Home » IL-1 beta/IL-1F2 » IL-1 beta/IL-1F2 Proteins » Recombinant Human IL-1 beta/IL-1F2 Protein

Recombinant Human IL-1 beta/IL-1F2 Protein

 (601 Publications)
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Recombinant Human IL-1beta Protein (Catalog # 201-LB) has a molecular weight (MW) of 17.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-1beta Protein (Catalog # 201-LB) has a molecular weight (MW) of 17.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
201-LB-005/CF
Carrier Free
201-LB-025/CF
Carrier Free
201-LB-500/CF
Carrier Free
201-LB-100/CF
Carrier Free
201-LB-010/CF
Carrier Free
201-LB-01M/CF
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Recombinant Human IL-1 beta/IL-1F2 Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
201-LB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
*The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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201-LB
Recombinant Human IL-1 beta/IL-1F2 Protein
Species: Hu
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Publications for IL-1 beta/IL-1F2 (201-LB)(601)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit, Transgenic Mouse.

We have publications tested in 11 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Capture, In Vivo, Inhibition, Stimulation, Surface Plasmon Resonance, Western Blot, Western Blot Control.


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Binding Assay
(2)
Bioassay
(512)
Cell Culture
(52)
ELISA (Standard)
(13)
ELISA Capture
(4)
In Vivo
(10)
Inhibition
(1)
Stimulation
(2)
Surface Plasmon Resonance
(1)
Western Blot
(1)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(521)
Mouse
(34)
Rat
(16)
Bovine
(5)
Canine
(3)
Guinea Pig
(1)
Hamster
(1)
N/A
(2)
Porcine
(3)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 601. Show All 601 Publications.
Publications using 201-LB Applications Species
Sankaranarayanan, J;Lee, SC;Kim, HK;Kang, JY;Kuppa, SS;Seon, JK; Cinnamaldehyde-Mediated Suppression of MMP-13, COX-2, and IL-6 Through MAPK and NF-?B Signaling Inhibition in Chondrocytes and Synoviocytes Under Inflammatory Conditions International journal of molecular sciences 2024-11-30 [PMID: 39684628] (Bioassay, Human) Bioassay Human
Han, H;Lee, S;Gao, G;Yi, HG;Paek, SH;Jang, J; Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood-Brain Barrier Properties Biomaterials research 2024-12-05 [PMID: 39641002] (Bioassay, Human) Bioassay Human
Ehrenberg, PK;Geretz, A;Volcic, M;Izumi, T;Yum, L;Waickman, A;Shangguan, S;Paquin-Proulx, D;Creegan, M;Bose, M;Machmach, K;McGraw, A;Narahari, A;Currier, JR;Sacdalan, C;Phanuphak, N;Apps, R;Corley, M;Ndhlovu, LC;Slike, B;Krebs, SJ;Anonworanich, J;Tovanabutra, S;Robb, ML;Eller, MA;Laird, GM;Cyktor, J;Daar, ES;Crowell, TA;Mellors, JW;Vasan, S;Michael, NL;Kirchhoff, F;Thomas, R; Single-cell analyses reveal that monocyte gene expression profiles influence HIV-1 reservoir size in acutely treated cohorts bioRxiv : the preprint server for biology 2024-11-15 [PMID: 39605411] (Bioassay, Human) Bioassay Human
Flores-Gomez, D;Hobo, W;van Ens, D;Kessler, EL;Novakovic, B;Schaap, NPM;Rijnen, WHC;Joosten, LAB;Netea, MG;Riksen, NP;Bekkering, S; Interleukin-1? induces trained innate immunity in human hematopoietic progenitor cells in vitro Stem cell reports 2024-09-25 [PMID: 39515317] (Bioassay, Human) Bioassay Human
Bergström, B;Selldén, T;Bollmann, M;Svensson, MND;Ekwall, AH; Methotrexate promotes the release of granulocyte-macrophage colony-stimulating factor from rheumatoid arthritis fibroblast-like synoviocytes via autocrine interleukin-1 signaling Arthritis research & therapy 2024-10-11 [PMID: 39394168] (Bioassay, Human) Bioassay Human
Li, Y;Li, TY;Qiao, Q;Zhang, MT;Tong, MX;Xu, LF;Zhang, ZB; Polymeric immunoglobulin receptor promotes Th2 immune response in the liver by increasing cholangiocytes derived IL-33: a diagnostic and therapeutic biomarker of biliary atresia EBioMedicine 2024-09-16 [PMID: 39288533] (Cell Culture, Human) Cell Culture Human
Arias, AA;Neehus, AL;Ogishi, M;Meynier, V;Krebs, A;Lazarov, T;Lee, AM;Arango-Franco, CA;Yang, R;Orrego, J;Corcini Berndt, M;Rojas, J;Li, H;Rinchai, D;Erazo-Borrás, L;Han, JE;Pillay, B;Ponsin, K;Chaldebas, M;Philippot, Q;Bohlen, J;Rosain, J;Le Voyer, T;Janotte, T;Amarajeeva, K;Soudée, C;Brollo, M;Wiegmann, K;Marquant, Q;Seeleuthner, Y;Lee, D;Lainé, C;Kloos, D;Bailey, R;Bastard, P;Keating, N;Rapaport, F;Khan, T;Moncada-Vélez, M;Carmona, MC;Obando, C;Alvarez, J;Cataño, JC;Martínez-Rosado, LL;Sanchez, JP;Tejada-Giraldo, M;L'Honneur, AS;Agudelo, ML;Perez-Zapata, LJ;Arboleda, DM;Alzate, JF;Cabarcas, F;Zuluaga, A;Pelham, SJ;Ensser, A;Schmidt, M;Velásquez-Lopera, MM;Jouanguy, E;Puel, A;Krönke, M;Ghirardello, S;Borghesi, A;Pahari, S;Boisson, B;Pittaluga, S;Ma, CS;Emile, JF;Notarangelo, LD;Tangye, SG;Marr, N;Lachmann, N;Salvator, H;Schlesinger, LS;Zhang, P;Glickman, MS;Nathan, CF;Geissmann, F;Abel, L;Franco, JL;Bustamante, J;Casanova, JL;Boisson-Dupuis, S; Tuberculosis in otherwise healthy adults with inherited TNF deficiency Nature 2024-08-28 [PMID: 39198650] (Bioassay, Human) Bioassay Human
Kang, W;Xu, Q;Dong, H;Wang, W;Huang, G;Zhang, J; Eriodictyol attenuates osteoarthritis progression through inhibiting inflammation via the PI3K/AKT/NF-?B signaling pathway Scientific reports 2024-08-14 [PMID: 39143134] (Bioassay, Human) Bioassay Human
López-Bermudo, L;Moreno-Chamba, B;Salazar-Bermeo, J;Hayward, NJ;Morris, A;Duncan, GJ;Russell, WR;Cárdenas, A;Ortega, Á;Escudero-López, B;Berná, G;Martí Bruña, N;Duncan, SH;Neacsu, M;Martin, F; Persimmon Fiber-Rich Ingredients Promote Anti-Inflammatory Responses and the Growth of Beneficial Anti-Inflammatory Firmicutes Species from the Human Colon Nutrients 2024-08-01 [PMID: 39125398] (Bioassay, Human) Bioassay Human
Tomero-Sanz, H;Jiménez-Heffernan, JA;Fernández-Chacón, MC;Cristóbal-García, I;Sainz de la Cuesta, R;González-Cortijo, L;López-Cabrera, M;Sandoval, P; Detection of Carcinoma-Associated Fibroblasts Derived from Mesothelial Cells via Mesothelial-to-Mesenchymal Transition in Primary Ovarian Carcinomas Cancers 2024-07-29 [PMID: 39123425] (Bioassay, Human) Bioassay Human
Show All 601 Publications.

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Bioinformatics

Gene Symbol IL1B
Uniprot