Reactivity | Hu, Mu, Rt, Po, Ca, RbSpecies Glossary |
Applications | WB, Simple Western, Flow, ICC/IF, IHC, Dual ISH-IHC |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | A synthetic peptide made to an C-terminal region of the human CD4 Antibody protein (within residues 400-458). [Swiss-Prot P01730] |
Localization | Membrane |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | CD4 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Application Notes | This CD4 antibody is useful for ICC, Western Blot, and IHC-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Sprague-Dawley rat | 01/04/2019 |
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 06/05/2018 |
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reviewed by:
Manuel Flores |
IHC-Fr | Mouse | 03/16/2017 |
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reviewed by:
Verified Customer |
IHC-P | Mouse | 10/06/2015 |
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reviewed by:
Merissa Olmer |
IHC-P | 10/04/2014 |
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reviewed by:
Verified Customer |
WB | Other | 09/19/2014 |
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reviewed by:
Verified Customer |
WB | 08/28/2014 |
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reviewed by:
Rahul Mittal |
Flow | Human | 04/24/2010 |
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reviewed by:
Rahul Mittal |
IHC-P | Mouse | 04/22/2010 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CD4 Antibody (NBP1-19371)Find related products by research area.
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Verified Customer 01/04/2019 |
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Application: | IHC-P | |
Species: | Sprague-Dawley rat |
Verified Customer 06/05/2018 |
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Application: | IHC-Fr | |
Species: | Mouse |
Manuel Flores 03/16/2017 |
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Application: | IHC-Fr | |
Species: | Mouse |