![Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.](http://images.novusbio.com/fullsize/Jurkat-Whole-Cell-Lysate-Western-Blot-NB800-PC2-img0003.jpg "Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, PAGE |
| Concentration | 1.0 mg/ml |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Publication using NB800-PC2 | Applications | Species |
|---|---|---|
| Smith TB, Dun MD, Smith ND et al. The presence of a truncated base excision repair pathway in human spermatozoa, Mediated by OGG1. J Cell Sci 2013-02-01 [PMID: 23378024] (WB) | WB |
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![SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.](http://images.novusbio.com/fullsize/Jurkat-Whole-Cell-Lysate-SDS-Page-NB800-PC2-img0005.jpg "SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.")