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Integrin alpha L/CD11a Antibody (345913) [Unconjugated]

 (3 Publications)
 (1 Review)
 
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Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin aL/CD11a at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of PBMC, Placenta, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 220 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clone
345913
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3595U-100UG
Alexa Fluor 405
FAB3595V-100UG
Alexa Fluor 488
FAB3595G-100UG
Alexa Fluor 532
FAB3595X
Alexa Fluor 594
FAB3595T-100UG
Alexa Fluor 647
FAB3595R-100UG
Alexa Fluor 700
FAB3595N-100UG
Alexa Fluor 750
FAB3595S-100UG
Allophycocyanin/Cy7
FAB3595APCCY7
Biotin
FAB3595B
CoraFluor 1
FAB3595CL1
DyLight 350
FAB3595D
DyLight 405
FAB3595E
DyLight 488
FAB3595K
DyLight 550
FAB3595L
DyLight 594
FAB3595M
DyLight 650
FAB3595W
DyLight 680
FAB3595Y
DyLight 755
FAB3595Z
Fluorescein
FAB3595F
HRP
FAB3595H
Janelia Fluor 525
FAB3595JF525
Janelia Fluor 549
FAB3595I
Janelia Fluor 646
FAB3595J
Janelia Fluor 669
FAB3595JF669
PE/Atto594
FAB3595PEATT594
PE/Cy5.5
FAB3595PECY55
PE/Cy7
FAB3595PECY7
PerCP
FAB3595C
mFluor Violet 450 SE
FAB3595MFV450
mFluor Violet 500 SE
FAB3595MFV500
mFluor Violet 610 SE
FAB3595MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB3595F
Carrier Free
MAB3595-SP
Carrier Free
FAB3595T-100UG
Carrier Free
FAB3595S-100UG
Carrier Free
FAB3595R-100UG
Carrier Free
FAB3595N-100UG
Carrier Free
FAB3595G-100UG
Carrier Free
FAB3595V-100UG
Carrier Free
FAB3595U-100UG
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Integrin alpha L/CD11a Antibody (345913) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha L
Tyr26-Met1089 (Tyr660Ile)
Accession # CAA68747
Specificity
Detects human Integrin alpha L in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB3595 in the following applications:
Publications
Read Publications using
MAB3595 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha L/CD11a Antibody (345913) [Unconjugated]

  • CD11 antigen-like family member A
  • CD11a antigen
  • CD11a
  • CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
  • Integrin alpha L
  • integrin alpha-L
  • integrin gene promoter
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
  • ITGAL
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • Leukocyte function-associated molecule 1 alpha chain
  • LFA-1 alpha
  • LFA-1
  • LFA1A
  • LFA-1A
  • Ly-15
  • Ly-21
  • lymphocyte function-associated antigen 1

Background

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Integrin alpha
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MAB3595
Integrin alpha L/CD11a Antibody (345913) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, Flow, CyTOF-ready

Publications for Integrin alpha L/CD11a Antibody (MAB3595)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


Filter By Application
Neutralization
(2)
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Human
(2)
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Review for Integrin alpha L/CD11a Antibody (MAB3595) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB3595:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Human 02/20/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20810987
SpeciesHuman

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WB Video Protocol

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Recent Reviews

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4
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1
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 Verified Customer
02/20/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol ITGAL
Uniprot