p62/SQSTM1 Antibody - BSA Free

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PKC iota/lambda through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.

Showing Publications 1 - 10 of 10.

Publications using NBP1-42821	Applications	Species
Amanda JM, Taylor ED, Artem NP et al. Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D Autophagy. 2024-04-09 [PMID: 38591519]
Yazdankhah M, Ghosh S, Liu H et al. Mitophagy in Astrocytes Is Required for the Health of Optic Nerve Cells 2023-10-20 [PMID: 37887340]
Li J, Ding R, Gao H et al. New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway RSC Adv 2022-05-11 [PMID: 35540656]
Yazdankhah M, Ghosh S, Shang P, et al. BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes Autophagy 2021-01-19 [PMID: 33404293] (WB, Rat)	WB	Rat
Geng X, Wang F, Tian D et al. Cardiac glycosides inhibit cancer through Na/K-ATPase-dependent cell death induction Biochem. Pharmacol. 2020-09-22 [PMID: 32976831] (WB, Human)	WB	Human
Wang F, Mayca Pozo F, Tian D et al. Shikonin Inhibits Cancer Through P21 Upregulation and Apoptosis Induction Front Pharmacol 2020-06-09 [PMID: 32581812] (WB, Human)	WB	Human
Liu X, Feng B, Vats A et al. Pharmacological clearance of misfolded rhodopsin for the treatment of RHO-associated retinitis pigmentosa The FASEB Journal 2020-08-01 [PMID: 32536017]
Yazdankhah M, Shang P, Ghosh S et al. Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease Autophagy 2019-09-01 [PMID: 31462148] (WB, Rat)	WB	Rat
Shivapathasundram G, Wickremesekera A, Brasch H et al. SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis Autophagy 2019-05-01 [PMID: 30696333] (WB, Mouse)	WB	Mouse
Eason RJ, Bell KS, Marshall FA et al. The helminth product, ES-62 modulates dendritic cell responses by inducing the selective autophagolysosomal degradation of TLR-transducers, as exemplified by PKCdelta. Sci Rep. 2016-11-21 [PMID: 27869138] (WB, Mouse)	WB	Mouse

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Armen Petrosyan	IF	Mouse	11/19/2024	View Summary Application Immunofluorescence Sample Tested Primary mouse hepatocytes Species Mouse Lot B-2
	5 reviewed by: Armen Petrosyan	WB	Rat	06/27/2023	View Summary Application Western Blot Sample Tested Rat hepatocytes Species Rat Lot B-1
	5 reviewed by: Armen Petrosyan	WB	Mouse	06/27/2023	View Summary Application Western Blot Sample Tested Primary mouse hepatocytes Species Mouse Lot B-1
	5 reviewed by: Armen Petrosyan	Lysate of HepG2 cells	Human	06/27/2023	View Summary Application Other Sample Tested HepG2 Species Human Lot B-1
Enlarge	5 reviewed by: Bryan Tinsley	ICC	Human	06/12/2014	View Summary Application Immunocytochemistry Species Human

Array NBP1-42821

• p62/SQSTM1 Antibodies
• p62/SQSTM1 ELISA Kit
• p62/SQSTM1 Lysate
• p62/SQSTM1 Proteins