Tight Junction Protein 1 Antibody

Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: B/N, ICC/IF, IF/IHC, IHC, IHC-P, Simple Western, WB.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using NBP1-85047	Applications	Species
Rion J Wendland, Budd A Tucker, Kristan S Worthington Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells Stem Cells Translational Medicine 2024-06-01 [PMID: 38560893]
Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse)	IHC-P	Mouse
Ji S, You Y, Peng B et al. Multi-omics analysis reveals the metabolic regulators of duodenal low-grade inflammation in a functional dyspepsia model Frontiers in Immunology 2022-08-24 [PMID: 36091013] (WB)	WB
Rosu GC, Mateescu VO, Simionescu A et al. Subtle vascular and astrocytic changes in the brain of coronavirus disease 2019 (COVID-19) patients European Journal of Neurology 2022-12-01 [PMID: 36056566] (B/N)	B/N
Yang AM, Lin CY, Liu SH et al. Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis Frontiers in Microbiology 2022-06-23 [PMID: 35814685] (IHC)	IHC
Fu Q, Lin Q, Chen D et al. beta-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge BMC veterinary research 2022-04-19 [PMID: 35440001] (WB, ICC/IF)	WB, ICC/IF
Song K, Zeng X, Xie X et al. Dl-3-n-butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance Stroke and vascular neurology 2022-01-31 [PMID: 35101948] (WB, Rat)	WB	Rat
Spindler LM, Feuerhake A, Ladel S et al. Nano-in-Micro-Particles Consisting of PLGA Nanoparticles Embedded in Chitosan Microparticles via Spray-Drying Enhances Their Uptake in the Olfactory Mucosa Frontiers in Pharmacology 2021-09-01 [PMID: 34539414] (IHC)	IHC
Lassiter R, Merchen Td, Fang X, Wang Y Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation Frontiers in immunology 2021-07-07 [PMID: 34305900] (IHC-P)	IHC-P
Manon Boul, Nassima Benzoubir, Antonietta Messina, Rasta Ghasemi, Ismail Ben Mosbah, Jean-Charles Duclos-Vallée, Anne Dubart-Kupperschmitt, Bruno Le Pioufle A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation Scientific Reports 2021-07-07 [PMID: 34234159]
Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells Nat Methods 2021-05-04 [PMID: 33941937] (Simple Western)	Simple Western
Abbassi L, El-Hayek S, Carvalho KF et al. Epidermal growth factor receptor signaling uncouples germ cells from the somatic follicular compartment at ovulation Nature communications 2021-03-04 [PMID: 33664246] (ICC/IF, Mouse)	ICC/IF	Mouse
Hahn L, Helmrich N, Herebian D et al. IL-13 as Target to Reduce Cholestasis and Dysbiosis in Abcb4 Knockout Mice Cells 2020-08-24 [PMID: 32846954]
Su C, Liu S, Ma X et al. Decitabine attenuates dextran sodium sulfate?induced ulcerative colitis through regulation of immune regulatory cells and intestinal barrier International Journal of Molecular Medicine 2020-05-18 [PMID: 32468024] (ICC/IF)	ICC/IF
Liu X, Zhang B, Liu H et al. Determination of the available energy values and amino acid digestibility of Flammulina velutipes stem waste and its effects on carcass trait and meat quality fed to growing-finishing pigs J Anim Sci Biotechnol 2020-05-04 [PMID: 32391146] (WB)	WB
Caron Jerome, Pene Veronique, Tolosa Laia et al. Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection. Stem Cell Research & Therapy 2019-07-29 [PMID: 31358055] (ICC/IF)	ICC/IF
Coffin KM, Liu J, Warren TK et al. Persistent Marburg Virus Infection in the Testes of Nonhuman Primate Survivors. Cell Host Microbe 2018-09-12 [PMID: 30173956] (ICC/IF, Human)	ICC/IF	Human
Liu M, Yang J, Zhang Y et al. ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism. Clin. Cancer Res. 2018-04-03 [PMID: 29615456] (Human)		Human
McIntyre BA, Alev C, Mechael R et al. Expansive Generation of Functional Airway Epithelium From Human Embryonic Stem Cells. Stem Cells Transl Med 2014-01-01 [PMID: 24300555] (IF/IHC, Human)	IF/IHC	Human
Show All 20 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: James Xiao	ICC	Rat	12/10/2020	View Summary Application Immunocytochemistry Sample Tested Epithelial cells Species Rat Lot 000001836
Enlarge	5 reviewed by: Fabio Marongiu	ICC	Human	08/29/2017	View Summary Application Immunocytochemistry Sample Tested ARPE-19 cells Species Human Lot C105864 Comments Comments Fixative 4% PFA Blocking Goat Serum Dilution 1:200 Incubation 1h @ RT
Enlarge	4 reviewed by: James Xiao	WB	Mouse	06/22/2017	View Summary Application Western Blot Sample Tested Primary Mouse Lung cells Species Mouse
Enlarge	5 reviewed by: James Xiao	ICC/IF	Mouse	10/13/2016	View Summary Application Other Sample Tested Primary mouse lung endotelial cells Species Mouse Lot B10562
Enlarge	4 reviewed by: Verified Customer	ICC	Other	02/02/2016	View Summary Application Immunocytochemistry Sample Tested Pig trabecular meshwork cells Species Other

Array NBP1-85047

• Tight Junction Protein 1 Antibodies
• Tight Junction Protein 1 Lysate
• Tight Junction Protein 1 Proteins