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CD34 Antibody [Unconjugated]

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CD34 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Rat CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4117) overnight at 4 °C. Tissue was stained with the ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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CD34 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat CD34
Glu37-Ile293
Accession # NP_001100672
Specificity
Detects rat CD34 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant porcine CD34 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD34
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
AF4117 in the following applications:

Publications
Read Publications using
AF4117 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD34 Antibody [Unconjugated]

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD34 Antibody (AF4117)(37)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC
(4)
IHC
(9)
IHC-Fr
(2)
IHC-P
(3)
All Applications
Filter By Species
Mouse
(1)
Rat
(17)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using AF4117 Applications Species
H Wang, H Segersvärd, J Siren, S Perttunen, K Immonen, R Kosonen, YC Chen, J Tolva, M Laivuori, MI Mäyränpää, PT Kovanen, J Sinisalo, M Laine, I Tikkanen, P Lakkisto Tankyrase Inhibition Attenuates Cardiac Dilatation and Dysfunction in Ischemic Heart Failure International Journal of Molecular Sciences, 2022-09-02;23(17):. 2022-09-02 [PMID: 36077457] (IHC, Rat) IHC Rat
L Carreres, M Mercey-Res, K Kurma, J Ghelfi, C Fournier, O Manches, F Chuffart, S Rousseaux, M Minoves, T Decaens, H Lerat, Z Macek Jilk Chronic Intermittent Hypoxia Increases Cell Proliferation in Hepatocellular Carcinoma Cells, 2022-06-28;11(13):. 2022-06-28 [PMID: 35805134] (IHC, Rat) IHC Rat
JongWoong Park, Ji Hun Shin, Myoung-Ryul Kim, JungIl Gwon, Hae Won Hwang, Yeong-Rim Jung et al. Delivery of nitric oxide-releasing silica nanoparticles for in vivo revascularization and functional recovery after acute peripheral nerve crush injury Neural Regeneration Research 2/10/2022 [PMID: 35142695]
K Kurma, O Manches, F Chuffart, N Sturm, K Gharzeddin, J Zhang, M Mercey-Res, S Rousseaux, A Millet, H Lerat, PN Marche, Z Macek Jilk, T Decaens DEN-Induced Rat Model Reproduces Key Features of Human Hepatocellular Carcinoma Cancers, 2021-10-04;13(19):. 2021-10-04 [PMID: 34638465] (IHC, Rat) IHC Rat
Fang Huang, Yu-Fang Hsieh, Xuefeng Qiu, Shyam Patel, Song Li Engineering the Composition of Microfibers to Enhance the Remodeling of a Cell-Free Vascular Graft Nanomaterials (Basel) 6/20/2021 [PMID: 34202961]
T Nakamura, U Yokoyama, T Kanaya, T Ueno, T Yoda, A Ishibe, Y Hidaka, M Umemura, T Takayama, M Kaneko, S Miyagawa, Y Sawa, I Endo, Y Ishikawa Multilayered Human Skeletal Muscle Myoblast Sheets Promote the Healing Process After Colonic Anastomosis in Rats Cell Transplantation, 2021-01-01;30(0):9636897211009. 2021-01-01 [PMID: 33880968] (IHC, Rat) IHC Rat
Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 2020-10-02;20(0):476. 2020-10-02 [PMID: 33024414] (IHC, Mouse) IHC Mouse
S Ullsten, D Espes, M Quach, M Fex, M Sandberg, PO Carlsson Highly blood perfused, highly metabolically active pancreatic islets may be more susceptible for immune attack Physiol Rep, 2020-07-01;8(13):e14444. 2020-07-01 [PMID: 32618430] (IHC, Rat) IHC Rat
Chao Liu, Jiao Sun Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen Experimental and Therapeutic Medicine 4/22/2020 [PMID: 32509014]
BE Fornasari, M El Soury, G Nato, A Fucini, G Carta, G Ronchi, A Crosio, I Perroteau, S Geuna, S Raimondo, G Gambarotta Fibroblasts Colonizing Nerve Conduits Express High Levels of Soluble Neuregulin1, a Factor Promoting Schwann Cell Dedifferentiation Cells, 2020-06-01;9(6):. 2020-06-01 [PMID: 32492853] (IHC, Rat) IHC Rat
Show All 37 Publications.

Reviews for CD34 Antibody (AF4117) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Rat.

Reviews using AF4117:
Filter by Applications
IHC-P
(2)
ICC
(1)
All Applications
Filter by Species
Rat
(3)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Rat 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 25072110
SpeciesRat
  4
reviewed by:
Verified Customer
IHC-P Rat 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22361334
SpeciesRat
  4
reviewed by:
Verified Customer
ICC Rat 01/08/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24670794
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD34 Antibody (AF4117). (Showing 1 - 1 of 1 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

Additional CD34 Products

Blogs on CD34.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD ...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial c...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

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Recent Reviews

4
5
0
4
3
3
0
2
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1
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Verified Customer
01/08/2015
Application: IHC-P
Species: Rat

 
Verified Customer
01/08/2015
Application: IHC-P
Species: Rat

 
Verified Customer
01/08/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol CD34
Uniprot