c-Myc Antibody (9E10) - BSA Free

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Drosophila.

We have publications tested in 9 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IP, PLA, Sandwich ELISA, WB.

Showing Publications 1 - 10 of 44. Show All 44 Publications. Collapse Publications.

Publications using NB600-302	Applications	Species
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (WB, Drosophila)	WB	Drosophila
Graziani V, Garcia AR, Alcolado LS et al. Metabolic rewiring in MYC-driven medulloblastoma by BET-bromodomain inhibition Scientific Reports 2023-01-23 [PMID: 36690651] (WB, B/N)	WB, B/N
Jagadeeshaprasad MG, Gautam L, Bewley MC et al. Disulfide bond and crosslinking analyses reveal inter-domain interactions that contribute to the rigidity of placental malaria VAR2CSA structure and formation of CSA binding channel International journal of biological macromolecules 2022-12-05 [PMID: 36470436] (ELISA)	ELISA
Jezek M, Sun W, Negesse MY et al. Set1 regulates telomere function via H3K4 methylation-dependent and -independent pathways and calibrates the abundance of telomere maintenance factors Molecular Biology of the Cell 2023-01-01 [PMID: 36416860] (WB)	WB
Edwards-Hicks J, Su H, Mangolini M et al. MYC sensitises cells to apoptosis by driving energetic demand Nature communications 2022-08-09 [PMID: 35945217] (WB, Human)	WB	Human
Harbauer AB, Hees JT, Wanderoy S et al. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy Neuron 2022-02-19 [PMID: 35216662] (IP, Human)	IP	Human
Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594] Details: Citation using the PE version of this antibody.
Li K, Wang F, Yang ZN et al. TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation Nature communications 2020-12-09 [PMID: 33298911] (WB, PLA, Mouse)	WB, PLA	Mouse
Wang F, Gao Y, Xue S et al. SCARB2 Drives Hepatic Carcinoma Initiation by Supporting Cancer Stem Cell Traits and Enhancing MYC Transcriptional Activity Research Square 2022-02-17 (WB)	WB
Wang F, Gao Y, Xue S et al. SCARB2 drives hepatocellular carcinoma tumor initiating cells via enhanced MYC transcriptional activity Nat Commun 2023-09-22 [PMID: 37739936]
Shen M, Wei Y, Kim H Et al. Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis Nat Cancer 2022-02-05 [PMID: 35121987]
Basu H, Pekkurnaz G, Falk J Et al. FHL2 anchors mitochondria to actin and adapts mitochondrial dynamics to glucose supply The Journal of cell biology 2021-10-04 [PMID: 34342639] (B/N)	B/N
Bewley MC, Gautam L, Jagadeeshaprasad MG et al. Molecular architecture and domain arrangement of the placental malaria protein VAR2CSA suggests a model for carbohydrate binding J Biol Chem 2020-10-29 [PMID: 33122198] (Sandwich ELISA, Bovine)	Sandwich ELISA	Bovine
Zhao L, Han X, Lu J et al. A highly potent PROTAC androgen receptor (AR) degrader ARD-61 effectively inhibits AR-positive breast cancer cell growth in vitro and tumor growth in vivo Neoplasia (New York, N.Y.) 2020-10-01 [PMID: 32928363] (WB)	WB
Schuchner S, Behm C, Mudrak I, Ogris E The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context Sci Signal 2020-01-28 [PMID: 31992583] (WB)	WB
Pedrosa AR, Bodrug N, Gomez-Escudero J et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res. 2019-06-12 [PMID: 31189647] (WB, IP, Mouse)	WB, IP	Mouse
Ida K, Miyamoto T, Higuchi S et al. Effectiveness of a genetic test panel designed for gynecological cancer: an exploratory study Med. Oncol. 2019-05-29 [PMID: 31139954] (IF/IHC, Human)	IF/IHC	Human
Morioka S, Perry JSA, Raymond MH et al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 2018-11-01 [PMID: 30464343] (ICC/IF, Mouse)	ICC/IF	Mouse
Wu J, Fan W, Ma L, Geng X. miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/B-catenin pathway Neuron 2018-10-10 [PMID: 30349197] (WB)	WB
Rodriguez-Muela N, Parkhitko A, Grass T et al. Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes. J. Clin. Invest. 2018-04-19 [PMID: 29672276] (Mouse)		Mouse
Mahadevan S, Sathappan V, Utama B et al. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep 2017-03-20 [PMID: 28317850] (ICC/IF, Mouse)	ICC/IF	Mouse
van der Kant R, Jonker CT, Wijdeven RH et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity J Biol Chem 2015-12-18 [PMID: 26463206] (Human) Details: This reference used the HRP version of NB600-302.		Human
da Costa JP, Carvalhais V, Amado F et al. Anti-tumoral activity of human salivary peptides Peptides. 2015-07-23 [PMID: 26211891] (WB, Human) Details: c-Myc antibody used for Western blot on human cell lines from MG63, HT29, T24 and T-47 cells that were treated with different concentrations of GG peptide (Fig 4).	WB	Human
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun. Nat Commun 2015-04-10 [PMID: 25857523] (WB, Mouse) Details: Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C.	WB	Mouse
Iampietro M, Morissette G, Gravel A, Flamand L. Inhibition of Interleukin-2 Gene Expression by Human Herpesvirus 6B U54 Tegument Protein. J Virol. 2014-08-13 [PMID: 25122797] (WB, Human)	WB	Human
Pekkurnaz G, Trinidad JC, Wang X et al. Glucose Regulates Mitochondrial Motility via Milton Modification by O-GlcNAc Transferase. Cell 2014-07-03 [PMID: 24995978] (ICC/IF) Details: COS7 cells transfected with empty vector or myc-hMilton1 (Figure S3).	ICC/IF
Barnett TC, Liebl D, Seymour LM et al. The Globally Disseminated M1T1 Clone of Group A Streptococcus Evades Autophagy for Intracellular Replication. Cell Host Microbe. 2013-12-11 [PMID: 24331465] (ICC/IF)	ICC/IF
Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013-10-01 [PMID: 23911878]
Hubbi ME, Hu H, Kshitiz et al. Sirtuin-7 Inhibits the Activity of Hypoxia-Inducible Factors. J Biol Chem 2013-07-19 [PMID: 23750001]
Megill A, Lee T, DiBattista AM et al. A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis J Neurosci 2013-05-31 [PMID: 23719799] (ICC/IF)	ICC/IF
Wang H, Shi LZ, Wong CC et al. The Interaction of CtIP and Nbs1 Connects CDK and ATM to Regulate HR-Mediated Double-Strand Break Repair. PLoS Genet 2013-02-01 [PMID: 23468639] (WB)	WB
Lu CS, Truong LN, Aslanian A et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012-10-31 [PMID: 23115235] (IP)	IP
Santini E, Feyder M, Gangarossa G et al. Dopamine- and cAMP-regulated Phosphoprotein of 32-kDa (DARPP-32)-dependent Activation of Extracellular Signal-regulated Kinase (ERK) and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in Experimental Parkinsonism J Biol Chem 2012-08-10 [PMID: 22753408] (IP, Mouse)	IP	Mouse
Wang H, Shao Z, Shi LZ et al. CtIP Protein Dimerization Is Critical for Its Recruitment to Chromosomal DNA Double-stranded Breaks J Biol Chem 2012-06-15 [PMID: 22544744] (WB, IP, Human)	WB, IP	Human
Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW. CutA divalent cation tolerance homolog (E. coli) (CUTA) regulates Beta-cleavage of Beta-amyloid precursor protein (APP) through interacting with Beta-Site APP cleaving protein 1 (BACE1) J Biol Chem 2012-02-17 [PMID: 22351782] (WB, Human)	WB	Human
Kim M-Y, Jung J, Mo J-S et al. The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase. Exp Cell Res. 2011-07-29 [PMID: 21820430]
Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011-06-10 [PMID: 21658608] (IP, WB, Human)	IP, WB	Human
Murri-Plesko MT, Hulikova A, Oosterwijk E et al. Antibody inhibiting enzymatic activity of tumour-associated carbonic anhydrase isoform IX. Eur J Pharmacol. 2011-02-09 [PMID: 21315712] (ELISA, FLOW)	ELISA, FLOW
Silvestri, L et al. Furin-mediated release of soluble hemojuvelin: a new link between hypoxia and iron homeostasis. Blood;111(2):924-31. 2008-01-15 [PMID: 17938254]
Trinkle-Mulcahy L, Chusainow J, Lam YW, Swift S, Lamond A. Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions. Methods Mol Biol;365:133-54. 2007-01-01 [PMID: 17200560] (WB, IP, ICC/IF, Human)	WB, IP, ICC/IF	Human
Bergman, M et al. Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction. Am J Physiol Heart Circ Physiol;292(4):H1847-60. 2007-04-01 [PMID: 17158653] (WB, Mouse)	WB	Mouse
Hilpert K, Hansen G, Wessner H et al. Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng;14(10):803-6. 2001-10-01 [PMID: 11739900]
Bello-Fernandez C, Packham G, ClevelJL. The ornithine decarboxylase gene is a transcriptional target of c-Myc. Proc Natl Acad Sci U S A;90(16):7804-8. 1993-08-15 [PMID: 8356088]
Kari B et al. Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. J Virol;60(2):345-52. 1986-11-01 [PMID: 3021969] (IP, Human)	IP	Human
Show All 44 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	3 reviewed by: Phil Hill	ELISA	Human	05/31/2019	View Summary Application ELISA Sample Tested Serum and Plasma Species Human Lot MAB8594
	5 reviewed by: Verified Customer	WB	Human	11/30/2011	View Summary Application Western Blot Sample Tested Jurkat T-Cells Species Human
	4 reviewed by: Astrid Spannhoff	WB	Human	11/23/2010	View Summary Application Western Blot Sample Tested HeLa cell lysate, Sample Amount: 10 ul Species Human
	5 reviewed by: Verified Customer	WB	Human	08/10/2010	View Summary Application Western Blot Sample Tested 293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug Species Human

Array NB600-302

• c-Myc Antibodies
• c-Myc ELISA Kits
• c-Myc Lysate
• c-Myc Proteins