![Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Western-Blot-NBP2-45312-img0005.jpg "Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).")
| Reactivity | Hu, Pm, Mu(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SPM288 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11419) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Activated human peripheral blood mononuclear cells |
| Localization | Cell Surface |
| Specificity | This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HLA-DRB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
|
| Publications |
|
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HLA DRB1 Antibody (NBP2-45312)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunohistochemistry-Paraffin-NBP2-45312-img0004.jpg "Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).")
![Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Flow-Cytometry-NBP2-45312-img0002.jpg "Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunohistochemistry-Paraffin-NBP2-45312-img0001.jpg "Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).")
![Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with APC (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HLA-DRB1-Antibody-SPM288-Immunofluorescence-NBP2-45312-img0003.jpg "Immunofluorescence: HLA DRB1 Antibody (SPM288) [NBP2-45312] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with APC (Green). The nuclear counterstain is Red Dot (Red)")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Neutralization TRAILR1/TNFRSF10A Antibody [Unconjugated]](https://images.novusbio.com/images2/TRAIL_R1_AF347_Block_Neutralize_875.jpg)
![Western Blot TRAILR1/TNFRSF10A Antibody [Unconjugated]](https://images.novusbio.com/images2/TRAIL_R1_AF347_Western_Blot_5303.jpg)
![Immunohistochemistry TRAILR1/TNFRSF10A Antibody [Unconjugated]](https://images.novusbio.com/images2/TRAIL_R1_AF347_Immunohistochemistry_17355.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
