PSD-95 Antibody (6G6-1C9) - BSA Free

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Invertebrate, Mollusc.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, WB.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using NB300-556	Applications	Species
Kovacs A, Vermeer DW, Madeo M, Reavis H Tumor-infiltrating nerves create an electro-physiologically active microenvironment and contribute to treatment resistance bioRxiv 2020-01-01 (ICC/IF, Mouse)	ICC/IF	Mouse
Muangsanit P, Chailangkarn T, Tanwattana N et al. Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection Frontiers in Cellular and Infection Microbiology 2023-08-25 [PMID: 37692167] (ICC/IF)	ICC/IF
Scaduto P, Lauterborn JC, Cox CD et al. Functional excitatory to inhibitory synaptic imbalance and loss of cognitive performance in people with Alzheimer's disease neuropathologic change Acta neuropathologica 2022-12-20 [PMID: 36538112] (FLOW, Human) Details: Dilution used in flow 1:80	FLOW	Human
de Lima Marcolin M Influence of adolescent social instability stress on the intake of ethanol and sucrose in a rodent model Thesis (WB, Rat)	WB	Rat
Jun Noguchi, Satoshi Watanabe, Tomofumi Oga, Risa Isoda, Keiko Nakagaki, Kazuhisa Sakai, Kayo Sumida, Kohei Hoshino, Koichi Saito, Izuru Miyawaki, Eriko Sugano, Hiroshi Tomita, Hiroaki Mizukami, Akiya Watakabe, Tetsuo Yamamori, Noritaka Ichinohe Altered projection-specific synaptic remodeling and its modification by oxytocin in an idiopathic autism marmoset model Communications Biology 2024-05-27 [PMID: 38802535]
Kumar S, Orlov E, Gowda P et al. Synaptosome microRNAs regulate synapse functions in Alzheimer's disease NPJ genomic medicine 2022-08-08 [PMID: 35941185] (WB, Mouse)	WB	Mouse
Zarate N, Intihar T, Yu D Et al. Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density preprints 2021-11-04 [PMID: 34884918] (WB, Mouse)	WB	Mouse
Ma KG, Hu HB, Zhou JS Et al. Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice Experimental neurology 2021-11-05 [PMID: 34748756] (WB, Mouse)	WB	Mouse
Bieler M, Hussain S, Daaland ESB et al. Changes in concentrations of NMDA receptor subunit GluN2B, Arc and syntaxin-1 in dorsal hippocampus Schaffer collateral synapses in a rat learned helplessness model of depression The Journal of comparative neurology 2021-04-12 [PMID: 33843051]
Diaz A, Merino P, McCann P et al. Urokinase-type plasminogen activator promotes N-cadherin-mediated synaptic recovery in the ischemic brain Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2021-03-24 [PMID: 33757316]
Diaz A, Torre E, Yepes M. Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex BIO-PROTOCOL 2021-01-20 [PMID: 33732785] (B/N)	B/N
O'grady BJ, Balotin KM, Bosworth AM et al. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks BioRxiv ACS Biomater Sci Eng 2020-12-15 [PMID: 33320550]
Sulistomo HW, Nemoto T, Kage Y et al. Fhod3 Controls the Dendritic Spine Morphology of Specific Subpopulations of Pyramidal Neurons in the Mouse Cerebral Cortex Cerebral cortex (New York, N.Y. : 1991) 2020-11-30 [PMID: 33251537]
Zhang N, Zhang Z, He R et al. GLAST-CreER(T2) mediated deletion of GDNF increases brain damage and exacerbates long-term stroke outcomes after focal ischemic stroke in mouse model Glia 2020-06-04 [PMID: 32497340]
Ritzel RM, Li Y, He J Sustained neuronal and microglial alterations are associated with diverse neurobehavioral dysfunction long after experimental brain injury Neurobiol Dis. [PMID: 31843705] (FLOW, Mouse)	FLOW	Mouse
Marcolin ML, Baumbach JL, Hodges TE, McCormick CM The effects of social instability stress and subsequent ethanol consumption in adolescence on brain and behavioural development in male rats Alcohol 2019-08-26 [PMID: 31465790] (WB, Rat)	WB	Rat
Hodges TE, Louth EL, Bailey CDC, McCormick CM. Adolescent social instability stress alters markers of synaptic plasticity and dendritic structure in the medial amygdala and lateral septum in male rats. Brain Struct Funct. 2018-11-23 [PMID: 30467598] (Rat)		Rat
Parker SS, Moutal A, Cai S et al. High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. eNeuro 2018-09-27 [PMID: 30263951] (ICC/IF, Mouse)	ICC/IF	Mouse
Simone JJ, Baumbach JL, McCormick CM. Sex-specific effects of CB1 receptor antagonism and stress in adolescence on anxiety, corticosterone concentrations, and contextual fear in adulthood in rats Int. J. Dev. Neurosci. 2018-10-01 [PMID: 30063953] (WB, Rat)	WB	Rat
Simone JJ, Baumbach JL, McCormick CM. Effects of CB1 receptor antagonism and stress exposures in adolescence on socioemotional behaviours, neuroendocrine stress responses, and expression of relevant proteins in the hippocampus and prefrontal cortex in rats Neuropharmacology. 2017-10-29 [PMID: 29092785] (WB, Rat)	WB	Rat
Hussain S, Ringsevjen H, Egbenya DL et al. SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus. Front Mol Neurosci 2016-02-05 [PMID: 26903802]
Hussain S, Davanger S. Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits. PLoS One 2015-01-01 [PMID: 26488171] (WB)	WB
Naskar S, Wan H, Kemenes G. pT305-CaMkII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning. Nat Commun. 2014-05-30 [PMID: 24875483] (IHC-Fr, Mollusc)	IHC-Fr	Mollusc
Giribaldi F, Milanese M, Bonifacino T, Anna Rossi PI, Di Prisco S, Pittaluga A, Tacchetti C, Puliti A, Usai C, Bonanno G. Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis. Neuropharmacology. 2012-05-23 [PMID: 22634363] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Reissner KJ, Boyle HD, Ye X, Carew TJ. Aplysia synapse associated protein (APSAP): identification, characterization, and selective interactions with Shaker-type potassium channels. J Neurochem 2008-05-01 [PMID: 18182049] (WB, ICC/IF, Invertebrate)	WB, ICC/IF	Invertebrate
Fogel AI, Akins MR, Krupp AJ et al. SynCAMs organize synapses through heterophilic adhesion. J Neurosci. 2007-11-01 [PMID: 18003830]
Furuyashiki T et al. Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus. J Neurosci 19:109-18. 1999-01-01 [PMID: 9870943]
Zhang W et al. Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. J Neurosci 19:96-108. 1999-01-01 [PMID: 9870942]
Gomperts SN. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62. 1996-01-01 [PMID: 8625403]
Patton BL et al. Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- dephosphokinase with complementary phosphorylation site-specific antibodies. Mol Biol Cell 4:159-72. 1993-01-01 [PMID: 8443414]
Kornau HC et al. Domain interaction between NMDA receptor subunits the postsynaptic density protein PSD-95. Science 269:1737-40. 1995-01-01 [PMID: 7569905]
Show All 31 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Janosch Heller	ICC	Rat	06/27/2017	View Summary Application Immunocytochemistry Sample Tested rat cortical culture (3 weeks old) Species Rat Comments Comments Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT Blocking Solution & Duration: 10% donkey serum in PBS with 0.1% triton, 30 minutes RT Primary Antibody Diluent and Dilutions Tested: 1:650 in 5% donkey serum in PBS, over night 4°C Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse Alexa647, 1:1250 in PBS, 2 hours, RT

Array NB300-556

• PSD-95 Antibodies
• PSD-95 Lysate
• PSD-95 Proteins