| Reactivity | MuSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse CD45 Gln24-Lys425 Accession # NP_035340 |
| Specificity | Detects mouse CD45 in direct ELISAs and Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PTPRC |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.
![Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Simple_Western_21377.jpg)
![Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Western_Blot_21459.jpg)
![Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Immunohistochemistry_17361.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
IP | Other | 01/05/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
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Read full blog post. |
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How To Identify B Cell Subsets Using Flow Cytometry By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified... Read full blog post. |
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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t... Read full blog post. |
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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells. Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol... Read full blog post. |
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CD45 - Much more than just a housekeeping protein CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le... Read full blog post. |
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CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,... Read full blog post. |
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Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi... Read full blog post. |
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Antibodies In The Differential Diagnosis Of Undifferentiated Tumors Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in... Read full blog post. |
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