![Immunocytochemistry/Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. Nephrin levels were analyzed by immunofluorescence assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-36911-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77303-img0017.jpg "Immunocytochemistry/Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. Nephrin levels were analyzed by immunofluorescence assay. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-36911-1) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. Amino Acid Sequence: EPDSLPFELRGHLV |
| Specificity | Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). NBP1-77303 can detect isoforms of human, mouse and rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NPHS1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0015.jpg "Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.")
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated with PDTC or AS-IV for 48hours. The levels of TGF-, nephrin, E-cadherin, N-cadherin and -SMA were quantified with Western blotting. Data is presented as meanSD. n=3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P <0.05. Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT-NF-kB p65 axis. Sci Rep (2019)](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0016.jpg "Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated with PDTC or AS-IV for 48hours. The levels of TGF-, nephrin, E-cadherin, N-cadherin and -SMA were quantified with Western blotting. Data is presented as meanSD. n=3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P <0.05. Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT-NF-kB p65 axis. Sci Rep (2019)")
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-NBP1-77303-img0004.jpg "Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.")
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Western-Blot-NBP1-77303-img0001.jpg "Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. alpha -SMA & nephrin levels in vivo were analyzed by immunofluorescence assay & the levels of TGF-beta , N-cadherin & E-cadherin were quantified using Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal control cohort, or AS-IV treated cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202415291992.jpg "Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. alpha -SMA & nephrin levels in vivo were analyzed by immunofluorescence assay & the levels of TGF-beta , N-cadherin & E-cadherin were quantified using Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal control cohort, or AS-IV treated cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - AS-IV effects on hyperglycaemia-triggered podocyte EMT. (A) AS-IV chemical structure. (B–E) Podocytes were pretreated with high/normal glucose for 1 hour, & then incubated with or without AS-IV (25, 50 & 100 μM) for 48 hours. (B) mRNA expression levels of TGF-beta were detected using real-time PCR. (C) TGF-beta protein levels were quantified using Western blotting. (D) mRNA expression levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using real-time PCR. (E) Protein levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using Western blotting. Note: E-Ca, E-cadherin; N-Ca, N-cadherin. The molecular weight of the proteins: TGF-beta , 44 kDa; nephrin, 100 kDa; E-cadherin, 110 kDa; N-cadherin, 100 kDa; alpha -SMA, 42 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort or AS-IV cohort, P < 0.05; #compared with high glucose cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202416165647.jpg "Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - AS-IV effects on hyperglycaemia-triggered podocyte EMT. (A) AS-IV chemical structure. (B–E) Podocytes were pretreated with high/normal glucose for 1 hour, & then incubated with or without AS-IV (25, 50 & 100 μM) for 48 hours. (B) mRNA expression levels of TGF-beta were detected using real-time PCR. (C) TGF-beta protein levels were quantified using Western blotting. (D) mRNA expression levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using real-time PCR. (E) Protein levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using Western blotting. Note: E-Ca, E-cadherin; N-Ca, N-cadherin. The molecular weight of the proteins: TGF-beta , 44 kDa; nephrin, 100 kDa; E-cadherin, 110 kDa; N-cadherin, 100 kDa; alpha -SMA, 42 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort or AS-IV cohort, P < 0.05; #compared with high glucose cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - In vitro AS-IV effects on SIRT1 & NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT. (A–F) Podocytes were exposed to either a SIRT1 inhibitor EX527 or SIRT1 activator SRT1720 after a 1 hour exposure to hyperglycaemic conditions, & were subsequently incubated with or without AS-IV (100 μM) for 48 hours. (A,E) mRNA expression levels of SIRT1and p65 were quantified using real-time PCR. (B) The deacetylase activity of SIRT1 was detected with a SIRT1 activity assay. (C,D,F) The protein levels of SIRT1 & AC-p65 were quantified using Western blotting. The molecular weight of the proteins: SIRT1, 110 kDa; AC-p65, 65 kDa; p65, 65 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or AS-IV cohort, or high glucose plus AS-IV cohort, or high glucose plus SRT1720 cohort, P < 0.05; #compared with high glucose plus AS-IV cohort, P < 0.05. (G) Podocytes were pretreated with high glucose for 1 hour, & then incubated with PDTC or AS-IV for 48 hours. The levels of TGF-beta , nephrin, E-cadherin, N-cadherin & alpha -SMA were quantified with Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-310202416163744.jpg "Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - In vitro AS-IV effects on SIRT1 & NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT. (A–F) Podocytes were exposed to either a SIRT1 inhibitor EX527 or SIRT1 activator SRT1720 after a 1 hour exposure to hyperglycaemic conditions, & were subsequently incubated with or without AS-IV (100 μM) for 48 hours. (A,E) mRNA expression levels of SIRT1and p65 were quantified using real-time PCR. (B) The deacetylase activity of SIRT1 was detected with a SIRT1 activity assay. (C,D,F) The protein levels of SIRT1 & AC-p65 were quantified using Western blotting. The molecular weight of the proteins: SIRT1, 110 kDa; AC-p65, 65 kDa; p65, 65 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or AS-IV cohort, or high glucose plus AS-IV cohort, or high glucose plus SRT1720 cohort, P < 0.05; #compared with high glucose plus AS-IV cohort, P < 0.05. (G) Podocytes were pretreated with high glucose for 1 hour, & then incubated with PDTC or AS-IV for 48 hours. The levels of TGF-beta , nephrin, E-cadherin, N-cadherin & alpha -SMA were quantified with Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Nephrin antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-2711202420324873.jpg "Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Nephrin antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney cells labeling Nephrin with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-271120242019531.jpg "Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney cells labeling Nephrin with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Rat Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Nephrin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77303_rabbit-polyclonal-nephrin-antibody-271120242092077.gif "Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Rat Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Nephrin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")