Cyclin D1 Antibody (SPM587)

During the cell cycle of most somatic cells, DNA synthesis (S-phase) and mitosis (M-phase) are separated by two gap phases (G1 and G2) of varying duration. Thus, a typical eukaryotic cell sequentially passes through G1, S, G2, and M and back into G1 during a single cycle.12 Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins.13 These proteins are the regulatory subunits of the cyclin dependent kinases (CDKs), which are responsible for the phosphorylation of several cellular targets. Complexes of cyclins and CDKs play a key role in cell cycle control. Within the complexes, the cyclin subunit serves a regulatory role, whereas CDKs have a catalytic protein kinase activity.14 Members of the cyclin family of proteins combine with a CDK subunit to form the active kinase, which initiates G2 to M and G1 to S transition. The latter are controlled by cyclins termed G1 cyclins, which commit the cell to DNA replication. Therefore, the cell cycle can be considered as a cyclin cycle which is controlled by biochemical modifications and formation of complex(es) with CDKs.15 At least five candidate G1-phase cyclins, termed cyclins C, D1 , D2 , D3 , and E have been identified in mammalian cells. Each of these cyclins can associate with one or more of the CDK family members. D-type cyclins are induced during the G1 phase of the mammalian cell cycle in response to a variety of mitogenic growth factors. The three distinct members of the D-type cyclin family are differentially and combinatorially xpressed in various cell lineages. Once induced, the D-type cyclins accumulate in complexes with CDKs, whose kinase activity is thought to be necessary for driving cells into S phase. The major catalytic partners of the D-type cyclins are CDK4 and CDK6, but at least some D-type cyclins also interat with other CDKs, including CDK2 and CDK5. Cyclin D1- and D2-associated CDK4 and/or CDK6 kinase activities have been detected in mid-G1, prior to the activation of any other known CDK, and they culminate in late G1 phase. The cyclin D3-associated CDK4 and/or CDK6 exhibit kinase activities at the G1/S transition. Cyclins D1 , D2 and D3 can be distinguished by their slightly different mobilities on denaturing gels. Under these conditions, the apparent masses are 36, 33-35 and 31-34 kD for cyclins D1 , D2 and D3 , respectively. Because D-type cyclins probably serve as integrators of growth factor-induced signals with the cell cycle clock, aberrant expression of these proteins might play a role in disrupting the normal timing of events governing G1 progression and, in so doing, contribute to oncogenesis. Indeed, a link between tumor formation and inappropriate expression of cyclins has been established.16,17 Overexpression of this protein as a result of chromosomal rearrangement, occurs in parathyroid tumors and centrocytic lymphomas, and amplification of the cyclin D1 gene has been observed in a significant percentage of other cancers, including breast, squamous, and esophageal arcinomas. Immunochemical techniques provide a convenient and sensitive method for detection of these cyclins in human tumor tissues. Such assays facilitate studies directed toward correlating the phenotypic subtypes and aggressiveness of particular human tumors known to exhibit cyclin D1 overexpression and enable studies with other types in which cyclins D2 and D3 are similarly implicated in pathogenesis. The availability of monoclonal antibody reacting specifically with cyclin D1 enables the subcellular detection and localization of cyclin D1 and the measurement of relative differences in cyclin D1 levels as a function of cell cycle phase.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: FLOW, IHC, IHC-P, WB.

Showing Publications 1 - 9 of 9.

Publications using NBP2-32840	Applications	Species
Cai Z, Wang J, Li Y et al. Overexpressed Cyclin D1 and CDK4 proteins are responsible for the resistance to CDK4/6 inhibitor in breast cancer that can be reversed by PI3K/mTOR inhibitors Science China. Life sciences 2022-08-11 [PMID: 35982377]
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (FLOW, Mouse) Details: Citation using the Alexa Fluor 488 version of this antibody.	FLOW	Mouse
Shaalan AK, Teshima THN, Tucker AS, Proctor GB. Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands Cell Death Discovery 2021-01-18 [PMID: 33462217] (IHC)	IHC
Gul HF, Ilhan N, Ilhan N et al. The Combined Effect of Pomegranate Extract and Tangeretin on the DMBA-induced Breast Cancer Model The Journal of nutritional biochemistry 2020-12-13 [PMID: 33326843] (WB, Rat)	WB	Rat
Shi Q, Li Y, Li S et al. LncRNA DILA1 inhibits Cyclin D1 degradation and contributes to tamoxifen resistance in breast cancer Nat Commun 2020-11-02 [PMID: 33139730] (IHC-P, Human)	IHC-P	Human
Xu W, Chen S, Wang X et al. Methylprednisolone potentiates tetrandrine pharmacodynamics against human T lymphoblastoid leukemia MOLT-4 cells via regulation of NF-kappa B activation and cell cycle transition Steroids 2020-08-17 [PMID: 32818521]
Xu W, Wu H, Chen S et al. Cytotoxic effects of vitamins K1, K2 and K3 against human T lymphoblastoid leukemia cells through apoptosis induction and cell cycle arrest Chem Biol Drug Des 2020-04-18 [PMID: 32305047] (Human)		Human
Robinson MH, Maximov V, Lallani S et al. Upregulation of the chromatin remodeler HELLS is mediated by YAP1 in Sonic Hedgehog Medulloblastoma Sci Rep 2019-09-20 [PMID: 31541170] (WB, Mouse)	WB	Mouse
Chelsky ZL, Yue P, Kondratyuk TP et al. A Resveratrol Analogue Promotes ERKMAPK-Dependent Stat3 Serine and Tyrosine Phosphorylation Alterations and Antitumor Effects In Vitro against Human Tumor Cells Mol. Pharmacol. 2015-09-01 [PMID: 26138072] (Human)		Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	01/22/2015	View Summary Application Western Blot Sample Tested human MDAMB-231 whole cell lysate Species Human Lot 595-1XP140415

Array NBP2-32840

• Cyclin D1 Antibodies
• Cyclin D1 ELISA Kits
• Cyclin D1 Lysate
• Cyclin D1 Proteins