Home » L-Selectin/CD62L » L-Selectin/CD62L Antibodies » L-Selectin/CD62L Antibody (4G8) [Unconjugated]

L-Selectin/CD62L Antibody (4G8) [Unconjugated]

 (9 Publications)
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PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) or (B) isotype control antibody (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ELISA(Cap)
Clone
4G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9787U-100UG
Alexa Fluor 405
FAB9787V-100UG
Alexa Fluor 488
FAB9787G-100
Alexa Fluor 594
FAB9787T-100
Alexa Fluor 647
FAB9787R-100
Alexa Fluor 700
FAB9787N-100UG
Alexa Fluor 750
FAB9787S-100UG
Phycoerythrin
FAB9787P-100
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Carrier Free
FAB9787P-100
Carrier Free
FAB9787U-100UG
Carrier Free
FAB9787V-100UG
Carrier Free
FAB9787N-100UG
Carrier Free
FAB9787S-100UG
Carrier Free
FAB9787G-100
Carrier Free
FAB9787R-100
Carrier Free
FAB9787T-100
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L-Selectin/CD62L Antibody (4G8) [Unconjugated] Summary

Immunogen
Recombinant human L‑Selectin/CD62L
Extracellular domain
Specificity
Binds to CHO cells expressing the human L‑Selectin/CD62L protein. It does not recognize CHO cells expressing either the human E-Selectin or human P-Selectin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
Application Notes
ELISA Capture: Human L-Selectin/CD62L Antibody (Catalog # BBA24)
ELISA Detection: Human L-Selectin/CD62L Biotinylated Antibody (Catalog # BAF728)
Standard: Recombinant Human L-Selectin/CD62L (Catalog # ADP2)
Publications
Read Publications using
BBA24 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for L-Selectin/CD62L Antibody (4G8) [Unconjugated]

  • CD62L antigen
  • CD62L
  • gp90-MEL
  • hLHRc
  • LAM1
  • LAM-1
  • LAM1LECAM1
  • LECAM1
  • LEU8
  • Leu-8
  • Leukocyte adhesion molecule 1
  • Leukocyte surface antigen Leu-8
  • Leukocyte-endothelial cell adhesion molecule 1
  • LNHR
  • LNHRTQ1
  • LSEL
  • L-Selectin
  • LYAM1
  • Lyam-1
  • LYAM1CD62 antigen-like family member L
  • Lymph node homing receptor
  • lymphocyte adhesion molecule 1
  • pln homing receptor
  • PLNHR
  • selectin L
  • SELL
  • TQ1

Background

L-Selectin (LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. It plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for L-Selectin/CD62L Antibody (BBA24)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, Flow Cytometry.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(5)
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Human
(6)
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Showing Publications 1 - 9 of 9.
Publications using BBA24 Applications Species
Gayatri S. Phadke, Jennifer E. Satterwhite-Warden, Dharamainder Choudhary, John A. Taylor, James F. Rusling A novel and accurate microfluidic assay of CD62L in bladder cancer serum samples The Analyst 11/5/2018 [PMID: 30295303]
ND Barth, JA Marwick, MJ Heeb, AJ Gale, AG Rossi, I Dransfield Augmentation of Human Monocyte Responses to Lipopolysaccharide by the Protein S and Mer/Tyro3 Receptor Tyrosine Kinase Axis J. Immunol., 2018-09-24;0(0):. 2018-09-24 [PMID: 30249810]
Vassilios Lougaris NFKB1 regulates human NK cell maturation and effector functions Clin. Immunol, 2016-12-03;0(0):. 2016-12-03 [PMID: 27923702] (Flow Cytometry, Human) Flow Cytometry Human
Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation. Scientific Reports, 2016-02-26;0(0):2045-2322. 2016-02-26 [PMID: 26915707] (Flow Cytometry, Human) Flow Cytometry Human
Chang , Elizabet, Eckert , Mark A, Ali , M Monsur, Riazifar , Hamidrez, Pone , Egest J, Liu , Linan, Zhao , Weian Facile supermolecular aptamer inhibitors of L-selectin. PLoS ONE, 2015-03-31;10(3):e0123034. 2015-03-31 [PMID: 25826688] (ELISA Development, Human) ELISA Development Human
Robert W. Yucha, Monika Jost, Dianne Rothstein, Noreen Robertson, Michele S. Marcolongo Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres” Tissue Engineering Part A 1/1/2014 [PMID: 23927766]
Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. J. Immunol., 2011-12-16;188(3):1002-10. 2011-12-16 [PMID: 22180616] (Flow Cytometry, Human) Flow Cytometry Human
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human) Flow Cytometry Human
Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action. Immunity, 2003-01-01;18(1):87-96. 2003-01-01 [PMID: 12530978] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

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Blogs on L-Selectin/CD62L.

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L-selectin (CD62L antigen, Leukocyte surface antigen Leu-8)
L-selectin is a member of the selectin family of glycoprotein adhesion and homing receptors that recognize sialyated carbohydrate groups and regulate lymphocyte-endothelial cell interactions. It is a type I transmembrane cell adhesion molecule (CAM) a...  Read full blog post.
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Bioinformatics

Gene Symbol SELL