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Podocalyxin Like Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated ...read more
Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Defects in TRAF3IP1 mutants are mediated by MAP4.(a) Lateral views of WT zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in WT embryos injected with control or map4 morpholino. (b) ...read more
TRAF3IP1 mutations lead to epithelialization and polarity defects.(a) mIMCD3 cells grown until confluence on filters were subjected to Ca2+-free medium to disrupt the tight junctions. Six hours after Ca2+ addition, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Podocalyxin Like Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1556 in the following applications:

Publications
Read Publications using
AF1556 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody [Unconjugated]

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podocalyxin Like Antibody (AF1556)(101)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenograft.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
ICC
(5)
IHC
(17)
IHC-Fr
(9)
IHC-P
(5)
Immunohistochemistry
(4)
Western Blot
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(38)
Rat
(2)
Transgenic Mouse
(3)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 101. Show All 101 Publications.
Publications using AF1556 Applications Species
Cybulsky, AV;Papillon, J;Guillemette, J;Navarro-Betancourt, JR;Elimam, H;Fantus, IG; Genetic deletion of calcium-independent phospholipase A2? protects mice from diabetic nephropathy PloS one 2024-10-31 [PMID: 39480824] (Western Blot, Mouse) Western Blot Mouse
Janes, PW;Parslow, AC;Cao, D;Rigopoulos, A;Lee, FT;Gong, SJ;Cartwright, GA;Burvenich, IJG;Eriksson, U;Johns, TG;Scott, FE;Scott, AM; An Anti-VEGF-B Antibody Reduces Abnormal Tumor Vasculature and Enhances the Effects of Chemotherapy Cancers 2024-05-16 [PMID: 38791979] (Immunohistochemistry, Xenograft) Immunohistochemistry Xenograft
Niasse, A;Louis, K;Lenoir, O;Schwarz, C;Xu, X;Couturier, A;Dobosziewicz, H;Corchia, A;Placier, S;Vandermeersch, S;Hennighausen, L;Frère, P;Galichon, P;Surin, B;Ouchelouche, S;Louedec, L;Migeon, T;Verpont, MC;Yousfi, N;Buob, D;Xu-Dubois, YC;François, H;Rondeau, E;Mesnard, L;Hadchouel, J;Luque, Y; Protective Role of the Podocyte IL-15 / STAT5 Pathway in Focal Segmental Glomerulosclerosis Kidney international reports 2024-04-01 [PMID: 38765560] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Moritz Schanz, Martin Kimmel, Mark Dominik Alscher, Kerstin Amann, Christoph Daniel TIMP-2 and IGFBP7 in human kidney biopsies in renal disease Clinical Kidney Journal 2023-08-31 [PMID: 37664566]
Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA; Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction Journal of the American Heart Association 2023-06-22 [PMID: 37345826] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Chen, Y;Gong, C;Chauhan, S;De La Cruz, ED;Datta, MS;Tomer, R; Scalable projected Light Sheet Microscopy for high-resolution imaging of large samples bioRxiv : the preprint server for biology 2023-06-05 [PMID: 37333196] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Chen, X;Wolfe, DA;Bindu, DS;Zhang, M;Taskin, N;Goertsen, D;Shay, TF;Sullivan, EE;Huang, SF;Ravindra Kumar, S;Arokiaraj, CM;Plattner, VM;Campos, LJ;Mich, JK;Monet, D;Ngo, V;Ding, X;Omstead, V;Weed, N;Bishaw, Y;Gore, BB;Lein, ES;Akrami, A;Miller, C;Levi, BP;Keller, A;Ting, JT;Fox, AS;Eroglu, C;Gradinaru, V; Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates Nature communications 2023-06-08 [PMID: 37291094] (IHC, Mouse) IHC Mouse
Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong et al. Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse Frontiers in Cell and Developmental Biology 2023-05-05 [PMID: 37215091]
Satu Paavonsalo, Yelin Subashi, Madeleine H. Lackman, Sinem Karaman Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine STAR Protocols 2023-06-01 [PMID: 37182207]
Xinhong Chen, Damien A. Wolfe, Dhanesh Sivadasan Sivadasan Bindu, Mengying Zhang, Naz Taskin, David Goertsen et al. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates bioRxiv 2023-01-18 [PMID: 36711773]
Show All 101 Publications.

Reviews for Podocalyxin Like Antibody (AF1556) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using AF1556:
Filter by Applications
IHC-P
(1)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Rat 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24100472
SpeciesRat
  5
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23338608
SpeciesMouse
  4
reviewed by:
Verified Customer
ICC Human 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24306541
SpeciesHuman

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FAQs for Podocalyxin Like Antibody (AF1556) (0)

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Recent Reviews

4.7
5
2
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1
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2
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1
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Verified Customer
01/05/2015
Application: IHC-P
Species: Rat

 
Verified Customer
01/05/2015
Application: IF
Species: Mouse

 
Verified Customer
01/05/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PODXL
Uniprot