We have purchased the antibody NB100-64775 HLA ABC Antibody (W6/32) 0.25mg. To date we have completed over 40 reactions and haven't found a single positive output. Can you please send me the flow cytometry images generated by this antibody??

Name: HLA ABC Antibody (W6/32)
Brand: Novus Biologicals
SKU: NB100-64775
Rating: 4.5 (2 reviews)

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Accepted Answer

I'm sorry to hear about the dificulty you have been having. Please refer to the images section of the datasheet. There are also many publications on the product page for your review. I did find PubMeb 21123379 links to a free PMC article that talks about this clone being used in Flow Cytometry. We also offer many conjugated forms of this antibody that you may be interested in. You can always contact technical@novusbio.com with any additional questions.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA, Flow, Func, IHC, IP, CyTOF-ready, IHC-P (-)
Clone	W6/32
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-64775AF350

Alexa Fluor 405 NB100-64775AF405

Alexa Fluor 488 NB100-64775AF488

Alexa Fluor 532 NB100-64775AF532

Alexa Fluor 594 NB100-64775AF594

Alexa Fluor 647 NB100-64775AF647

Alexa Fluor 700 NB100-64775AF700

Alexa Fluor 750 NB100-64775AF750

Allophycocyanin NB100-64775APC

Biotin NB100-64775B

DyLight 350 NB100-64775UV

DyLight 405 NB100-64775V

DyLight 488 NB100-64775G

DyLight 550 NB100-64775R

DyLight 594 NB100-64775DL594

DyLight 650 NB100-64775C

DyLight 680 NB100-64775FR

DyLight 755 NB100-64775IR

FITC NB100-64775F

HRP NB100-64775H

Janelia Fluor 525 NB100-64775JF525

Janelia Fluor 549 NB100-64775JF549

Janelia Fluor 646 NB100-64775JF646

Janelia Fluor 669 NB100-64775JF669

PE NB100-64775PE

PerCP NB100-64775PCP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64775AF350
Alexa Fluor 405	NB100-64775AF405
Alexa Fluor 488	NB100-64775AF488
Alexa Fluor 532	NB100-64775AF532
Alexa Fluor 594	NB100-64775AF594
Alexa Fluor 647	NB100-64775AF647
Alexa Fluor 700	NB100-64775AF700
Alexa Fluor 750	NB100-64775AF750
Allophycocyanin	NB100-64775APC
Biotin	NB100-64775B
DyLight 350	NB100-64775UV
DyLight 405	NB100-64775V
DyLight 488	NB100-64775G
DyLight 550	NB100-64775R
DyLight 594	NB100-64775DL594
DyLight 650	NB100-64775C
DyLight 680	NB100-64775FR
DyLight 755	NB100-64775IR
FITC	NB100-64775F
HRP	NB100-64775H
Janelia Fluor 525	NB100-64775JF525
Janelia Fluor 549	NB100-64775JF549
Janelia Fluor 646	NB100-64775JF646
Janelia Fluor 669	NB100-64775JF669
PE	NB100-64775PE
PerCP	NB100-64775PCP

Immunogen	Purified human tonsil lymphocyte membranes
Specificity	NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	HLA-A
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

HLA ABC Antibody (W6/32)

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Publications for HLA ABC Antibody (NB100-64775)(31)

Reviews for HLA ABC Antibody (NB100-64775) (2) 4.52

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Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	No Preservative
Concentration	1 mg/ml
Purity	Protein A purified

Publications using NB100-64775	Applications	Species
Yanagisawa M, Nagamatsu T, Kurano M et al. Upregulation of autotaxin by oxidative stress via Nrf2 activation: a novel insight into the compensation mechanism in preeclamptic placenta Journal of Reproductive Immunology 2023-09-01 [PMID: 37837670]
Phulphagar KM, Ctortecka C, Jacome ASV et al. Sensitive, High-Throughput HLA-I and HLA-II Immunopeptidomics Using Parallel Accumulation-Serial Fragmentation Mass Spectrometry Molecular & Cellular Proteomics 2023-06-01 [PMID: 37142057]
Phulphagar KM, Ctortecka C, Vaca Jacome AS et al. Sensitive, high-throughput HLA-I and HLA-II immunopeptidomics using parallel accumulation-serial fragmentation mass spectrometry bioRxiv : the preprint server for biology 2023-03-18 [PMID: 36993564] (IP, Human)	IP	Human
Mimura N, Nagamatsu T, Morita K et al. Suppression of human trophoblast syncytialization by human cytomegalovirus infection Placenta 2021-12-11 [PMID: 34933151] (MCS, Human)	MCS	Human
JGM Strijker, R Pscheid, E Drent, JJF van der Ho, B Koopmans, K Ober, SR van Hooff, WM Kholosy, AM Cornel, C Coomans, A Bisso, MM van Loenen, JJ Molenaar, J Wienke alphabeta-T Cells Engineered to Express gammadelta-T Cell Receptors Can Kill Neuroblastoma Organoids Independent of MHC-I Expression Journal of personalized medicine, 2021-09-17;11(9):. 2021-09-17 [PMID: 34575700] (B/N)	B/N
Javeed N, Her TK, Brown MR Et al. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell reports 2021-08-24 [PMID: 34433033] (IHC-P, Mouse)	IHC-P	Mouse
Darwish M, Wichner S, Li J et al. High-throughput identification of conditional MHCI ligands and scaled-up production of conditional MHCI complexes Protein science : a publication of the Protein Society 2021-04-11 [PMID: 33840137]
Mourier B, Labadie P, Desmet M et al. Combined spatial and retrospective analysis of fluoroalkyl chemicals in fluvial sediments reveal changes in levels and patterns over the last 40 years. Environmental pollution (Barking, Essex : 1987) 2019-11-08 [PMID: 31434189]
Wang Y, Huang J et al. The Plasticity of Mesenchymal Stem Cells in Regulating Surface HLA-I. iScience 2019-05-31 [PMID: 31030183] (WB, Human)	WB	Human
Nevens D, Duprez F, Daisne JF et al. Reduction of the dose of radiotherapy to the elective neck in head and neck squamous cell carcinoma; a randomized clinical trial. Effect on late toxicity and tumor control. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2017-09-28 [PMID: 27528118]
Jones, DC et al. 2011 HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding. J Immunol 186: 2990-7. 2011-01-01 [PMID: 21270408]
Zuo, J et al. The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways. J Virol 85: 1604-14. 2011-01-01 [PMID: 21123379]
Fujita, Y et al. Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice. Proc Natl Acad Sci U S A 107: 14345-50. 2010-01-01 [PMID: 20660747]
Narita, M et al. WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase. Int J Med Sci 7: 72-81. 2010-01-01 [PMID: 20428337]
Vitadello, M et al. Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation. Arthritis Res Ther 12: R52. 2010-01-01 [PMID: 20334640]
Hinrichs, J et al. The nature of peptides presented by an HLA class I low expression allele. Haematologica 95: 1373-80. 2010-01-01 [PMID: 20220067]
Spentzou, A et al. Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates. J Infect Dis 201: 720-9. 2010-01-01 [PMID: 20132004]
Grotzke, JE et al. The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle. PLoS Pathog 5: e1000374. 2009-01-01 [PMID: 19360129]
Koene G, Arts-Hilkes et al. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss Hum Immunol 2004-01-01 [PMID: 15603873] Details: This citation used the FITC version of this antibody.
Dressel, R et al. Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes. Cancer Res 63: 8212-8220. 2003-01-01 [PMID: 14678977]
Ishitani A, Sageshima N, Lee N et al. Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol. 2003-08-01 [PMID: 12874228]
Goldman-Wohl D, Ariel I et al. A study of human leukocyte antigen G expression in hydatidiform moles Am J Obstet Gynecol 2001-01-01 [PMID: 11518912] (IHC-Fr) Details: This citation used the FITC version of this antibody.	IHC-Fr
Yoshino N et al. Exp. Anim. (Tokyo) 49:97. 2000-01-01 [PMID: 10889948]
Jacobsen, C N et al. Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet Immunol Immunopathol 39: 461-466. 1993-01-01 [PMID: 8116221]
Verbeek, M M et al. T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions. J Immunol 154: 5876-5884. 1995-01-01 [PMID: 7538536]
Brodsky, F M Parham, P. Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies. Immunogenetics 15: 151-166. 1982-01-01 [PMID: 6174442]
Stern PL, Beresford N et al. Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast. J Immunol. 138(4):1088-91. 1987-01-01 [PMID: 3543121]
Tanabe, M et al. Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol 148: 3203-3209. 1992-01-01 [PMID: 1374450]
Barnstable, C J et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell 14: 9-20. 1978-01-01 [PMID: 667938]
Parasar P, Wilhelm A, Davis WC et al. A non-recoverable hybridoma limits the production of monoclonal antibodies against bovine trophoblast non-classical NC3*00101 protein Indian Journal of Experimental Biology (ELISA, Mouse)	ELISA	Mouse
Neefjes, J J et al. A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347. 1986-01-01 [PMID: 3458670]
Show All 31 Publications. Collapse Publications.

	Verified Customer 01/31/2022
Application:	IHC-Fr
Species:	Mouse

	Verified Customer 02/28/2014
Application:	Flow
Species:	Human