Name: E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
Brand: Novus Biologicals
SKU: BBA16
Rating: 5 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, IHC, IP, ELISA(Cap), ICC/IF
Clone	BBIG-E4
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Additional Information	Clone BBIG-E4 was used by HLDA to establish CD designation
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Specificity	Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	SELE
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Sterile PBS to a final concentration of 0.5 mg/mL.

Publications using BBA16	Applications	Species
T Pirker, EM Pferschy-W, E Bampali, V Bochkov, R Bauer Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8 Journal of ethnopharmacology, 2023-03-02;0(0):116328. 2023-03-02 [PMID: 36870464] (BBA16, Human)	BBA16	Human
Giovanni S. Offeddu, Cynthia Hajal, Colleen R. Foley, Zhengpeng Wan, Lina Ibrahim, Mark F. Coughlin et al. The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination Communications Biology 2021-02-26 [PMID: 33637851]
Dafeng Yang, Stefan Haemmig, Haoyang Zhou, Daniel Pérez-Cremades, Xinghui Sun, Lei Chen et al. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway eLife 2021-01-08 [PMID: 33416495]
Hiten D. Mistry, Melissa V. Hott Ogalde, Fiona Broughton Pipkin, Geneviève Escher, Lesia O. Kurlak Maternal, Fetal, and Placental Selectins in Women With Pre-eclampsia; Association With the Renin-Angiotensin-System Frontiers in Medicine 2020-06-12 [PMID: 32596247]
S Mylvaganam, M Riedl, A Vega, RF Collins, K Jaqaman, S Grinstein, SA Freeman Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes Cell Rep, 2020-06-23;31(12):107798. 2020-06-23 [PMID: 32579925] (ICC, Human)	ICC	Human
Nora M. Schacher, Dorette Raaz‑Schrauder, Francesca Pasutto, Florian M. Stumpfe, Miyuki Tauchi, Barbara Dietel et al. Impact of single nucleotide polymorphisms in the VEGFR2 gene on endothelial cell activation under non‑uniform shear stress International Journal of Molecular Medicine 2019-08-05 [PMID: 31432097]
J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling Front Pharmacol, 2019-04-26;10(0):289. 2019-04-26 [PMID: 31105555] (ELISA - Cell Based, Human)	ELISA - Cell Based	Human
Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li Pinocembrin protects endothelial cells from oxidized LDL-induced injury Cytokine, 2018-06-18;0(0):. 2018-06-18 [PMID: 29914794] (Western Blot, Human)	Western Blot	Human
R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer A modular platform for targeted RNAi therapeutics Nat Nanotechnol, 2018-01-29;0(0):. 2018-01-29 [PMID: 29379205]
C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018-01-11;8(1):420. 2018-01-11 [PMID: 29323143] (Western Blot, Human)	Western Blot	Human
BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer Oncogene, 2017-12-18;0(0):. 2017-12-18 [PMID: 29249802]
H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death Cell Death Differ., 2017-12-12;0(0):. 2017-12-12 [PMID: 29234155]
S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers Infect. Immun., 2018-02-20;0(0):. 2018-02-20 [PMID: 29203545]
H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both inï¿½vivo and inï¿½vitro Mol Med Rep, 2017-10-25;0(0):. 2017-10-25 [PMID: 29115443]
L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity Inï¿½Vivo Mol. Ther., 2017-10-13;0(0):. 2017-10-13 [PMID: 29103909]
K Yan, Y Shen Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system Mol Med Rep, 2017-07-28;0(0):. 2017-07-28 [PMID: 28765966]
A Tang, C Li, Z Chen, T Li Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ Tï¿½cells and T-bet/GATA-3 expression in NOD mice Mol Med Rep, 2017-07-28;0(0):. 2017-07-28 [PMID: 28765956]
S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education Scand. J. Immunol., 2017-09-01;0(0):. 2017-09-01 [PMID: 28605050]
X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation Allergy, 2017-07-03;0(0):. 2017-07-03 [PMID: 28580587]
JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis Transl Psychiatry, 2017-05-30;7(5):e1138. 2017-05-30 [PMID: 28556833]
W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells Mol. Pharmacol, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280061]
F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi Amine oxidase activity regulates the development of pulmonary fibrosis FASEB J, 2017-03-01;0(0):. 2017-03-01 [PMID: 28251930]
J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model Inflammopharmacology, 2017-03-01;0(0):. 2017-03-01 [PMID: 28251487]
YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine J. Cancer Res. Clin. Oncol, 2017-02-25;0(0):. 2017-02-25 [PMID: 28238064]
JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment Int J Mol Sci, 2017-02-14;18(2):. 2017-02-14 [PMID: 28216566]
K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis Respirology, 2017-02-14;0(0):. 2017-02-14 [PMID: 28198072]
Bernhard Wernly Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI) Eur. J. Clin. Invest, 2017-02-01;0(0):. 2017-02-01 [PMID: 28036122]
Xuejun Cheng Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture Dev Neurobiol, 2017-03-23;0(0):. 2017-03-23 [PMID: 28033654]
Gong-Jhe Wu Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway J. Cell. Biochem, 2017-05-16;0(0):. 2017-05-16 [PMID: 27987330]
Lin Li IL-17RB(+) granulocytes are associated with airflow obstruction in asthma Ann. Allergy Asthma Immunol, 2016-12-01;117(6):674-679. 2016-12-01 [PMID: 27979026]
Tra M T Bui Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles Reprod. Fertil. Dev, 2017-09-01;0(0):. 2017-09-01 [PMID: 27938625]
Minu Karthika Ganesan 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells Tissue Eng Part C Methods, 2017-01-01;0(0):. 2017-01-01 [PMID: 27923320] (ICC, Human)	ICC	Human
Dimitrios Agas INF-? encoding plasmid administration triggers bone loss and disrupts bone marrow microenvironment J. Endocrinol, 2016-12-01;0(0):. 2016-12-01 [PMID: 27908965]
Trevor R F Smith Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin Vaccine, 2016-11-25;35(1):61-70. 2016-11-25 [PMID: 27894716]
Zhonghao Liu Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats Can J Diabetes, 2016-11-23;0(0):. 2016-11-23 [PMID: 27889175]
Jun Tao BMP4/Id2 signaling pathway is a novel therapeutic target for late outgrowth endothelial progenitor cell-mediated endothelial injury repair Int. J. Cardiol., 2016-11-09;228(0):796-804. 2016-11-09 [PMID: 27888757]
Georgi Guruli Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model Prostate, 2016-11-08;0(0):. 2016-11-08 [PMID: 27862100]
Simon X Liang Proliferation, differentiation and migration of SCA1(-)/CD31(-) cardiac side population cells in vitro and in vivo Int. J. Cardiol., 2016-11-09;0(0):. 2016-11-09 [PMID: 27847151]
Xiaoye Yan Neutrophil gelatinase-associated lipocalin predicts myocardial dysfunction and mortality in severe sepsis and septic shock Int. J. Cardiol., 2016-11-03;0(0):. 2016-11-03 [PMID: 27836304]
Bruno Rodrigues Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats Int. J. Cardiol., 2016-10-26;0(0):. 2016-10-26 [PMID: 27823896]
Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis J Cell Mol Med, 2016-08-25;0(0):. 2016-08-25 [PMID: 27558380]
C13orf31 (FAMIN) is a central regulator of immunometabolic function Nat Immunol, 2016-08-01;0(0):. 2016-08-01 [PMID: 27478939]
Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress Am J Hypertens, 2016-11-01;0(0):. 2016-11-01 [PMID: 27465439]
Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B Immunology, 2016-08-24;0(0):. 2016-08-24 [PMID: 27441725]
Christoph Wilhelm, Oliver J Harrison, Vanessa Schmitt, Martin Pelletier, Sean P Spencer, Joseph F Urban, Michelle Ploch, Thirumalai R Ramalinga, Richard M Siegel, Yasmine Belkaid Critical role of fatty acid metabolism in ILC2-mediated barrier protection during malnutrition and helminth infection. The Journal of Experimental Medicine, 2016-07-18;0(0):1540-9538. 2016-07-18 [PMID: 27432938]
Paul M. Holloway, Scarlett Gillespie, Felix Becker, Shantel A. Vital, Victoria Nguyen, J. Steven Alexander et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways Vascular Pharmacology 2016-10-01 [PMID: 27401964]
ME Legrier, I BiŠche, J Gaston, A Beurdeley, V Yvonnet, O D‚as, A Thuleau, S Chƒteau-Jo, JL Servely, S Vacher, M Lassalle, S Depil, GC Tucker, JJ Fontaine, MF Poupon, S Roman-Roma, JG Judde, D Decaudin, S Cairo, E Marangoni Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer Br. J. Cancer, 2015-12-22;114(2):177-87. 2015-12-22 [PMID: 26695443]
Wang F, Hou H, Wu S, Tang Q, Liu W, Huang M, Yin B, Huang J, Mao L, Lu Y, Sun Z TIGIT expression levels on human NK cells correlate with functional heterogeneity among healthy individuals. Eur J Immunol, 2015-09-07;45(10):2886-97. 2015-09-07 [PMID: 26171588]
Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis. Mol Ther, 2015-03-27;23(6):1044-54. 2015-03-27 [PMID: 25815697]
Marion Avril, Andrew J. Brazier, Martin Melcher, Sowmya Sampath, Joseph D. Smith DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells PLoS Pathogens 2013-06-27 [PMID: 23825944]
Pezeshkian , Bahareh, Donnelly , Christop, Tamburo , Kelley, Geddes , Timothy, Madlambayan , Gerard J Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism. PLoS ONE, 2013-04-01;8(4):e60823. 2013-04-01 [PMID: 23560111] (Functional Assay, Human)	Functional Assay	Human
Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Lab. Invest., 2007-09-17;87(11):1159-70. 2007-09-17 [PMID: 17876298] (ICC, Human)	ICC	Human
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (ICC, Human)	ICC	Human
Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Dev. Biol., 2006-06-15;298(1):107-17. 2006-06-15 [PMID: 16930583] (IHC-Fr, Human)	IHC-Fr	Human
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006-08-01;108(3):928-35. 2006-08-01 [PMID: 16861346] (ELISA Development, Human)	ELISA Development	Human
Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Blood, 2005-11-29;107(6):2252-61. 2005-11-29 [PMID: 16317091] (Neutralization, Human)	Neutralization	Human
Armitage JD, Lindsey NJ, Homer-Vanniasinkam S The role of endothelial cell reactive antibodies in peripheral arterial disease. Eur J Vasc Endovasc Surg, 2005-10-14;31(2):170-5. 2005-10-14 [PMID: 16226905] (ELISA Development, Human)	ELISA Development	Human
Frattini J, Kudo FA Differential effects of orbital and laminar shear stress on endothelial cells. J. Vasc. Surg., 2005-05-01;41(5):869-80. 2005-05-01 [PMID: 15886673] (ICC, Human)	ICC	Human
Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6. 2005-02-16 [PMID: 15716293] (IHC, Human)	IHC	Human
Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction. J. Vasc. Surg., 2004-03-01;39(3):613-20. 2004-03-01 [PMID: 14981456] (Flow Cytometry, Human)	Flow Cytometry	Human
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E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]

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	Verified Customer 11/28/2021
Application:	IHC-P
Species:	Human