Integrin beta 1/CD29 Antibody (P5D2) [Alexa Fluor® 700] Summary
Immunogen |
Human skin keratinocytes |
Specificity |
Detects human Integrin beta 1/CD29. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
ITGB1 |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative |
0.09% Sodium Azide |
Concentration |
Please see the vial label for concentration. If unlisted please contact technical services. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Integrin beta 1/CD29 Antibody (P5D2) [Alexa Fluor® 700]
Background
The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.
- Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
- Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for Integrin beta 1/CD29 Antibody (FAB17781N). (Showing 1 - 1 of 1 FAQ).
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I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
- I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.