I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB17781
Price: 469 USD

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Accepted Answer

I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, IP, B/N, CyTOF-ready, ICC/IF, KO
Clone	P5D2
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB17781U-100UG

Alexa Fluor 405 FAB17781V-100UG

Alexa Fluor 488 FAB17781G-100UG

Alexa Fluor 532 FAB17781X

Alexa Fluor 594 FAB17781T-100UG

Alexa Fluor 647 FAB17781R-100UG

Alexa Fluor 700 FAB17781N-100UG

Alexa Fluor 750 FAB17781S-100UG

Allophycocyanin FAB17781A

Allophycocyanin/Cy7 FAB17781APCCY7

Biotin FAB17781B

CoraFluor 1 FAB17781CL1

DyLight 350 FAB17781D

DyLight 405 FAB17781E

DyLight 488 FAB17781K

DyLight 550 FAB17781L

DyLight 594 FAB17781M

DyLight 650 FAB17781W

DyLight 680 FAB17781Y

DyLight 755 FAB17781Z

FITC FAB17781F

HRP FAB17781H

Janelia Fluor 525 FAB17781JF525

Janelia Fluor 549 FAB17781I

Janelia Fluor 646 FAB17781J

Janelia Fluor 669 FAB17781JF669

PE/Atto594 FAB17781PEATT594

PE/Cy5.5 FAB17781PECY55

PE/Cy7 FAB17781PECY7

PerCP FAB17781C

Phycoerythrin FAB17781P

mFluor Violet 450 SE FAB17781MFV450

mFluor Violet 500 SE FAB17781MFV500

mFluor Violet 610 SE FAB17781MFV610

Catalog# & Formulation	Size	Price
Carrier Free FAB17781P

Carrier Free MAB17781-SP

Carrier Free FAB17781V-100UG

Carrier Free FAB17781U-100UG

Carrier Free FAB17781T-100UG

Carrier Free FAB17781S-100UG

Carrier Free FAB17781R-100UG

Carrier Free FAB17781N-100UG

Carrier Free FAB17781G-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB17781U-100UG
Alexa Fluor 405	FAB17781V-100UG
Alexa Fluor 488	FAB17781G-100UG
Alexa Fluor 532	FAB17781X
Alexa Fluor 594	FAB17781T-100UG
Alexa Fluor 647	FAB17781R-100UG
Alexa Fluor 700	FAB17781N-100UG
Alexa Fluor 750	FAB17781S-100UG
Allophycocyanin	FAB17781A
Allophycocyanin/Cy7	FAB17781APCCY7
Biotin	FAB17781B
CoraFluor 1	FAB17781CL1
DyLight 350	FAB17781D
DyLight 405	FAB17781E
DyLight 488	FAB17781K
DyLight 550	FAB17781L
DyLight 594	FAB17781M
DyLight 650	FAB17781W
DyLight 680	FAB17781Y
DyLight 755	FAB17781Z
FITC	FAB17781F
HRP	FAB17781H
Janelia Fluor 525	FAB17781JF525
Janelia Fluor 549	FAB17781I
Janelia Fluor 646	FAB17781J
Janelia Fluor 669	FAB17781JF669
PE/Atto594	FAB17781PEATT594
PE/Cy5.5	FAB17781PECY55
PE/Cy7	FAB17781PECY7
PerCP	FAB17781C
Phycoerythrin	FAB17781P
mFluor Violet 450 SE	FAB17781MFV450
mFluor Violet 500 SE	FAB17781MFV500
mFluor Violet 610 SE	FAB17781MFV610

Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]

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Publications for Integrin beta 1/CD29 Antibody (MAB17781)(35)

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Immunogen	Human skin keratinocytes
Specificity	Detects human Integrin beta 1/CD29.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ITGB1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Publications using MAB17781	Applications	Species
MacLeod, G;Molaei, F;Haider, S;Almeida, MP;Lin, S;Kushida, M;Sureshkumar, H;Bhatti, JK;Lu, JQ;Schramek, D;Dirks, PB;Angers, S; Fitness Screens Map State-Specific Glioblastoma Stem Cell Vulnerabilities Cancer research 2024-08-26 [PMID: 39186687] (Bioassay, Human)	Bioassay	Human
Hovhannisyan, P;Stelzner, K;Keicher, M;Paprotka, K;Neyazi, M;Pauzuolis, M;Ali, WM;Rajeeve, K;Bartfeld, S;Rudel, T; Infection of human organoids supports an intestinal niche for Chlamydia trachomatis PLoS pathogens 2024-08-01 [PMID: 39172739] (Western Blot, Human)	Western Blot	Human
Hussain, MT;Austin-Williams, S;Wright, TD;Dhawan, UK;Pinto, AL;Cooper, D;Norling, LV; ?1-Integrin-Mediated Uptake of Chondrocyte Extracellular Vesicles Regulates Chondrocyte Homeostasis International journal of molecular sciences 2024-04-26 [PMID: 38731975] (Flow Cytometery, Human)	Flow Cytometery	Human
Brooks S, Mittler S, Hamilton DW Contact Guidance of Connective Tissue Fibroblasts on Submicrometer Anisotropic Topographical Cues Is Dependent on Tissue of Origin, ?1 Integrins, and Tensin-1 Recruitment ACS applied materials & interfaces 4/26/2023 [PMID: 37067372]
SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs Nature Immunology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456734] (Neutralization, Human)	Neutralization	Human
G Liu, R Liu, Y Shan, C Sun Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I The Journal of Biological Chemistry, 2021-08-28;0(0):101133. 2021-08-28 [PMID: 34461092] (Neutralization, Human)	Neutralization	Human
Qiong Li, Lei Huang MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/ beta -Catenin Pathway International Journal of Stem Cells 11/30/2021 [PMID: 34456188]
PK Myint, A Ito, MG Appiah, G Obeng, S Darkwah, E Kawamoto, A Gaowa, EJ Park, M Shimaoka Irisin supports integrin-mediated cell adhesion of lymphocytes Biochemistry and Biophysics Reports, 2021-03-08;26(0):100977. 2021-03-08 [PMID: 33732908] (Flow Cytometry, Neutralization, Human)	Flow Cytometry, Neutralization	Human
E Kawamoto, N Nago, T Okamoto, A Gaowa, A Masui-Ito, Y Akama, S Darkwah, MG Appiah, PK Myint, G Obeng, A Ito, S Caidengbat, R Esumi, T Yamaguchi, EJ Park, H Imai, M Shimaoka The Lectin-Like Domain of Thrombomodulin Inhibits &beta1 Integrin-Dependent Binding of Human Breast Cancer-Derived Cell Lines to Fibronectin Biomedicines, 2021-02-07;9(2):. 2021-02-07 [PMID: 33562346] (Flow Cytometry, Human)	Flow Cytometry	Human
S Yuan, C Zhang, Y Zhu, B Wang Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by Inhibiting the Histone Modification of lncRNA HOTAIR Drug Design, Development and Therapy, 2020-12-07;14(0):5419-5430. 2020-12-07 [PMID: 33324039] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
G Luthria, R Li, S Wang, M Prytyskach, RH Kohler, DA Lauffenbur, TJ Mitchison, R Weissleder, MA Miller In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells Nat Commun, 2020-07-14;11(1):3521. 2020-07-14 [PMID: 32665556] (Bioassay, Human)	Bioassay	Human
SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process Cell Rep, 2019-11-05;29(6):1610-1620.e4. 2019-11-05 [PMID: 31693899] (IF, Human)	IF	Human
A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells Sci Rep, 2018-07-03;8(1):10019. 2018-07-03 [PMID: 29968781] (Flow Cytometry)	Flow Cytometry
Maciej T. Luczynski, Peter T. Harrison, Nadia Lima, Lukas Krasny, Angela Paul, Paul H. Huang Spatial localisation of Discoidin Domain Receptor 2 (DDR2) signalling is dependent on its collagen binding and kinase activity Biochemical and Biophysical Research Communications 6/18/2018 [PMID: 29709482]
SF Ahmad, JK Brown, LL Campbell, M Koscielnia, C Oliver, N Wheelhouse, G Entrican, S McFee, GS Wills, MO McClure, PJ Horner, S Gaikoumelo, KF Lee, HOD Critchley, WC Duncan, AW Horne Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin ?1 to Promote Embryo-tubal Attachment EBioMedicine, 2018-02-23;0(0):. 2018-02-23 [PMID: 29500127] (Neutralization, Human)	Neutralization	Human
CJ Lovitt, TB Shelper, VM Avery Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins BMC Cancer, 2018-01-06;18(1):41. 2018-01-06 [PMID: 29304770] (Neutralization, Human)	Neutralization	Human
Stephanie R. Gillespie, Liana J. Tedesco, Lingyan Wang, Audrey M. Bernstein The deubiquitinase USP10 regulates integrin beta1 and beta5 and fibrotic wound healing Journal of Cell Science 10/15/2017 [PMID: 28851806]
S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg Fibronectin-guided migration of carcinoma collectives Nat Commun, 2017-01-19;8(0):14105. 2017-01-19 [PMID: 28102238] (Neutralization, Human)	Neutralization	Human
Rickard J Storm Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells J. Virol, 2017-02-14;0(0):. 2017-02-14 [PMID: 27974569] (Flow Cytometry, IHC-Fr, Neutralization, Human)	Flow Cytometry, IHC-Fr, Neutralization	Human
R Bass Identification of novel peptide motifs in the serpin maspin that affect vascular smooth muscle cell function Biochim. Biophys. Acta, 2016-11-23;1864(2):336-344. 2016-11-23 [PMID: 27888098] (Neutralization, Human)	Neutralization	Human
CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes Sci Rep, 2016-09-27;6(0):34310. 2016-09-27 [PMID: 27670158] (Neutralization, Human)	Neutralization	Human
Virginia Llopis-Hernández, Marco Cantini, Cristina González-García, Zhe A. Cheng, Jingli Yang, Penelope M Tsimbouri et al. Material-driven fibronectin assembly for high-efficiency presentation of growth factors Science Advances 8/26/2016 [PMID: 27574702]
Soon Young Park, Yuji Piao, Kang Jin Jeong, Jianwen Dong, John F. de Groot Periostin (POSTN) Regulates Tumor Resistance to Antiangiogenic Therapy in Glioma Models Molecular Cancer Therapeutics 9/1/2016 [PMID: 27307601]
Jing Bai, Ting-Yuan Tu, Choong Kim, Jean Paul Thiery, Roger D. Kamm Identification of drugs as single agents or in combination to prevent carcinoma dissemination in a microfluidic 3D environment Oncotarget 11/3/2015 [PMID: 26474384]
Zhang , Qian, Dove , Christop, Hor , Jyh Lian, Murdock , Heardley, Strauss-Albee , Dara M, Garcia , Jordan A, Mandl , Judith N, Grodick , Rachael, Jing , Huie, Chandler-Brown , Devon B, Lenardo , Timothy, Crawford , Greg, Matthews , Helen F, Freeman , Alexandr, Cornall , Richard, Germain , Ronald N, Mueller , Scott N, Su , Helen C DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity. J Exp Med, 2014-11-24;211(13):2549-66. 2014-11-24 [PMID: 25422492] (Blocking, Human)	Blocking	Human
Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, Arnaout M The alpha-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in beta3 integrins. J Biol Chem, 2014-06-28;289(33):23256-63. 2014-06-28 [PMID: 24975416] (Neutralization, Human)	Neutralization	Human
Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Cell Commun Signal, 2014-06-25;12(0):36. 2014-06-25 [PMID: 24965524] (Neutralization, Human)	Neutralization	Human
Numata R, Okumura N, Nakahara M, Ueno M, Kinoshita S, Kanematsu D, Kanemura Y, Sasai Y, Koizumi N Cultivation of corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells. PLoS ONE, 2014-02-05;9(2):e88169. 2014-02-05 [PMID: 24505413] (Neutralization, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))	Neutralization	Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J, 2013-09-15;454(3):501-13. 2013-09-15 [PMID: 23822953] (Neutralization, Human)	Neutralization	Human
Calvin Chu, Emrah Celik, Felix Rico, Vincent T. Moy Elongated Membrane Tethers, Individually Anchored by High Affinity alpha 4 beta 1/VCAM-1 Complexes, Are the Quantal Units of Monocyte Arrests PLoS ONE 5/17/2013 [PMID: 23691169]
Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly. PLoS ONE, 2012-03-21;7(3):e33915. 2012-03-21 [PMID: 22470492] (Flow Cytometry, Human)	Flow Cytometry	Human
Shannon-Lowe C, Rowe M Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection. PLoS Pathog., 2011-05-05;7(5):e1001338. 2011-05-05 [PMID: 21573183] (ICC, Neutralization, Human)	ICC, Neutralization	Human
Li MQ, Hou XF, Shao J, Tang CL, Li DJ The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy. Biol. Reprod., 2010-01-14;82(5):968-79. 2010-01-14 [PMID: 20075392] (Neutralization, Human)	Neutralization	Human
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009-07-30;51(4):734-49. 2009-07-30 [PMID: 19703720] (IHC, Human)	IHC	Human
Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma. Cancer Res., 2008-11-01;68(21):9060-9. 2008-11-01 [PMID: 18974152] (ICC, Neutralization, Human)	ICC, Neutralization	Human
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