| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF |
| Clone | 607121 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E.coli-derived recombinant human RB1 Lys240-Asn406 Accession # P06400 |
| Specificity | Detects human RB1 in Western blots. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RB1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | In Simple Western only 10-15 uL of the recommended dilution is used per data point. |
|
| Publications |
|
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Retinoblastoma 1 protein (RB-1; also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family. Human RB-1 is 928 amino acids in length. The protein contains a Pocket domain (aa 373-771), which is comprised of two other domains, domain A (aa 373-573) and domain B (aa 640-771), and a “spacer” (aa 580-639). The Pocket domain binds to threonine-phosphorylated domain C (aa 771-928), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes. Human RB-1 is 90% aa identical to mouse RB-1. RB-1 is expressed in the retina. The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB-1 lead to the childhood cancer retinoblastoma.
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)