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CD31/PECAM-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) ...read more
Simple Western shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 165 kDa (as indicated) using 10 µg/mL of Goat ...read more
Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (AB-108-C, open histogram), followed by ...read more
Rat splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), ...read more
CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more
HUVEC cells were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (AF3628) ...read more
Representative image of immunostaining corresponding to the glucose transporter protein SLC2A1 (GLUT1) in 30 week-old Ins2AKITA and WT cerebral cortex. Mural cells (ANPEP, green), glucose transporter 1 (GLUT1, red), ...read more
Biological Strategies: AcSDKP suppresses TGF beta /smad signaling and EndMT through the FGFR1/FRS2 pathway. (a) HMVECs were treated with N-FGFR1 for 48 h, and the FGFR1, TGF beta R1 and TGF beta R2 protein ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
Biological Strategies: AcSDKP suppresses TGF beta /smad signaling and EndMT through the FGFR1/FRS2 pathway. (a) HMVECs were treated with N-FGFR1 for 48 h, and the FGFR1, TGF beta R1 and TGF beta R2 protein ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious ...read more
Blood-brain barrier permeability measurements in male Ins2AKITA and WT littermate controls. (a) Representative stereomicroscope fluorescence images of brains showing 1 kDa Alexa Fluor 555 cadaverine permeability in ...read more
Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
CL and low temperature lead to WAT browning, angiogenesis, and an increased number of myofibroblast-like cells. a, b Histological analysis of adipocyte morphology (H&E), adipocytes (PERI), mitochondria (COX4), ...read more
Neutrophil depletion reduces BBB breakdown and increases neovascularization after stroke. f, g Representative confocal images (f) and quantitative analysis of IgG extravascular deposits (g) in the peri-infarct cortex at ...read more
Increased vascular remodeling by delayed inhibition of NET formation.a–d Representative confocal images (a, c) and quantitative analysis of IgG extravascular deposits (b, d) in the peri-infarct cortex at 14 days. Mice ...read more
Increased vascular remodeling by delayed inhibition of NET formation.a–d Representative confocal images (a, c) and quantitative analysis of IgG extravascular deposits (b, d) in the peri-infarct cortex at 14 days. Mice ...read more
Neutrophil depletion reduces BBB breakdown and increases neovascularization after stroke. h, j Representative confocal images (h) of CD31-positive microvessels and in-vivo multiphoton microscopy images of perfused ...read more
Increased vascular remodeling by delayed inhibition of NET formation.a–d Representative confocal images (a, c) and quantitative analysis of IgG extravascular deposits (b, d) in the peri-infarct cortex at 14 days. Mice ...read more
Neutrophils accumulate in the brain during all stages of ischemic stroke. f Representative confocal images of Ly6G-labeled neutrophils (green) and CD31-positive microvessels (white) in the peri-infarct cortex of mice at ...read more
Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious ...read more
Increased vascular remodeling by delayed inhibition of NET formation.a–d Representative confocal images (a, c) and quantitative analysis of IgG extravascular deposits (b, d) in the peri-infarct cortex at 14 days. Mice ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
   

Biological Strategies

     

CD31/PECAM-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Specificity
Detects human and mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant porcine CD31 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PECAM1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 8 Reviews rated 5
using
AF3628 in the following applications:

Publications
Read Publications using
AF3628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (AF3628)(494)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenograft.

We have publications tested in 19 applications: Cell Culture, Cell Selection, Flow Cytometry, Functional Assay, ICC, ICC/IF, IF, IF/IHC, IHC, IHC fixed frozen, IHC-Fr, IHC-P, IHC-Pr, IHC/IF, IIHC, Ifs, Immunocytochemistry, Immunohistochemistry, Western Blot.


Filter By Application
Cell Culture
(2)
Cell Selection
(1)
Flow Cytometry
(6)
Functional Assay
(1)
ICC
(9)
ICC/IF
(2)
IF
(2)
IF/IHC
(1)
IHC
(191)
IHC fixed frozen
(1)
IHC-Fr
(8)
IHC-P
(19)
IHC-Pr
(1)
IHC/IF
(3)
IIHC
(1)
Ifs
(1)
Immunocytochemistry
(1)
Immunohistochemistry
(29)
Western Blot
(8)
All Applications
Filter By Species
Human
(10)
Mouse
(214)
Rat
(36)
Transgenic Mouse
(22)
Xenograft
(4)
All Species
Showing Publications 1 - 10 of 494. Show All 494 Publications.
Publications using AF3628 Applications Species
Korte, N;Barkaway, A;Wells, J;Freitas, F;Sethi, H;Andrews, SP;Skidmore, J;Stevens, B;Attwell, D; Inhibiting Ca2+ channels in Alzheimer's disease model mice relaxes pericytes, improves cerebral blood flow and reduces immune cell stalling and hypoxia Nature neuroscience 2024-09-18 [PMID: 39294491] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Ghim, M;Wei, L;Jung, JJ;The, E;Kukreja, G;Neishabouri, A;Ahmad, AA;Raza, MZ;Golbazi, A;Hedayatyanfard, K;Nie, L;Zhang, J;Sadeghi, MM; Regulation of angiogenesis by signal sequence-derived peptides bioRxiv : the preprint server for biology 2024-08-23 [PMID: 39229053] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Cazuza, RA;Zagrai, SM;Grieco, AR;Avery, TD;Abell, AD;Wey, HY;Loggia, ML;Grace, PM; Translocator protein 18?kDa (TSPO) is upregulated in rat brain after peripheral nerve injury and downregulated by diroximel fumarate Brain, behavior, and immunity 2024-08-30 [PMID: 39218234] (Immunohistochemistry, Rat) Immunohistochemistry Rat
Perrin, S;Ethel, M;Bretegnier, V;Goachet, C;Wotawa, CA;Luka, M;Coulpier, F;Masson, C;Ménager, M;Colnot, C; Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration bioRxiv : the preprint server for biology 2024-07-26 [PMID: 39211065] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Shabani, Z;Do Prado, LB;Zhang, R;Zhu, W;Shaligram, SS;Yadav, A;Wang, C;Su, H; Increasing Endoglin Deletion in Endothelial Cells Exacerbates the Severity of Brain Arteriovenous Malformation in Mouse Biomedicines 2024-07-30 [PMID: 39200156] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Phinney, NZ;Huang, X;Toombs, JE;Brekken, RA; Development of betabodies: the next generation of phosphatidylserine targeting agents The Journal of biological chemistry 2024-08-17 [PMID: 39159812] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Zhang, Q;Xue, J;Tang, J;Wu, S;Liu, Z;Wu, C;Liu, C;Liu, Y;Lin, J;Han, J;Liu, L;Chen, Y;Yang, J;Li, Z;Zhao, L;Wei, Y;Li, Y;Zhuo, Y; Modulating amacrine cell-derived dopamine signaling promotes optic nerve regeneration and preserves visual function Science advances 2024-08-02 [PMID: 39093964] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Pathan, S;Pugazenthi, A;Dixon, BR;Wensel, TG;Rosengart, TK;Mathison, M; Activation of a GPCR, ORL1 receptor: A novel therapy to prevent heart failure progression Research square 2024-07-18 [PMID: 39070633] (Immunohistochemistry, Rat) Immunohistochemistry Rat
Werner, F;Naruke, T;Sülzenbrück, L;Schäfer, S;Rösch, M;Völker, K;Krebes, L;Abe beta er, M;Möllmann, D;Baba, HA;Schweda, F;Zernecke, A;Kuhn, M; Auto/Paracrine C-Type Natriuretic Peptide/Cyclic GMP Signaling Prevents Endothelial Dysfunction International journal of molecular sciences 2024-07-16 [PMID: 39063044] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Su, X;Liang, F;Zeng, Y;Yang, ZR;Deng, YZ;Xu, YH;Cai, XW; Radiation-Induced Endothelial Ferroptosis Accelerates Atherosclerosis via the DDHD2-Mediated Nrf2/GPX4 Pathway Biomolecules 2024-07-22 [PMID: 39062593] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Show All 494 Publications.

Reviews for CD31/PECAM-1 Antibody (AF3628) (8) 58

Average Rating: 5
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Mouse, Mouse and Mouse brain tissue.

Reviews using AF3628:
Filter by Applications
IHC-Fr
(3)
IHC-P
(1)
IHC
(1)
Simple Western
(1)
ICC
(2)
All Applications
Filter by Species
Mouse
(7)
Mouse and Mouse brain tissue
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/05/2024
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse sciatic nerve,Sciatic Nerve
SpeciesMouse
LotYZU0122121

Comments

CommentsAF3628 was diluted 1 in 500 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti goat conjugated to Alexa 594.
  5
reviewed by:
Kevin Thorburn
IHC-P Mouse 04/06/2023
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE
SpeciesMouse
LotYZU0122121
Immunohistochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse and Mouse brain tissue 05/10/2019
View

Summary

ApplicationImmunohistochemistry
Sample TestedP12 brain cortex,P21 brain cortex
SpeciesMouse and Mouse brain tissue
LotYZU0117011

Comments

CommentsBlocking with 10% donkey serum and PBST (.3%TritonX100). Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good.
Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Ricardo Vieira
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult spinal cord,Embryonic spinal cord
SpeciesMouse
LotYZU00118051
Simple Western CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
Simple Western Mouse 05/25/2018
View

Summary

ApplicationSimple Western
Sample TestedVein Lysate
SpeciesMouse
LotYZU0118011

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
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5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/02/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain
SpeciesMouse
LotYZU0116121

Comments

CommentsCD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
IgG Secondary Antibody and counterstained with DAPI (blue).
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse

Comments

CommentsFrozen Tissue
4% PFA fixation
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse
LotYZU0116121

Comments

CommentsFrozen tissue
Cold Acetone fixation

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

  1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
    • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Blogs on CD31/PECAM-1.


  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
8
4
0
3
0
2
0
1
0

Verified Customer
04/05/2024
Application: IHC-Fr
Species: Mouse

Kevin Thorburn
04/06/2023
Application: IHC-P
Species: Mouse

Verified Customer
05/10/2019
Application: IHC
Species: Mouse and Mouse brain tissue

Bioinformatics

Gene Symbol PECAM1
Uniprot