p62/SQSTM1 Antibody - BSA Free

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PKC iota/lambda through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: FLOW, Flow Cytometry, Flow Cytometry Control, WB.

Showing Publications 1 - 8 of 8.

Publications using NBP1-42822	Applications	Species
Lei, Z;Krishnamachary, B;Ritzel, RM;Khan, NZ;Li, Y;Li, H;Brunner, K;Faden, AI;Wu, J; Age-related changes in plasma extracellular vesicles influence neuroinflammation in the brain and neurological outcome after traumatic spinal cord injury Research square 2023-04-17 [PMID: 37131758] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Li Y, Lei Z, Ritzel RM Et al. Impairment of autophagy after spinal cord injury potentiates neuroinflammation and motor function deficit in mice Theranostics 2022-08-01 [PMID: 35910787] (FLOW, Mouse) Details: Citation using the Alexa Fluor 647 version of this antibody.	FLOW	Mouse
Giretova M, Medvecky L, Petrovova E et al. Polycystin-2 Is Required for Starvation- and Rapamycin-Induced Atrophy in Myotubes Front Endocrinol (Lausanne) 2019-05-08 [PMID: 31133985] (WB, Mouse)	WB	Mouse
Lin YJ, Liang WM, Chen CJ et al. Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells Phytomedicine 2019-03-13 [PMID: 30901663] (WB, Human)	WB	Human
Pena-Oyarzun D, Troncoso R, Kretschmar C et al. Hyperosmotic stress stimulates autophagy via polycystin-2 Oncotarget 2017-08-22 [PMID: 28915568] (WB, Human)	WB	Human
Zhang S, Gui Xh, Huang Lp et al. Neuroprotective Effects of B-Asarone Against 6-Hydroxy Dopamine-Induced Parkinsonism via JnK/Bcl-2/Beclin-1 Pathway. Mol. neurobiol. 2014-11-18 [PMID: 25404088] (WB, Rat)	WB	Rat
Ritzel R, Li Y, Lei Z Et al. Age-related Dysregulation of Autophagy Contributes to Microglial Dysfunction and Chronic Neurobehavioral Deficits After Traumatic Brain Injury Research Square 2021-11-11 (Flow Cytometry Control, Mouse)	Flow Cytometry Control	Mouse
Ritzel RM, Li Y, Lei Z et al. Functional and transcriptional profiling of microglial activation during the chronic phase of TBI identifies an age-related driver of poor outcome in old mice GeroScience 2022-04-22 [PMID: 35451674] (FLOW, Mouse)	FLOW	Mouse

Array NBP1-42822

• p62/SQSTM1 Antibodies
• p62/SQSTM1 ELISA Kit
• p62/SQSTM1 Lysate
• p62/SQSTM1 Proteins