001-09-06 [PMID: 11544531]
Lin AW, Lowe SW. Oncogenic ras activates the ARF-p53 pathway to suppress epithelial cell transformation. Proc Natl Acad Sci U S A 98(9):5025-30. 2001-04-24 [PMID: 11309506] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
McDoniels-Silvers AL, Herzog CR, Tyson FL et al. Inactivation of both Rb and p53 pathways in mouse lung epithelial cell lines. Exp Lung Res 2001-04-01 [PMID: 11293330] (Mouse)		Mouse
Sage J, Mulligan GJ, Attardi LD et al. Targeted disruption of the three Rb-related genes leads to loss of G(1) control and immortalization. Genes Dev 2000-12-01 [PMID: 11114892] (Mouse)		Mouse
Jenab-Wolcott J, Rodriguez-Correa D, Reitmair AH et al. The absence of Msh2 alters abelson virus pre-B-cell transformation by influencing p53 mutation. Mol Cell Biol 2000-11-01 [PMID: 11046134]
Tournier C, Hess P, Yang DD et al. Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science 288(5467):870-4. 2000-05-05 [PMID: 10797012]
Khan, SH et al. Differential requirement for p19ARF in the p53-dependent arrest induced by DNA damage, microtubule disruption, and ribonucleotide depletion. Proc Natl Acad Sci U S A 97(7):3266-71. 2000-03-28 [PMID: 10716710] (WB, Mouse)	WB	Mouse
Melnikova VO, Bolshakov SV, Walker C et al. Genomic alterations in spontaneous and carcinogen-induced murine melanoma cell lines. Oncogene 23(13):2347-56. 2004-03-25 [PMID: 1474320] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Show All 48 Publications. Collapse Publications.

	Verified Customer 05/07/2018
Application:	IF
Species:	Mouse