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Goat IgG Isotype Control [Unconjugated]

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SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more
Decreased macrophage recruitment correlates with decreased angiogenesis.A) Quantification of BrdU incorporation demonstrates that decreased macrophage infiltration does not significantly correlate with a change in ...read more
iFGFR1 activation in vivo promotes recruitment of CX3CR1-positive macrophages.MMTV-iFGFR1 transgenic mice were treated with B/B in order to analyze the population of macrophages that are recruited to the mammary ...read more
iFGFR1 activation in vivo promotes recruitment of CX3CR1-positive macrophages.MMTV-iFGFR1 transgenic mice were treated with B/B in order to analyze the population of macrophages that are recruited to the mammary ...read more
Blocking the tissue inhibitor of metalloproteinase type 1 (TIMP-1) in MSC-CM reduced its pro-oligodendroglial activity on cultured aNSCs. Representative immunocytochemical staining of 2′,3′-cyclic-nucleotide ...read more
Localisation of Sox17 staining in proliferative and secretory phase endometrial tissue and regulation by hormones. Within the proliferative (A) and secretory (B) endometrium, Sox17 localized to the glandular (open ...read more
Knockdown of SOX17 results in reduced expression in endometrial luminal epithelial cells. Transfection of ECC-1 cells with a CRISPR/Cas9 SOX17 knock down plasmid resulted in decreased SOX17 expression (KD1-5) when ...read more
Osteoclast resorption of bony interspinous ligaments releases active TGF-beta to drive the progression of ossification in AS patients. e Immunostaining and f quantitative analysis of CD68-positive cells (brown) in the ...read more
Progression of endochondral heterotopic ossification in AS patients. c Immunostaining and d quantitative analysis of collagen II-positive cells (brown) in the normal and chondrogenic interspinous ligaments (sagittal ...read more
Chondrocyte differentiation and cartilage formation in interspinous ligaments of AS patients before calcification. a Hematoxylin and eosin (H&E) staining and b Safranin O–Fast Green (SOFG) staining of normal and ...read more
Progression of endochondral heterotopic ossification in AS patients. i Immunostaining and j quantitative analysis of pSmad2/3-positive cells (brown) in the normal ligaments and endochondral-ossified interspinous ...read more
Chondrocyte differentiation and cartilage formation in interspinous ligaments of AS patients before calcification. a Hematoxylin and eosin (H&E) staining and b Safranin O–Fast Green (SOFG) staining of normal and ...read more
Elevated TGF-beta levels in the early inflammatory stage of AS. a Hematoxylin and eosin (H&E) staining and b Safranin O–Fast Green (SOFG) staining of normal and inflamed interspinous ligaments. In the AS group, the ...read more
Osteoclast resorption of bony interspinous ligaments releases active TGF-beta to drive the progression of ossification in AS patients. g Immunostaining and h quantitative analysis of pSmad2/3-positive cells in the ...read more
Progression of endochondral heterotopic ossification in AS patients.g Immunostaining and h quantitative analysis of the number of CD68-positive osteoclast (brown) surface (OCS) per bone surface (BS). The bottom panels ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Dilutions
  • Control
Reviewed Applications
Read 2 Reviews rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (AB-108-C)(626)

We have publications tested in 18 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Bovine, Canine, Chicken, Ferret, Guinea Pig, Hamster, Porcine, Primate, Primate - C. aethiops, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse, Transgenic Rat, Xenograft.

We have publications tested in 37 applications: Binding Assay, Binding Control, Bioassay, Blocking, Cell Culture, ChIP, ChIP (chromatin immunoprecipit, ChIP Control, Co-Immunoprecipitation, Control, ELISA Control, Flow Cytometry, Flow Cytometry Control, Functional Assay, Functional Assay - Control, Functional Assay Control, ICC, ICC Control, IHC, IHC Control, IHC-F, IHC-Fr, IHC-P, Immunohistochemistry, Immunoprecipitation, Immunoprecipitation Control, In Vivo, In Vivo Control, In vivo assay, Isotype Control, Neutralization, Neutralization Control, RNA Immunoprecipitation Contro, RNA immunoprecipitation, Tissue Culture, Western Blot, Western Blot Control.


Filter By Application
Binding Assay
(1)
Binding Control
(1)
Bioassay
(13)
Blocking
(1)
Cell Culture
(6)
ChIP
(6)
ChIP (chromatin immunoprecipit
(1)
ChIP Control
(1)
Co-Immunoprecipitation
(2)
Control
(118)
ELISA Control
(1)
Flow Cytometry
(17)
Flow Cytometry Control
(4)
Functional Assay
(9)
Functional Assay - Control
(1)
Functional Assay Control
(1)
ICC
(8)
ICC Control
(2)
IHC
(17)
IHC Control
(8)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(5)
Immunohistochemistry
(1)
Immunoprecipitation
(9)
Immunoprecipitation Control
(3)
In Vivo
(15)
In Vivo Control
(4)
In vivo assay
(3)
Isotype Control
(34)
Neutralization
(53)
Neutralization Control
(49)
RNA Immunoprecipitation Contro
(1)
RNA immunoprecipitation
(1)
Tissue Culture
(1)
Western Blot
(5)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(177)
Mouse
(185)
Rat
(25)
Bacteria - Streptococcus pyogenes
(1)
Bovine
(1)
Canine
(1)
Chicken
(2)
Ferret
(1)
Guinea Pig
(1)
Hamster
(2)
Porcine
(4)
Primate
(1)
Primate - C. aethiops
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rabbit
(1)
Transgenic Mouse
(4)
Transgenic Rat
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 626. Show All 626 Publications.
Publications using AB-108-C Applications Species
Song, M;Deng, M;Peng, Z;Dai, F;Wang, Y;Shu, W;Zhou, X;Zhang, J;Hou, Y;Yu, B; Granulocyte colony-stimulating factor mediates bone loss via the activation of IL-1?/JNK signaling pathway in murine Staphylococcus aureus-induced osteomyelitis International immunopharmacology 2024-08-19 [PMID: 39163688] (In vivo assay, Mouse) In vivo assay Mouse
Jotatsu, Y;Sung, SY;Wu, MH;Takeda, S;Hirata, Y;Maeda, K;Fang, SB;Chen, KC;Shigemura, K; An Antibody of the Secreted Isoform of Disintegrin and Metalloprotease 9 (sADAM9) Inhibits Epithelial-Mesenchymal Transition and Migration of Prostate Cancer Cell Lines International journal of molecular sciences 2024-06-17 [PMID: 38928352] (Neutralization Control, Human, Mouse) Neutralization Control Human, Mouse
Moon, S;Han, S;Jang, IH;Ryu, J;Rha, MS;Cho, HJ;Yoon, SS;Nam, KT;Kim, CH;Park, MS;Seong, JK;Lee, WJ;Yoon, JH;Chung, YW;Ryu, JH; Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection Nature communications 2024-04-30 [PMID: 38693120] (Neutralization Control, Mouse) Neutralization Control Mouse
Saddouk, FZ;Kuzemczak, AP;Saito, J;Greif, DM; Endothelial HIF?-PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension JCI insight 2024-04-23 [PMID: 38652543] (Neutralization Control, Human) Neutralization Control Human
Harary Søndergaard, R;Drozd Højgaard, L;Haack-Sørensen, M;Hoeeg, C;Mønsted Johansen, E;Follin, B;Kastrup, J;Ekblond, A;Juhl, M; Investigating the paracrine and juxtacrine abilities of adipose-derived stromal cells in angiogenesis triple cell co-cultures Stem cell research 2024-04-09 [PMID: 38608355] (Neutralization Control, Human) Neutralization Control Human
Le Tran, N;Wang, Y;Bilandzic, M;Stephens, A;Nie, G; Podocalyxin promotes the formation of compact and chemoresistant cancer spheroids in high grade serous carcinoma Scientific reports 2024-03-30 [PMID: 38553472] (Immunohistochemistry, Human) Immunohistochemistry Human
Tufan, T;Comertpay, G;Villani, A;Nelson, GM;Terekhova, M;Kelley, S;Zakharov, P;Ellison, RM;Shpynov, O;Raymond, M;Sun, J;Chen, Y;Bockelmann, E;Stremska, M;Peterson, LW;Boeckaerts, L;Goldman, SR;Etchegaray, JI;Artyomov, MN;Peri, F;Ravichandran, KS; Rapid unleashing of macrophage efferocytic capacity via transcriptional pause release Nature 2024-03-13 [PMID: 38480883] (Functional Assay, Human) Functional Assay Human
Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB; Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity Cell reports 2024-03-07 [PMID: 38457336] (Functional Assay, Human) Functional Assay Human
Silveira Prudente, A;Hoon Lee, S;Roh, J;Luckemeyer, DD;Cohen, CF;Pertin, M;Park, CK;Suter, MR;Decosterd, I;Zhang, JM;Ji, RR;Berta, T; Microglial STING activation alleviates nerve injury-induced neuropathic pain in male but not female mice Brain, behavior, and immunity 2024-01-06 [PMID: 38190983] (In vivo assay, Mouse) In vivo assay Mouse
Justynski, O;Bridges, K;Krause, W;Forni, MF;Phan, QM;Sandoval-Schaefer, T;Carter, K;King, DE;Hsia, HC;Gazes, MI;Vyce, SD;Driskell, RR;Miller-Jensen, K;Horsley, V; Apoptosis recognition receptors regulate skin tissue repair in mice eLife 2023-12-21 [PMID: 38127424] (In Vivo, Mouse) In Vivo Mouse
Show All 626 Publications.

Reviews for IgG Isotype Control (AB-108-C) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using AB-108-C:
Filter by Applications
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Filter by Species
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
05/07/2019
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Summary

Lotes4118071
 IgG AB-108-C
Enlarge
5
reviewed by:
Verified Customer
04/07/2017
View

Summary

LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Verified Customer
05/07/2019
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Verified Customer
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Bioinformatics

Gene Symbol IGHG1