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Nephrin Antibody [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 594

Order Details

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Nephrin Antibody [Alexa Fluor® 594] Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. Amino Acid Sequence: EPDSLPFELRGHLV
Specificity
Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). NBP1-77303 can detect isoforms of human, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPHS1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nephrin Antibody [Alexa Fluor® 594]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

The Nephrin gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NPHS1