NMDAR1 Antibody (R1JHL) - Azide and BSA Free

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: B/N, FLOW, ICC/IF, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NB300-118	Applications	Species
Hossen E, Funahashi Y, Faruk M et al. Rho-Kinase/ROCK Phosphorylates PSD-93 Downstream of NMDARs to Orchestrate Synaptic Plasticity International Journal of Molecular Sciences 2022-12-26 [PMID: 36613848] (WB, Mouse)	WB	Mouse
Yamahashi Y, Lin YH, Mouri A et al. Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-beta-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning Molecular psychiatry 2022-06-03 [PMID: 35665767] (WB)	WB
Rahim M, Yoon Y, Dimovasili C et al. PS1 familial Alzheimer disease mutants inactivate EFNB1- and BDNF-dependent neuroprotection against excitotoxicity by affecting neuroprotective complexes of NMDA receptor Brain Commun 2020-10-02 [PMID: 33005890]
Wang, Y, Kerrisk Campbell, M Et al. PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function. Sci Rep 2020-07-02 [PMID: 32616769] (FLOW, Rat) Details: Citation using the Alexa Fluor 647 format of this antibody.	FLOW	Rat
Staats KA, Seah C, Sahimi A, Wang Y Small molecule inhibition of PIKFYVE kinase rescues gain-and loss-of-function C9ORF72 ALS/FTD disease processes in vivo bioRxiv 2019-06-28 (WB, Mouse)	WB	Mouse
Perri RGB, Mantello AG, Rosa DS, Beleboni RO. Silencing of the GluN1-NMDA Glutamate Receptor Subunit by Intranasal siRNA Increases the Latency Time for Seizures in the Pilocarpine Rodent Model of Epilepsy Pharmaceuticals (Basel) 2022-11-26 [PMID: 36558924] (B/N)	B/N
Shi Y, Hung ST, Rocha G et al. Identification and therapeutic rescue of autophagosome and glutamate receptor defects in C9ORF72 and sporadic ALS neurons JCI Insight 2019-07-16 [PMID: 31310593] (WB, Human)	WB	Human
Stewart M, Lau P, Banks G et al. Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities Dis Model Mech 2019-02-22 [PMID: 30692144] (WB, Mouse)	WB	Mouse
Stewart ME. Development of high-throughput, non-invasive behavioural and cognitive tests in mice to uncover new mechanisms of abnormal cognition and behaviour Thesis 1905-07-10 (Mouse)		Mouse
Mireia Carcolé, Sami Kummer, Leonor Gonçalves, Daniel Zamanillo, Manuel Merlos, Anthony H. Dickenson, Begoña Fernández‐Pastor, David Cabañero, Rafael Maldonado Sigma‐1 receptor modulates neuroinflammation associated with mechanical hypersensitivity and opioid tolerance in a mouse model of osteoarthritis pain British Journal of Pharmacology 2019-09-12 [PMID: 31332781]
Shi Y, Lin S, Staats KA, Li Y. Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Nat. Med. 2018-02-05 [PMID: 29400714] (Human)		Human
Gorska-Ponikowska M, Perricone U, Kuban-Jankowska A et al. 2-Methoxyestradiol Impacts on Amino Acids-mediated Metabolic Reprogramming in Osteosarcoma Cells by Interaction with NMDA Receptor. J. Cell. Physiol. 2017-03-06 [PMID: 28262924] (WB, Human)	WB	Human
Hewett SJ, Shi J, Gong Y et al. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: Opposing roles for the transcription factors CREB and Sp1. J. Biol. Chem. 2016-11-14 [PMID: 27875294] (WB, Mouse)	WB	Mouse
Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015-12-04 [PMID: 26635527] (IHC-P, Mouse)	IHC-P	Mouse
Plaza-Zabala A, Li X, Milovanovic M et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions. Neurosci Lett. 2013-12-17 [PMID: 24262606] (WB, Rat)	WB	Rat
Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011-08-23 [PMID: 21861871] (WB, Rat)	WB	Rat
Ferrario CR, Loweth JA, Milovanovic M et al. Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens Neurosci Lett 2011-03-01 [PMID: 21182898] (WB, Rat)	WB	Rat
Mashkina AP, Cizkova D, Vanicky I et al. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol 2010-08-01 [PMID: 20414717] (IHC-Fr, ICC/IF, Rat, Human)	IHC-Fr, ICC/IF	Rat, Human
Garraway SM, Xu Q, Inturrisi CE. siRNA-mediated knockdown of the NR1 subunit gene of the NMDA receptor attenuates formalin-induced pain behaviors in adult rats. J Pain 2009-04-01 [PMID: 19185544] (Rat)		Rat
Lim, G et al. Expression of spinal NMDA receptor PKC after chronic morphine is regulated by spinal glucorticoid receptor, J. Neurosci2005. [PMID: 16319314] (WB, ICC/IF, Rat)	WB, ICC/IF	Rat
Nagy, J et al. Differential alterations in the expression of NMDA recaptor subunits following chromic ethanol treatment in primary cultures of rat cortical hippocampal neurons. Neurochemistry International, 42(2003):35-43. 2002-01-01 [PMID: 12441166]
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Mouse	01/31/2018	View Summary Application Western Blot Sample Tested Mouse brain Species Mouse Lot BW1017c
Enlarge	4 reviewed by: Verified Customer	ICC	Mouse	08/04/2017	View Summary Application Immunocytochemistry Sample Tested Cultured hippocampal neurons Species Mouse Lot BW1016c
Enlarge	4 reviewed by: Verified Customer	IP	Mouse	08/04/2017	View Summary Application Immunoprecipitation Sample Tested Mice Brain Lysate Species Mouse Lot BW1016c
Enlarge	4 reviewed by: Verified Customer	WB	Human	08/04/2017	View Summary Application Western Blot Sample Tested 293T Species Human Lot WB1016c
Enlarge	4 reviewed by: Verified Customer	IP	Mouse	07/14/2017	View Summary Application Immunoprecipitation Sample Tested brain and spinal cord Species Mouse Lot BW1016c
Enlarge	4 reviewed by: Verified Customer	ICC	Mouse	07/14/2017	View Summary Application Immunocytochemistry Sample Tested Cortical neurons Species Mouse Lot BW1016c
Enlarge	4 reviewed by: Verified Customer	WB	Human	07/14/2017	View Summary Application Western Blot Sample Tested brain and spinal cord Species Human Lot BW1016c
	3 reviewed by: Janosch Heller	ICC	Rat	06/27/2017	View Summary Application Immunocytochemistry Sample Tested rat cortical culture (3 weeks old) Species Rat Comments Comments Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT
Enlarge	4 reviewed by: Verified Customer	IF	Mouse	11/26/2015	View Summary Application Immunofluorescence Sample Tested Mouse neurons Species Mouse
Enlarge	4 reviewed by: Verified Customer	ICC	Mouse	11/26/2015	View Summary Application Immunocytochemistry Sample Tested Mouse neurons Species Mouse Lot BW714r
Enlarge	5 reviewed by: Verified Customer	IP	Mouse	10/16/2015	View Summary Application Immunoprecipitation Sample Tested Mouse Brain Tissue Species Mouse Lot BW714r
Enlarge	5 reviewed by: Verified Customer	IP	Mouse	10/15/2015	View Summary Application Immunoprecipitation Sample Tested Mouse neuronal cultures Species Mouse Lot BW714r
Enlarge	5 reviewed by: Verified Customer	IP	Mouse	10/15/2015	View Summary Application Immunoprecipitation Sample Tested Mouse neurons Species Mouse Lot BW714r
	5 reviewed by: Fugui Li	IF	Human	09/30/2014	View Summary Application Immunofluorescence Sample Tested NR1 plasmid transfected HEK293T cells Species Human

Array NB300-118

• NMDAR1 Antibodies
• NMDAR1 ELISA Kit
• NMDAR1 Lysates
• NMDAR1 Proteins