Tight Junction Protein 2 Antibody

TJP2 - tight junction protein 2 (zona occludens 2). embers of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IF/IHC, WB.

Showing Publications 1 - 4 of 4.

Publications using NBP1-86850	Applications	Species
Chen YH, Chen CJ, Wang SJ et al. Downregulation of tight junction protein zonula occludens-2 and urothelium damage in a cyclophosphamide-induced mouse model of cystitis. Taiwan J Obstet Gynecol 2018-06-01 [PMID: 29880173] (IF/IHC, Mouse)	IF/IHC	Mouse
Hsiao HM, Fernandez R, Tanaka S et al. Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1B. J. Clin. Invest. 2018-07-02 [PMID: 29781811] (WB, Mouse)	WB	Mouse
Chen HY, Chen CJ, Chen WC et al. A promising protein responsible for overactive bladder in ovariectomized mice. Taiwan J Obstet Gynecol. 2017-04-01 [PMID: 28420508] (IF/IHC, Mouse)	IF/IHC	Mouse
Gonzalez-Andrades M, Garzon I, Gascon MI et al. Sequential development of intercellular junctions in bioengineered human corneas. J Tissue Eng Regen Med 2009-08-01 [PMID: 19415764]

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Akihiro Asai	IP	Human	06/18/2021	View Summary Application Immunoprecipitation Sample Tested 293 whole cell lysate Species Human Lot 000007801 Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.
Enlarge	3 reviewed by: Verified Customer	IHC-P	Human	04/11/2019	View Summary Application Immunohistochemistry-Paraffin Sample Tested human skin Species Human Lot A35234
Enlarge	5 reviewed by: James Xiao	ICC	Mouse	05/17/2017	View Summary Application Immunocytochemistry Sample Tested Frozen Tissue,Mouse lung frozen section Species Mouse Comments Comments Lung frozen section was blocked and stained with TJP-2 @ 1:100 dilution for a hour. A secondary Alexa 555-conjugated goat anti-rabbit antibody was used for labeling. Section was aso co-stained with CD31 antibody in conjunction with an Alexa 488-conjugated antibody. Nuclei was revealed by DAPU staining.
Enlarge	5 reviewed by: James Xiao	WB	Mouse	05/11/2017	View Summary Application Western Blot Sample Tested Primary mouse lung endothelial cells Species Mouse Comments Comments The antibody was diluted in PBST contained 5% milk at 1:1000. The secondary antibody was diluted at 1:5000.
Enlarge	5 reviewed by: James Xiao	Immunocytochemistry/Immunofluorescence	Mouse and Primary mouse lung endothelial cells	10/13/2016	View Summary Application Immunocytochemistry/Immunofluorescence Sample Tested Primary mouse lung endothelial cells Species Mouse and Primary mouse lung endothelial cells Lot A35234

Array NBP1-86850

• Tight Junction Protein 2 Antibodies
• Tight Junction Protein 2 ELISA Kits
• Tight Junction Protein 2 Lysate
• Tight Junction Protein 2 Proteins