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NMDAR2C Antibody - Azide and BSA Free

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Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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NMDAR2C Antibody - Azide and BSA Free Summary

Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Fusion protein from the N-terminal region of the NMDAR2C subunit. Accession # Q00961
Localization
Cerebellum
Specificity
Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor. Also labels the ~180 kDa NR2A and the ~180 kDa NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2C
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunoprecipitation 3 ul per 200 ug lysate
  • Western Blot 1:1000
Application Notes
This antibody cross reacts with both NR2A and NR2B, as such, results in immunohistochemistry are varied.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NMDAR2C Overexpression Lysate
Publications
Read Publications using
NB300-107 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute with 50 uL PBS to desired concentration.

Alternate Names for NMDAR2C Antibody - Azide and BSA Free

  • glutamate [NMDA] receptor subunit epsilon-3
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2C
  • GRIN2C
  • NMDA R, NR2C Subunit
  • NMDAR2C
  • NMDAR2CNR2C
  • N-methyl D-aspartate receptor subtype 2C
  • N-methyl-D-aspartate receptor subunit 2C
  • NR2C

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMDAR2C Antibody (NB300-107)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NMDAR2C Antibody (NB300-107). (Showing 1 - 1 of 1 FAQs).

  1. We have a note from 2005 on our internal datasheet from Novus stating that this antibody has only been tested for IHC on frozen tissue but I see that the Novus datasheet now lists IHC-P. Can you please confirm whether the antibody has been tested on paraffin-embedded tissue? If yes, is there a specific protocol, in particular for antigen retrieval?
    • NB300-107 has been validated in mouse for IHC-frozen, but not paraffin embedded sections yet.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GRIN2C
Entrez
Uniprot