I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody (P5D2) [mFluor Violet 500 SE]
Brand: Novus Biologicals
SKU: FAB17781MFV500
Price: 589 USD

Question

Accepted Answer

I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, IP, B/N, CyTOF-ready, ICC/IF, KO
Clone	P5D2
Clonality	Monoclonal
Host	Mouse
Conjugate	mFluor Violet 500 SE

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB17781U-100UG

Alexa Fluor 405 FAB17781V-100UG

Alexa Fluor 488 FAB17781G-100UG

Alexa Fluor 532 FAB17781X

Alexa Fluor 594 FAB17781T-100UG

Alexa Fluor 647 FAB17781R-100UG

Alexa Fluor 700 FAB17781N-100UG

Alexa Fluor 750 FAB17781S-100UG

Allophycocyanin FAB17781A

Allophycocyanin/Cy7 FAB17781APCCY7

Biotin FAB17781B

CoraFluor 1 FAB17781CL1

DyLight 350 FAB17781D

DyLight 405 FAB17781E

DyLight 488 FAB17781K

DyLight 550 FAB17781L

DyLight 594 FAB17781M

DyLight 650 FAB17781W

DyLight 680 FAB17781Y

DyLight 755 FAB17781Z

FITC FAB17781F

HRP FAB17781H

Janelia Fluor 525 FAB17781JF525

Janelia Fluor 549 FAB17781I

Janelia Fluor 646 FAB17781J

Janelia Fluor 669 FAB17781JF669

PE/Atto594 FAB17781PEATT594

PE/Cy5.5 FAB17781PECY55

PE/Cy7 FAB17781PECY7

PerCP FAB17781C

Phycoerythrin FAB17781P

Unconjugated MAB17781

mFluor Violet 450 SE FAB17781MFV450

mFluor Violet 610 SE FAB17781MFV610

Catalog# & Formulation	Size	Price
Standard Formulation MAB17781

Carrier Free MAB17781

Carrier Free FAB17781P

Carrier Free MAB17781-SP

Carrier Free FAB17781V-100UG

Carrier Free FAB17781U-100UG

Carrier Free FAB17781T-100UG

Carrier Free FAB17781S-100UG

Carrier Free FAB17781R-100UG

Carrier Free FAB17781N-100UG

Carrier Free FAB17781G-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB17781U-100UG
Alexa Fluor 405	FAB17781V-100UG
Alexa Fluor 488	FAB17781G-100UG
Alexa Fluor 532	FAB17781X
Alexa Fluor 594	FAB17781T-100UG
Alexa Fluor 647	FAB17781R-100UG
Alexa Fluor 700	FAB17781N-100UG
Alexa Fluor 750	FAB17781S-100UG
Allophycocyanin	FAB17781A
Allophycocyanin/Cy7	FAB17781APCCY7
Biotin	FAB17781B
CoraFluor 1	FAB17781CL1
DyLight 350	FAB17781D
DyLight 405	FAB17781E
DyLight 488	FAB17781K
DyLight 550	FAB17781L
DyLight 594	FAB17781M
DyLight 650	FAB17781W
DyLight 680	FAB17781Y
DyLight 755	FAB17781Z
FITC	FAB17781F
HRP	FAB17781H
Janelia Fluor 525	FAB17781JF525
Janelia Fluor 549	FAB17781I
Janelia Fluor 646	FAB17781J
Janelia Fluor 669	FAB17781JF669
PE/Atto594	FAB17781PEATT594
PE/Cy5.5	FAB17781PECY55
PE/Cy7	FAB17781PECY7
PerCP	FAB17781C
Phycoerythrin	FAB17781P
Unconjugated	MAB17781
mFluor Violet 450 SE	FAB17781MFV450
mFluor Violet 610 SE	FAB17781MFV610

Integrin beta 1/CD29 Antibody (P5D2) [mFluor Violet 500 SE]

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Bioinformatics

Immunogen	Human skin keratinocytes
Specificity	Detects human Integrin beta 1/CD29.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ITGB1
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Blockade of Receptor-ligand Interaction CyTOF-ready Flow Cytometry Immunocytochemistry Immunoprecipitation Knockout Validated
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide